Abstract
Even though organisms with squalene hopene cyclase activity involved in hopanoid synthesis has been reported earlier, their existence along with carotenoid synthesis is rarely reported. Here, we report the existence of hopanoid and C30 carotenoid biosynthetic pathway in Pseudomonas mendocina, the squalene hopene cyclase producing endophyte of the medicinal plant Murraya koenigii. The enzyme squalene hopene cyclase from Pseudomonas mendocina is involved in the synthesis of dehydrosqualene-mediated alternate pathway for carotenoid biosynthesis. The hopanoids are involved in membrane stability and integrity, and the carotene chromophores are involved in the photo protection of the cell. The orange-colored C30 carotenoid pigment 4–4′ diaponeurosporenic acid in the extracellular extract of Pseudomonas mendocina with squalene cyclase activity was detected by the combination of UV/Vis spectrometry, FTIR, and Mass Spectrometry. 4–4′ diaponeurosporenic acid could be traced as the end product of the carotenoid pathway and belonged to the xanthophyll group of carotenoids.
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Acknowledgements
The current study was supported by KSCSTE-SARD Programme, Kerala State Council for Science, Technology and Environment, DBT-MSUB Common Instrumentation Facility, School of Biosciences, and LC-MS/MS facility at Inter University Instrumentation Facility, School of Environmental Sciences, Mahatma Gandhi University Kottayam.
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Nair, I.M., Jayachandran, K. 4–4′ Diaponeurosporenic Acid, the C30 Carotenoid Pigment in Endophytic Pseudomonas Mendocina with Squalene Cyclase Activity. Curr Microbiol 77, 3473–3479 (2020). https://doi.org/10.1007/s00284-020-02180-3
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DOI: https://doi.org/10.1007/s00284-020-02180-3