1887

Journal of Medical Microbiology logo

Volume 69, Issue 10

Antifungal activity of etomidate against growing biofilms of fluconazole-resistant spp. strains, binding to mannoproteins and molecular docking with the ALS3 protein Free

Abstract

This study evaluated the effect of etomidate against biofilms of spp. and analysed through molecular docking the interaction of this drug with ALS3, an important protein for fungal adhesion. Three fluconazole-resistant fungi were used: , and . Growing biofilms were exposed to etomidate at 31.25–500 µg ml. Then, an ALS3 adhesive protein from was analysed through a molecular mapping technique, composed of a sequence of algorithms to perform molecular mapping simulation based on classic force field theory. Etomidate showed antifungal activity against growing biofilms of resistant and at all concentrations used in the study. The etomidate coupling analysis revealed three interactions with the residues of interest compared to hepta-threonine, which remained at the ALS3 site. In addition, etomidate decreased the expression of mannoproteins on the surface of . These results revealed that etomidate inhibited the growth of biofilms.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ALS3 , antifungal activity , biofilm , Candida spp. , etomidate and molecular docking
© 2020 The Authors
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001241
2020-09-07
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jmm/69/10/1221.html?itemId=/content/journal/jmm/10.1099/jmm.0.001241&mimeType=html&fmt=ahah

References

  1. Matthaiou DK, Christodoulopoulou T, Dimopoulos G. How to treat fungal infections in ICU patients. BMC Infect Dis 2015; 15:205 [View Article][PubMed]
     [Google Scholar]
  2. Pappas PG, Kauffman CA, Andes DR, Clancy CJ, Marr KA et al. Clinical practice guideline for the management of candidiasis: 2016 update by the infectious diseases Society of America. Clin Infect Dis 2016; 62:409–417 [View Article][PubMed]
     [Google Scholar]
  3. Pfaller MA, Andes DR, Diekema DJ, Horn DL, Reboli AC et al. Epidemiology and outcomes of invasive candidiasis due to non-albicans species of Candida in 2,496 patients: data from the Prospective Antifungal Therapy (PATH) registry 2004-2008. PLoS One 2014; 9:e101510 [View Article][PubMed]
     [Google Scholar]
  4. Calderone RA, Fonzi WA. Virulence factors of Candida albicans . Trends Microbiol 2001; 9:327–335 [View Article]
     [Google Scholar]
  5. Kullberg BJ, Arendrup MC. Invasive candidiasis. N Engl J Med 2015; 373:1445–1456 [View Article][PubMed]
     [Google Scholar]
  6. Li WS, Chen YC, Kuo SF, Chen FJ, Lee CH. The impact of biofilm formation on the persistence of candidemia. Front Microbiol 2018; 9:1196
     [Google Scholar]
  7. Kojic EM, Darouiche RO. Candida infections of medical devices. Clin Microbiol Rev 2004; 17:255–267 [View Article][PubMed]
     [Google Scholar]
  8. Morschhäuser J. Regulation of multidrug resistance in pathogenic fungi. Fungal Genet Biol 2010; 47:94–106 [View Article][PubMed]
     [Google Scholar]
  9. Hoyer LL, Cota E. Candida albicans agglutinin-like sequence (ALS) family vignettes: a review of als protein structure and function. Front Microbiol 2016; 7:280 [View Article][PubMed]
     [Google Scholar]
  10. Liu Y, Filler SG. Candida albicans ALS3, a multifunctional adhesin and invasin. Eukaryot Cell 2011; 10:168–173 [View Article][PubMed]
     [Google Scholar]
  11. Kioshima ES, Shinobu-Mesquita CS, Abadio AKR, Felipe MSS, Svidzinski TIE et al. Selection of potential anti-adhesion drugs by in silico approaches targeted to ALS3 from Candida albicans . Biotechnol Lett 2019; 41:1391–1401 [View Article][PubMed]
     [Google Scholar]
  12. de Andrade Neto JB, da Silva CR, Barroso FD, do Amaral Valente Sá LG, de Sousa Campos R et al. Synergistic effects of ketamine and azole derivatives on Candida spp. resistance to fluconazole. Future Microbiol 2020; 15:177–188 [View Article][PubMed]
     [Google Scholar]
  13. Keleş GT, Kurutepe S, Tok D, Gazi H, Dinç G. Comparison of antimicrobial effects of dexmedetomidine and etomidate-lipuro with those of propofol and midazolam. Eur J Anaesthesiol 2006; 23:1037–1040 [View Article][PubMed]
     [Google Scholar]
  14. Valente Sá LG, da Silva CR, S Campos Rde, de A Neto JB, Sampaio LS et al. Synergistic anticandidal activity of etomidate and azoles against clinical fluconazole-resistant Candida isolates. Future Microbiol 2019; 14:1477–1488 [View Article][PubMed]
     [Google Scholar]
  15. Pierce CG, Uppuluri P, Tristan AR, Wormley FL, Mowat E et al. A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing. Nat Protoc 2008; 3:1494–1500 [View Article][PubMed]
     [Google Scholar]
  16. Costa Silva RA, da Silva CR, de Andrade Neto JB, da Silva AR, Campos RS et al. In vitro anti-Candida activity of selective serotonin reuptake inhibitors against fluconazole-resistant strains and their activity against biofilm-forming isolates. Microb Pathog 2017; 107:341–348 [View Article][PubMed]
     [Google Scholar]
  17. de Aguiar Cordeiro R, Serpa R, Flávia Uchoa Alexandre C, de Farias Marques FJ, Vladia Silva de Melo C et al. Trichosporon inkin biofilms produce extracellular proteases and exhibit resistance to antifungals. J Med Microbiol 2015; 64:1277–1286 [View Article][PubMed]
     [Google Scholar]
  18. Ferreira LG, Dos Santos RN, Oliva G, Andricopulo AD. Molecular docking and structure-based drug design strategies. Molecules 2015; 20:13384–13421 [View Article][PubMed]
     [Google Scholar]
  19. Halgren TA. Merck molecular force field. I. basis, form, scope, parameterization, and performance of MMFF94. J Comput Chem 1996; 17:490–519 [View Article]
     [Google Scholar]
  20. Hanwell MD, Curtis DE, Lonie DC, Vandermeersch T, Zurek E et al. Avogadro: an advanced semantic chemical editor, visualization, and analysis platform. J Cheminform 2012; 4:17 [View Article][PubMed]
     [Google Scholar]
  21. Trott O, Olson AJ. AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J Comput Chem 2010; 31:455–461 [View Article][PubMed]
     [Google Scholar]
  22. Lin J, Oh S-H, Jones R, Garnett JA, Salgado PS et al. The peptide-binding cavity is essential for Als3-mediated adhesion of Candida albicans to human cells. J Biol Chem 2014; 289:18401–18412 [View Article][PubMed]
     [Google Scholar]
  23. Curvelo J, Barreto S, Portela MB, Alviano DS, Holandino C et al. Efeito do inibidor secretor de proteinase leucocitária (SLPI) NOS processos biológicos de Candida albicans: uma alternativa terapêutica?. Arch Oral Biol 2014; 59:928–937
     [Google Scholar]
  24. Tangcharoensathien V, Sattayawutthipong W, Kanjanapimai S, Kanpravidth W, Brown R et al. Antimicrobial resistance: from global agenda to national strategic plan, Thailand. Bull World Health Organ 2017; 95:599–603
     [Google Scholar]
  25. Uppuluri P, Chaturvedi AK, Srinivasan A, Banerjee M, Ramasubramaniam AK et al. Dispersion as an important step in the Candida albicans biofilm developmental cycle. PLoS Pathog 2010; 6:e1000828 [View Article][PubMed]
     [Google Scholar]
  26. Modrzewska B, Kurnatowski P. Adherence of Candida sp. to host tissues and cells as one of its pathogenicity features. Ann Parasitol 2015; 61:3–9[PubMed]
     [Google Scholar]
  27. Moraes DC, Curvelo JAR, Anjos CA, Moura KCG, Pinto MCFR et al. β-lapachone and α-nor-lapachone modulate Candida albicans viability and virulence factors. J Mycol Med 2018; 28:314–319
     [Google Scholar]
  28. Silva DR, Sardi JCO, Freires IA, Silva ACB, Rosalen PL. In silico approaches for screening molecular targets in Candida albicans: a proteomic insight into drug discovery and development. Eur J Pharmacol 2019; 842:64–69 [View Article][PubMed]
     [Google Scholar]
  29. Rodrigues-Vendramini FAV, Faria DR, Arita GS, Capoci IRG, Sakita KM et al. Antifungal activity of two oxadiazole compounds for the paracoccidioidomycosis treatment. PLoS Negl Trop Dis 2019; 13:e0007441 [View Article][PubMed]
     [Google Scholar]
  30. Rozada AM, Rodrigues FA, Sampiron EG, Seixas FA, Basso EA et al. Novel 4-methoxynaphthalene-N-acylhydrazones as potential for paracoccidioidomycosis and tuberculosis co-infection . Future Microbiol 2019; 14:587–598 [View Article][PubMed]
     [Google Scholar]
  31. Nadaf NH, Parulekar RS, Patil RS, Gade TK, Momin AA et al. Biofilm inhibition mechanism from extract of Hymenocallis littoralis leaves. J Ethnopharmacol 2018; 222:121–132 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001241
Loading
Antifungal activity of etomidate against growing biofilms of fluconazole-resistant Candida spp. strains, binding to mannoproteins and molecular docking with the ALS3 protein
J. Med. Microbiol. 69, 1221 (2020); https://doi.org/10.1099/jmm.0.001241
/content/journal/jmm/10.1099/jmm.0.001241
/content/journal/jmm/10.1099/jmm.0.001241
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error