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Evaluation of the efficiency of chlorpyrifos-ethyl remediation by Methylobacterium radiotolerans and Microbacterium arthrosphaerae using response of some biochemical biomarkers

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Abstract

This study reveals out detoxifying and antioxidant enzyme response of Gammarus pulex exposed/polluted to chlorpyrifos-ethyl insecticide before and after biodegradation/bioremediation by Methylobacterium radiotolerans and Microbacterium arthrosphaerae. Cytochrome P450 1A1, glutathione S-transferase, catalase, and superoxide dismutase activities in G. pulex exposed to chlorpyrifos-ethyl before and after bioremediation/biodegradation by these two bacteria during 24 and 96 h tested by using commercial ELISA kits. The activity of catalase enzyme was decreased depending on chlorpyrifos-ethyl before and after bioremediation/biodegradation the enzyme activity was increased repeatedly. Superoxide dismutase activity level increased after chlorpyrifos-ethyl exposure in 96 h (p > 0.05). Following bioremediation, superoxide dismutase enzyme activity decreased again during 24 h (p > 0.05) and increased during 96 h (p < 0.05). Statistical differences were not found in cytochrome P450 1A1 enzyme activity before and after the process (p > 0.05). No significant differences were determined during the activity of glutathione S-transferase in 24 h (p > 0.05). The activities of glutathione S-transferase were increased after exposure of chlorpyrifos-ethyl during 96 h. After bioremediation; the activity of glutathione S-transferase increased even more (p < 0.05). The results determined that activities of G. pulex at superoxide dismutase, catalase, and glutathione S-transferase are common biomarkers for revealing out the efficiency of bioremediation of chlorpyrifos-ethyl with these two types of soil bacteria.

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References

  • Agrawa A, Sharma B (2010) Pesticide induced oxidative stress in mammalian systems. Int J Biol Med Res 1(3):90–104

    Google Scholar 

  • Aksu O, Yildirim NC, Yildirim N, Danabas D, Danabas S (2015) Biochemical response of crayfish Astacus leptodactylus exposed to textile wastewater treated by indigenous white rot fungus Coriolus versicolor. Environ Sci Pollut Res 22:2987–2993

    Article  CAS  Google Scholar 

  • Altuntas I, Delibas N, Doguc DK, Ozmen S, Gultekin F (2003) Role of reactive oxygen species in organophosphate insecticide phosalone toxicity in erythrocytes in vitro. Toxicol in Vitro 17(2):153–157

    Article  CAS  Google Scholar 

  • Anwar WA (1997) Biomarker and human exposure to pesticides. Environ Health Perspect 105:801–806

    CAS  Google Scholar 

  • APHA (1998) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association, Washington DC

    Google Scholar 

  • Arias-Estevez M, Lopez-Periago E, Martinez-Carballo E, Simal-Gandara J, Mejuto JC, Garcia-Rio L (2008) The mobility and degradation of pesticides in soils and the pollution of groundwater resources. Agric Ecosyst Environ 123:247–260

    Article  CAS  Google Scholar 

  • Bacchetta C, Rossi AS, Ale A, Campana M, Parma J, Cazenave J (2014) Combined toxicological effects of pesticides: a fish multibiomarker approach. Ecol Indic 6:532–538

    Article  CAS  Google Scholar 

  • Barrett M (2000) The role of cytochrome P450 enzymes in herbicide metabolism. In: Cobbs AH, Kirkwood RC (eds) Herbicides and Their Mechanisms of Action. Sheffield Academic, Sheffield, pp 25–37

    Google Scholar 

  • Birkhoj M, Nellemann C, Jarfelt K, Jacobsen H, Andersen HR, Dalggard M, Vinggaard AM (2004) The combined antiandrogenic effects of five commonly used pesticides. Toxicol Appl Pharmacol 15(201):10–20

    Article  CAS  Google Scholar 

  • Boryslawskyj M, Garrood AC, Pearson JT (1988) Elevation of GST as a stress response to organochlorine compounds in Sphaerium corneum. Mar Environ Res 24:101–104

    Article  CAS  Google Scholar 

  • Boschin GD, Agostina A, Arnoldi A, Marotta E, Zanardini E, Negri M, Valle A, Sorlini C (2003) Biodegradation of chlorsulfuron and metsulfuron- methyl by aspergillus niger in laboratory conditions. J Environ Sci Health B 38(6):737–746

    Article  CAS  Google Scholar 

  • Cemeli E, Baumgartner A, Anderson D (2009) Antioxidants and the Comet assay. Mutat Res Rev Mutat Res 681(1):51–67

    Article  CAS  Google Scholar 

  • Cremlyn R (1991) Agrochemicals: preparation and mode of action. Wiley, Chichester

    Google Scholar 

  • De Lange HJ, Noordoven W, Murk AJ, Lürling MFLLW, Peeters ETHM (2006) Behavioural responses of Gammarus pulex (Crustacea, Amphipoda) to low concentrations of pharmaceuticals. Aquat Toxicol 78:209–216

    Article  CAS  Google Scholar 

  • Demirci O, Güven K, Asma D, Öğüt S, Uğurlu P (2018) Effects of endosulfan, thiamethoxam, and indoxacarb in combination with atrazine on multi-biomarkers in Gammarus kischineffensis. Ecotoxicol Environ Saf 147(218):749–758

    Article  CAS  Google Scholar 

  • Desaint S, Hartmann A, Parekh NR, Fournıer JC (2000) Genetic diversity of carbofuran-degrading soil bacteria. FEMS Microbiol Ecol 34(2):173–180

    Article  CAS  Google Scholar 

  • Dua M, Sıngh A, Sethunathan N, Johrı AK (2002) Biotechnology and bioremediation: successes and limitations. Appl Microbiol Biotechnol 59(2-3):143–152

    Article  CAS  Google Scholar 

  • Eaton DL, Daroff RB, Autrup H, Bridges J, Buffler P, Costa LG, Coyle J, McKhann G, Mobley WC, Nadel L, Neubert D, Schulte-Hermann R, Spencer PS (2008) Review of the toxicology of chlorpyrifos with an emphasis on human exposure and neurodevelopment. Crit Rev Toxicol 38:1–125

    Article  CAS  Google Scholar 

  • Erguven GO (2018) Comparison of some soil fungi in bioremediation of herbicide acetochlor under agitated culture media. Bull Environ Contam Toxicol 100:570–575

    Article  CAS  Google Scholar 

  • Erguven GO, Demir G (2019) Evaluation of the detoxification potential of Micrococcus strains and plants for bioremediate organochlorine herbicides. Int J Innov Approaches Agric Res 3(3):353–364

    Google Scholar 

  • Erguven GO, Yildirim N (2016) Efficiency of some soil bacteria for chemical oxygen demand reduction of synthetic chlorsulfuron solutions under agitated culture conditions. Cell Mol Biol 62(6):92–96

    CAS  Google Scholar 

  • Erguven GO, Yildirim N (2019) The evaluation of imidacloprid remediation in soil media by two bacterial strains. Curr Microbiol 76(12):1461–1466

    Article  CAS  Google Scholar 

  • Fang H, Wang Y, Gao C, Yan H, Dong B, Yu Y (2010) Isolation and characterization of Pseudomonas sp. CBW capable of degrading carbendazim. Biodegradation 21:939–946

    Article  CAS  Google Scholar 

  • Field J, Stams AM, Kato M, Schraa G (1995) Enhanced biodegradation of aromatic pollutants in cocultures of anaerobic and aerobic bacterial consortia. Anton Leeuw 67(1):47–77

    Article  CAS  Google Scholar 

  • Furukawa K (2003) Super bugs for bioremediation. Trends Biotechnol 21(5):187–190

    Article  CAS  Google Scholar 

  • Gerhardt A, Kienle C, Allan IJ, Greenwood R, Guigues N, Fouillac AM, Mills GA, Gonzalez C (2007) Biomonitoring with Gammarus pulex at the Meuse (NL), Aller (GER) and Rhine (F) rivers with the online Multispecies Freshwater Biomonitor®. J Environ Monit 9:979–985

    Article  CAS  Google Scholar 

  • Gui-Shan Z, Xiao-Ming J, Tian-Fan C, Xiao-Hang M, Yu-Hua Z (2005) Isolation and characterization of a new carbendazim-degrading Ralstonia sp. World J Microbiol Biotechnol 21:265–269

    Article  CAS  Google Scholar 

  • Gultekin F, Delibas N, Yasar S, Kilinc I (2001) In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and Vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats. Arch Toxicol 75:88–96

    Article  CAS  Google Scholar 

  • Hai DQ, Varga SI, Matkovics B (1997) Organophosphate effects of antioxidant system of carp (Cyprinus carpio) and catfish (Ictalurus nebulosus). Comp Biochem Physiol 117:83–88

    CAS  Google Scholar 

  • Hemingway J (2000) The molecular basis of two contrasting metabolic mechanisms of insecticide resistance. Insect Biochem Mol Biol 30:1009–1015

    Article  CAS  Google Scholar 

  • James MO (1989) Biotransformation and disposition of PAH in aquatic invertebrates. In: Varanasi V (ed) Metabolism of polycyclic aromatic hydrocarbons in the aquatic environment. CRC Press, Boca Raton

    Google Scholar 

  • Kono Y, Fridovich I (1982) Superoxide radicals inhibit catalase. J Biol Chem 257:5751–5754

    Article  CAS  Google Scholar 

  • Laemmli CM, Leveau JHJ, Zehnder AJB, Van der Meer JR (2000) Characterization of a second tfd gene cluster for chlorophenol and chlorocatechol metabolism on plasmid pJP4 in Ralstonia eutropha JMP134 (pJP4). J Bacteriol 182(15):4165–4172

    Article  CAS  Google Scholar 

  • Li X, Schuler MA, Berenbaum MR (2007) Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics. Annu Rev Entomol 52:231–253

    Article  CAS  Google Scholar 

  • Lima PL, Benassi JC, Pedrosa RC, Dal Magro J, Oliveira TB, Wilhelm FD (2006) Time course variations of DNA damage and biomarkers of oxidative stress in Tilapia (Oreochromis niloticus) exposed to effluents from a swine industry. Arch Environ Contam Toxicol 50:23–30

    Article  CAS  Google Scholar 

  • Lu Z, Min H, Xia Y (2004) The response of Escherichia coli, Bacillus subtilis, and Burkholderia cepacia WZ1 to oxidative stress of exposure to quinclorac. J Environ Sci Health B 39:431–441

    Article  CAS  Google Scholar 

  • Maltby L, Crane M (1994) Responses of Gammarus pulex (Amphipoda, Crustacea) to metalliferous effluents: identification of toxic components and the importance of interpopulation variation. Environ Pollut 84:45–52

    Article  CAS  Google Scholar 

  • Mohamed MS (2009) Degradation of methomyl by the novel bacterial strain Stenotrophomonas maltophilia M1. EJB 12(4):1–6

    Google Scholar 

  • Monteiro DA, de Almeida JA, Rantin FT, Kalinin AL (2006) Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 143(2):141–149

    Article  CAS  Google Scholar 

  • Motoyama N, Dauterman WC (1980) Glutathione S-transferases: their role in the metabolism of insecticides. Rev Biochem Toxicol 2:49–70

    CAS  Google Scholar 

  • Mutch E, Daly AK, Williams FM (2007) The relationship between PON1 phenotype and PON1–192 genotype in detoxification of three oxons by human liver. Drug Metab Dispos 35:315–320

    Article  CAS  Google Scholar 

  • Oncu M, Gultekin F, Karaoz E, Altuntas I, Deliba N (2002) Nephrotoxicity in rats induced by chlorpyrifos-ethyl and ameliorating effects of antioxidants. Hum Exp Toxicol 21:223–230

    Article  CAS  Google Scholar 

  • Panda J, Kanjilal T, Das S (2018) Optimized biodegradation of carcinogenic fungicide carbendazim by Bacillus licheniformis JTC-3 from agro-effluent. Biotechnol Res Innov 2:45–57

    Article  Google Scholar 

  • Peixoto F, Alves-Fernandes D, Santos D, Fontainhas Fernandes A (2006) Toxicological effects of oxyfluorfen on oxidative stress enzymes in tilapia Oreochromis niloticus. Pestic Biochem Physiol 85:91–96

    Article  CAS  Google Scholar 

  • Prasad KVSK, Saradhi P, Sharmila P (1999) Concerted action of antioxidant enzymes and curtailed growth under zinc toxicity in Brassica juncea. Environ Exp Bot 42:1–10

    Article  CAS  Google Scholar 

  • Rewitz KF, Styrishave B, Løbner-Olsen A, Andersen O (2006) Marine invertebrate cytochrome P450: emerging insights from vertebrate and insects analogies. Comp Biochem Physiol C Toxicol Pharmacol 143:363–381

    Article  CAS  Google Scholar 

  • Sahinoz E, Aral F, Dogu Z, Koyuncu İ, Yuksekdag O (2019) The protective effects of curcumin on organophosphate insecticide chlorpyrifos-induced oxidative stress and DNA damage in Oncorhynchus mykiss. Turk J Fish Aquat Sci 20(3):185–195

    Google Scholar 

  • Santos TG, Martinez CB (2012) Atrazine promotes biochemical changes and DNA damage in a Neotropical fish species. Chemosphere 89(9):1118–1125

    Article  CAS  Google Scholar 

  • Sayler GS, Hooper SW, Layton AC, King JMH (1990) Catabolic plasmids of environmental and ecological significance. Microb Ecol 19(1):1–20

    Article  CAS  Google Scholar 

  • Serdar O (2019) The effect of dimethoate pesticide on some biochemical biomarkers in pulex. Environ Sci Pol 26(21):21905–21914

    Article  CAS  Google Scholar 

  • Shi F, Jia X, Zhao C, Chen Y (2010) Antioxidant activities of various extracts from Artemisia selengensis Turcz (LuHao). Molecules 15:4934–4946

    Article  CAS  Google Scholar 

  • Song Y, Chen M, Zhou J (2017) Effects of three pesticides on superoxide dismutase and glutathione-S-transferase activities and reproduction of Daphnia magna. Arch Environ Prot 43(1):80–86

    Article  Google Scholar 

  • Tatar S, Yildirim NC, Serdar O, Erguven GO (2020) Can toxicities induced by insecticide methomyl be remediated via soil bacteria Ochrobactrum thiophenivorans and Sphingomonas melonis? Curr Microbiol 77:1301–1307

    Article  CAS  Google Scholar 

  • Timbrell JA, Draper R, Waterfield CJ (1994) Biomarkers in toxicology: new use of some old molecules? Toxicol Ecotoxicol News 1:4–14

    CAS  Google Scholar 

  • World Health Organization (1986) Organophosphorus insecticides: a general introduction. Environmental Health Criteria, vol 63. WHO, Geneva

    Google Scholar 

  • Xing H, Wang X, Sun G, Gao X, Xua S, Wang X (2012) Effects of atrazine and chlorpyrifos on activity and transcription of glutathione S-transferase in common carp (Cyprinus carpio L.). Environ Toxicol Pharmacol 33:233–244

    Article  CAS  Google Scholar 

  • Xu YG, Zhang JL, Li XF, Liu L (2013) Study on chronic toxicity of difenoconazole to Daphnia magna and its effect on GST enzymatic activity. Hubei Agric Sci 52:79–83

    Google Scholar 

  • Yang Y, Cheng JZ, Singhal SS, Saini M, Pandya U, Awasthi S, Awasthi YC (2001) Role of glutathione S-transferases in protection against lipid peroxidation: overexpression of hGSTA2-2 in K562 cells protects against hydrogen peroxide induced apoptosis and inhibits Jnk and caspase 3 activation. J Biol Chem 276:19220–19230

    Article  CAS  Google Scholar 

  • Yildirim NC, Tanyol M, Yildirim N, Serdar O, Tatar S (2018) Biochemical responses of Gammarus pulex to malachite green solutions decolorized by Coriolus versicolor as a biosorbent under batch adsorption conditions optimized with response surface methodology. Ecotoxicol Environ Saf 30(156):41–47

    Article  CAS  Google Scholar 

  • Yildirim NC, Tanyol M, Serdar O, Yildirim N (2019) Gammarus pulex as a model organism to assess the residual toxicity of slaughterhouse wastewater treated by electrocoagulation process. Bull Envıron Contam Toxicol 103:447–452

    Article  CAS  Google Scholar 

  • Zelles L, Adrian P, Bai QY, Stepper K, Adrian MV, Fischer K, Maier A, Ziegler A (1991) Microbial activity measured in soils stored under different temperature and humidity conditions. Soil Biol Biochem 23:955–962

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry and Chemical Processes, Tunceli Vocation School, Munzur University, 62000, Tunceli, Turkey

    Gokhan Onder Erguven & Şule Tatar

  2. Fisheries Faculty, Munzur University, 62000, Tunceli, Turkey

    Osman Serdar

  3. Department of Veterinary Medicine, Pertek Sakine GencVocational School, Laboratorian and Veterinarian Health Pr, Munzur University, 62000, Tunceli, Turkey

    Nuran Cikcikoglu Yildirim

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  1. Gokhan Onder Erguven
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  2. Şule Tatar
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  3. Osman Serdar
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  4. Nuran Cikcikoglu Yildirim
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Contributions

GOE, OS, and NCY conceived and designed the research; GOE and OS studied at the field. GOE performed bioremediation assays. GOE, ST, OS, and NCY carried out the field measurements with laboratory analysis; NCY and ST studied enzymes. All of the authors performed statistical data analysis; GOE, OS, and NCY performed writing, review, and editing; GOE and NCY supervised the research; and all authors provided major input on the manuscript.

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Correspondence to Gokhan Onder Erguven.

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Onder Erguven, G., Tatar, Ş., Serdar, O. et al. Evaluation of the efficiency of chlorpyrifos-ethyl remediation by Methylobacterium radiotolerans and Microbacterium arthrosphaerae using response of some biochemical biomarkers. Environ Sci Pollut Res 28, 2871–2879 (2021). https://doi.org/10.1007/s11356-020-10672-9

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