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Reliable and disposable quantum dot–based electrochemical immunosensor for aflatoxin B1 simplified analysis with automated magneto-controlled pretreatment system

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Abstract

An integrated aflatoxin B1 (AFB1) detection platform with quantum dot (QD)-based electrochemical immunosensor and an automated magneto-controlled pretreatment system was successfully developed. The automated pretreatment system adopts the immunoaffinity magnetic beads (IMB) as the capture probe of AFB1 and QD-labeled AFB1 complete antigen (AFB1-BSA-QDs) as the signal probe. AFB1-BSA-QDs can be easily converted into corresponding metallic cations through acidic treatment, which can be detected electrochemically via anode stripping voltammetry (ASV). Moreover, a disposable screen-printed electrode (SPE) without requiring any further modification is used in the novel electrochemical immunosensor‚ making routine testing feasible. Under optimal conditions, the detectable concentration range of AFB1 was 0.08–800 μg/kg. The metal ion signal associated linearly with the logarithm of AFB1 concentration within the range of 5–240 μg/kg, with a detection limit of 0.05 μg/kg. The spiked recoveries of three different concentrations in four different matrixes ranged from 83.9 to 118.0%, and inter-day relative standard deviations were below 10%. Furthermore, the methodology was validated by analyzing naturally contaminated samples, and results of the novel immunosensor were in good agreement with those of LC-MS/MS, demonstrating the potentiality of the developed method for the monitor of AFB1 in cereals and oils.

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Funding

This research was funded by the Government’s Key Project on International Scientific and Technological Innovation Cooperation (2016YFE0113000), National Natural Science Foundation of China (81803712, 31901806), the National Key R&D Program of China under Grant 2018YFC1602102-1, the Young Elite Scientists Sponsorship Program by CAST (2018QNRC001), and the Fundamental Research Funds for the Academy of National Food and Strategic Reserves Administration (ZX1913, ZX1922).

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  1. Academy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing, 100037, China

    Zhihong Xuan, Hongmei Liu, Jin Ye, Li Li, Wei Tian & Songxue Wang

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  1. Zhihong Xuan
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Correspondence to Jin Ye or Songxue Wang.

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Xuan, Z., Liu, H., Ye, J. et al. Reliable and disposable quantum dot–based electrochemical immunosensor for aflatoxin B1 simplified analysis with automated magneto-controlled pretreatment system. Anal Bioanal Chem 412, 7615–7625 (2020). https://doi.org/10.1007/s00216-020-02897-x

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