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Nontargeted lipidomics of novel human plasma reference materials: hypertriglyceridemic, diabetic, and African-American

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Abstract

The unavailability of appropriate quality assurance/quality control materials in many lipidomics applications poses a significant challenge for lipidomics research. It is recommended that samples with certified values and/or consensus estimates, such as NIST SRM 1950–Metabolites in Frozen Human Plasma, be implemented in routine analyses to enable community-wide comparisons of lipidomics results and analytical workflows. Herein, we applied a nontargeted lipidomics method for the analysis of a new human plasma reference material suite developed by NIST (hypertriglyceridemic, diabetic, and African-American plasma pools), in addition to SRM 1950. We identified specific lipidomics fingerprints associated with each sample type, including lauric acid–containing lipids and elevated triacylglycerol levels in hypertriglyceridemic plasma, palmitoleic acid–containing lipids in diabetic plasma, and oxidized fatty acid–containing phospholipids in African-American plasma. This work highlights the importance of developing and profiling application-specific reference materials, while establishing reference data that may be used for system suitability and/or quality control metrics.

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Data availability

The datasets collected and analyzed in the present study are available from the corresponding author on reasonable request. A table of the normalized semi-quantitative concentrations for all lipids identified in this study will be provided in the ESM. Experiment files have been deposited in an online repository, and may be downloaded from https://doi.org/10.25345/C5FM7D

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Acknowledgments

The authors would like to acknowledge Tracy Schock, Dan Bearden, and Yamil Simon for their help with material design and procurement, as well as the University of Florida College of Veterinary Medicine for funding support.

Funding

Startup funds from the University of Florida College of Veterinary Medicine were used to support this work.

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Authors and Affiliations

  1. Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, 1333 Center Drive, Gainesville, FL, 32610, USA

    Juan J. Aristizabal-Henao & John A. Bowden

  2. Chemical Sciences Division, National Institutes of Standards and Technology, Gaithersburg, MD, 20899, USA

    Christina M. Jones & Katrice A. Lippa

Authors
  1. Juan J. Aristizabal-Henao
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  2. Christina M. Jones
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  3. Katrice A. Lippa
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  4. John A. Bowden
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Contributions

All authors contributed to the study conception and design. Material preparation was completed by Christina M. Jones and Katrice A. Lippa. Data collection and analysis was performed by Juan J. Aristizabal-Henao. The first draft of the manuscript was written by Juan J. Aristizabal-Henao and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to John A. Bowden.

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The authors declare that they have no conflict of interest.

Ethics approval

All National Institute of Standards and Technology (NIST) plasma samples were collected after informed consent under approved Institutional Review Board protocols reviewed by the NIST Human Subjects Protection Office.

Disclaimer

Certain commercial equipment, instruments, or materials are identified in this paper to adequately specify the experimental procedures. Such identification does not imply recommendation or endorsement by the National Institute of Standards and Technology nor does it imply that the materials or equipment identified are necessarily the best for the purpose. Furthermore, the content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Standards and Technology.

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Aristizabal-Henao, J.J., Jones, C.M., Lippa, K.A. et al. Nontargeted lipidomics of novel human plasma reference materials: hypertriglyceridemic, diabetic, and African-American. Anal Bioanal Chem 412, 7373–7380 (2020). https://doi.org/10.1007/s00216-020-02910-3

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  • DOI: https://doi.org/10.1007/s00216-020-02910-3

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