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Circular RNA has_circ_0000034 accelerates retinoblastoma advancement through the miR-361-3p/ADAM19 axis

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A Correction to this article was published on 21 January 2022

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Abstract

Retinoblastoma (RB) is an intraocular malignancy that mainly occurs in infants and young children under 5 years of age. Circular RNA hsa_circ_0000034 (circ_0000034) was reported to be upregulated in RB tissues. Nevertheless, the function and mechanism of circ_0000034 in RB are unclear. Expression of circ_0000034, microRNA-361-3p (miR-361-3p), and a disintegrin and metalloproteinase 19 (ADAM19) was examined via quantitative real-time polymerase chain reaction (qRT-PCR). Cell viability, migration, invasion, and apoptosis were determined though Cell Counting Kit-8 (CCK-8), transwell, or flow cytometry assays. Caspase-3 activity was detected using a caspase-3 activity assay kit. Some protein levels were examined using Western blot analysis. Dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay, or RNA pull-down assay were performed to verify the relationship between circ_0000034 or ADAM19 and miR-361-3p. The function of circ_0000034 in vivo was confirmed via animal experiment. We verified that circ_0000034 expression was elevated in RB tissues and cells. Circ_0000034 silencing reduced RB growth in vivo, repressed viability, migration, invasion, and EMT, and induced apoptosis of RB cells in vitro. Circ_0000034 acted as a sponge for miR-361-3p, which targeted ADAM19 in RB cells. Furthermore, the inhibition of miR-361-3p restored circ_0000034 knockdown-mediated impacts on viability, migration, invasion, apoptosis, and EMT of RB cells. Moreover, ADAM19 overexpression abolished the influence of miR-361-3p mimic on viability, migration, invasion, apoptosis, and EMT of RB cells. Circ_0000034 expedited RB progression through upregulating ADAM19 via sponging miR-361-3p, which indicated that circ_0000034 might a target for RB therapy.

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  1. Department of Ophthalmology, The Fourth People’s Hospital of Shenyang, No. 20 Huanghe South Street, Huanggu District, Shenyang, 110031, Liaoning, China

    Yanhua Jiang, Fan Xiao, Lin Wang, Ting Wang & Linlin Chen

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Supplementary file1 (TIF 275 kb)

Supplementary Fig.1 Influence of circ_0000034 inhibition on the predicted 10 miRNA levels in RB cells. (A and B) QRT-PCR revealed the levels of miR-1184, miR-1204, miR-1205, miR-361-3p, miR-450b-3p, miR-769-3p, miR-1270, miR-620, miR-638, and miR-873 in Y79 and Weri-Rb1 cells transfected with sh-circ#1 or sh-NC. *P < 0.05.

Supplementary file2 (TIF 281 kb)

Supplementary Fig.2 Influence of miR-361-3p overexpression on the predicted 10 mRNA levels in RB cells. (A and B) QRT-PCR revealed the levels of E2F2, JAK1, NOTCH2, ETV3, FZD4, CCND2, ADAM19, AKT2, MAPK4, ADAM10 mRNA in Y79 and Weri-Rb1 cells transfected with miR-361-3p or miR-NC. *P < 0.05.

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Jiang, Y., Xiao, F., Wang, L. et al. Circular RNA has_circ_0000034 accelerates retinoblastoma advancement through the miR-361-3p/ADAM19 axis. Mol Cell Biochem 476, 69–80 (2021). https://doi.org/10.1007/s11010-020-03886-5

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