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Eosin Y as a high-efficient photooxidase mimic for colorimetric detection of sodium azide

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Abstract

The reported fluorescent dye–based artificial light–responsive oxidase mimics are suffering from their low catalytic efficiency. To overcome the limitation, we report the photooxidase-mimicking activity of Eosin Y which can catalyze the oxidation of various chromogenic substrates such as 3,3′,5,5′-tetramethylbenzydine (TMB), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 3,3′-diaminobenzidine (DAB), and o-phenylenediamine (OPD) by dissolved oxygen. The photooxidase-like activity of Eosin Y is highly efficient for TMB substrate, and its catalytic efficiency is higher than that of the reported fluorescein (130 fold) and 9-mesityl-10-methylacridinium ion (7.7-fold) mimetic photooxidase. Moreover, the photosensitized Eosin Y-TMB chromogenic system is utilized for colorimetric detection of highly toxic and explosive sodium azide (NaN3) in a linear range from 5 to 500 μM with a limit of detection of 3.5 μM. The resulting colorimetric assay is selective and applied to determine NaN3 in real lake water samples.

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Funding

This work was financially supported by the Research Program of State Key Laboratory of NBC Protection for Civilian (SKLNBC2018-02).

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Authors and Affiliations

  1. National Collaborative Innovation Center for Nuclear Waste and Environmental Safety, School of National Defence Science & Technology, Southwest University of Science and Technology, Mianyang, 621010, China

    Junren Wang, Haili Yu & Yi He

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  1. Junren Wang
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  2. Haili Yu
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  3. Yi He
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Correspondence to Haili Yu or Yi He.

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Wang, J., Yu, H. & He, Y. Eosin Y as a high-efficient photooxidase mimic for colorimetric detection of sodium azide. Anal Bioanal Chem 412, 7595–7602 (2020). https://doi.org/10.1007/s00216-020-02895-z

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  • DOI: https://doi.org/10.1007/s00216-020-02895-z

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