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Molecular characterization of a novel fusarivirus infecting the edible fungus Auricularia heimuer

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Abstract

Here, we describe a novel mycovirus, Auricularia heimuer fusarivirus 1 (AhFV1), isolated from the edible fungus Auricularia heimuer strain CCMJ1296. The virus has a single-stranded positive-sense [+ssRNA] genome of 7,127 nucleotides containing two overlapping open reading frames (ORFs) and a poly(A) tail. The large ORF1 encodes a polyprotein of 1,637 amino acids (aa) with conserved RNA-dependent RNA polymerase (RdRp) and DEAD-like helicase superfamily (DEXDc) domains. ORF2 encodes a putative 633-aa protein with unknown function. A BLAST search showed that the nucleotide sequence of the AhFV1 genome is 41.28% identical to that of Sclerotium rolfsii fusarivirus 2 and 40.49% identical to that of Sclerotium rolfsii fusarivirus 1. Phylogenetic analysis based on RdRp and helicase (Hel) sequences indicated that AhFV1 is related to unclassified mycoviruses and other fusariviruses. Our data suggest that AhFV1 should be classified as a member of the newly proposed family “Fusariviridae”. This is the second virus and the first full genome sequence of a fusarivirus from A. heimuer.

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Acknowledgments

We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

Funding

The National Key Research and Development Program of China (2018YFD1001000, 2017YFD0601002), the Special Fund for Agro-Scientific Research in the Public Interest (201503137), the Jilin Provincial Department of Education (JJKH20180670KJ), and the Program of Introducing Talents of Discipline to Universities (D17014) supported this study.

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Authors and Affiliations

  1. Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Xuefei Li, Kunpeng Sui, Weiqi Yin, Frederick Leo Sossah, Bing Song & Yu Li

  2. College of Plant Protection, Jilin Agricultural University, Changchun, 130118, Jilin, China

    Xuefei Li, Kunpeng Sui & Weiqi Yin

  3. State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Jiatao Xie, Du Hai & Daohong Jiang

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  1. Xuefei Li
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  2. Kunpeng Sui
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  4. Du Hai
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Correspondence to Bing Song or Yu Li.

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705_2020_4781_MOESM1_ESM.png

Fig. S1 Comparison of the conserved RdRp amino acid motif of AhFV1 with those of representative viruses of the proposed family “Fusariviridae”

705_2020_4781_MOESM2_ESM.png

Fig. S2 Comparison of the conserved Hel amino acid motif of AhFV1 with those of representative viruses of the proposed family “Fusariviridae”

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Li, X., Sui, K., Xie, J. et al. Molecular characterization of a novel fusarivirus infecting the edible fungus Auricularia heimuer. Arch Virol 165, 2689–2693 (2020). https://doi.org/10.1007/s00705-020-04781-6

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  • DOI: https://doi.org/10.1007/s00705-020-04781-6

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