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Free and Substrate-Immobilised Lipases from Fusarium verticillioides P24 as a Biocatalyst for Hydrolysis and Transesterification Reactions

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Abstract

Fungal enzymes are widely used in technological processes and have some interesting features to be applied in a variety of biosynthetic courses. Here, free and substrate-immobilised lipases from Fusarium verticillioides P24 were obtained by solid-state fermentation using wheat bran as substrate and fungal carrier. Based on their hydrolytic and transesterification activities, the lipases were characterised as pH-dependent in both reactions, with higher substrate conversion in an alkaline environment. Thermally, the lipases performed well from 30 to 45 °C, being more stable in mild conditions. Organic solvents significantly influenced the lipase selectivity using different vegetable oils as fatty acid source. Omega(ω)-3 production in n-hexane achieved 45% using canola oil, against ≈ 18% in cyclohexane. However, ω-6 production was preferably produced for both solvents using linseed oil with significant alterations in the yield (≈ 79% and 49% for n-hexane and cyclohexane, respectively). Moreover, the greatest enzyme selectivity for ω-6 led us to suppose a lipase preference for the Sn1 position of the triacylglycerol. Lastly, a transesterification reaction was performed, achieving 90% of ester conversion in 72 h. This study reports the characterisation and use of free and substrate-immobilised lipases from Fusarium verticillioides P24 as an economic and efficient method for the first time.

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Funding

This study received financial support from the São Paulo Research Foundation (FAPESP Grants 2010/03555-5 and 2008/58077-0) and the National Council for Scientific and Technological Development (CNPq).

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  1. Janaina Pires Borges and José Carlos Quilles Junior contributed equally for the paper preparation

Authors and Affiliations

  1. Departament of Biochemistry and Chemical Technology, IQ/UNESP, Rua Prof. Francisco Degni, 55, CEP, Araraquara, SP, 14800-060, Brazil

    Janaina Pires Borges & Daniela Alonso Bocchini

  2. Department of Chemistry and Environmental Sciences, IBILCE/UNESP, Rua Cristóvão Colombo, 2265, CEP, São José do Rio Preto, SP, 15054-000, Brazil

    José Carlos Quilles Junior, Thiago Hideyuki Kobe Ohe, Mauricio Boscolo & Roberto da Silva

  3. Department of Biology, IBILCE/UNESP, Rua Cristóvão Colombo, 2265, CEP, São José do Rio Preto, SP, 15054-000, Brazil

    Ana Lucia Ferrarezi, Christiane da Costa Carreira Nunes & Eleni Gomes

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  1. Janaina Pires Borges
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Borges, J.P., Quilles Junior, J.C., Ohe, T.H.K. et al. Free and Substrate-Immobilised Lipases from Fusarium verticillioides P24 as a Biocatalyst for Hydrolysis and Transesterification Reactions. Appl Biochem Biotechnol 193, 33–51 (2021). https://doi.org/10.1007/s12010-020-03411-w

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