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The expression of bitter taste receptor TAS2R38 in patients with chronic rhinosinusitis

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Archivum Immunologiae et Therapiae Experimentalis Aims and scope

Abstract

Chronic rhinosinusitis (CRS) is a frequent disease with high social impact and multifactorial pathogenesis. Recently, the bitter taste receptor TAS2R38 has been described to play a role in upper airway innate mucosal defense. The aim was to determine the localization and expression of the TAS2R38 in the selected cell lines and tissue collected from patient suffered from CRS as well as to correlate the results with clinical data. Moreover, the purpose was the estimation of the TAS2R38 distribution changes during acute and CRS. Forty-two patients undergoing nasal surgery were enrolled in the study. The TAS2R38 expression was assessed in the collected tissues using immunohistochemistry and immunocytochemistry methods. The western blot analysis was performed on human cell lines HeLa, MCF7, MDA-MB-231 to assess the location of the TAS2R38 protein. Moreover, the HeLa cell line was used as a model of acute inflammation induces by lipopolysaccharide. Immunohistochemistry analysis displayed a statistically significant difference of TAS2R38 level in the patients with CRS compared to healthy control and was different in CRS with and without nasal polyps. The results showed the abundance of TAS2R38 receptor in the cell nucleus in patients with CRS and cell lines. The variance in TAS2R38 receptor expression in two CRS types suggests their different pathogenesis. The first time in literature, we confirmed the presence of plasma membrane TAS2R38 receptor in the cell nuclei in CRS as well as in cell lines, what strongly suggests the different than membrane TAS2R38 function.

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Abbreviations

CRS:

Chronic rhinosinusitis

CRSwNP:

Chronic rhinosinusitis with nasal polyps

CRSsNP:

Chronic rhinosinusitis sine nasal polyps

EPOS:

European position paper on rhinosinusitis

IHC:

Immunohistochemistry

ICC:

Immunocytochemistry

LPS:

Lipopolysaccharide

SNPs:

Single-nucleotide polymorphisms

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Acknowledgements

We thank the patients for participating in this study.

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Author notes

  1. Karolina Zborowska-Piskadło and Małgorzata Stachowiak contributed equally to this work.

Authors and Affiliations

  1. Department of Otolaryngology, Miedzyleski Hospital, Warsaw, Poland

    Karolina Zborowska-Piskadło & Karolina Dżaman

  2. Department of Molecular and Translational Oncology, Maria Sklodowska-Curie Institute-Oncology Center, Warsaw, Poland

    Małgorzata Stachowiak, Natalia Rusetska, Elżbieta Sarnowska & Janusz Siedlecki

  3. Department of Otolaryngology, Centre of Postgraduate Medical Education, Kondratowicza 8, 03-242, Warsaw, Poland

    Karolina Dżaman

Authors
  1. Karolina Zborowska-Piskadło
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  2. Małgorzata Stachowiak
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  3. Natalia Rusetska
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  4. Elżbieta Sarnowska
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  5. Janusz Siedlecki
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  6. Karolina Dżaman
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Contributions

Conceived and designed the experiments: KZP, MS, ES, JS, and KD. Performed the experiments: KZP, MS, and NR. Analyzed the data: KZP, MS, ES, and KD. Contributed reagents/materials/analysis tools: KZP, MS, and JS. Wrote the paper: KZP, MS, ES, KD.

Corresponding author

Correspondence to Karolina Dżaman.

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Informed consent was obtained from all individual participants included in the study.

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The authors declare that they have no conflict of interest.

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Zborowska-Piskadło, K., Stachowiak, M., Rusetska, N. et al. The expression of bitter taste receptor TAS2R38 in patients with chronic rhinosinusitis. Arch. Immunol. Ther. Exp. 68, 26 (2020). https://doi.org/10.1007/s00005-020-00593-3

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  • DOI: https://doi.org/10.1007/s00005-020-00593-3

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