Abstract
Bt cotton plants, with bollworm resistant trait, have been widely adopted since introduced in India in 2002. As a proactive measure to prevent evolution of resistance to Bt protein(s), it was approved for commercial cultivation with a stipulation of structured refuge of minimum 20% of the Bt area, constituted by plants that do not produce Bt toxins and thus allow growth and development of population of Bt susceptible pests. Though the refuge cotton seeds are provided in a separate pouch within each Bt cotton seed packet, Indian farmers have been reluctant to sow refuge. To find out a method for ensured refuge planting, we experimented with ‘built-in-refuge’ (BIR) in which non-Bt cotton seeds were blended with Bt cotton seeds in various proportions ie 5,10, 50% along with 20% recommended structured refuge and each treatment was evaluated in terms of fruiting body damage by major cotton lepidopterans, relative to the regulator stipulated 20% non-Bt structured refuge at five locations during cotton growing season of 2012–13. A major outcome from this study was that treatments with 5 and 10% non-Bt blends with bollga0rd-II were as effective as the 20% structured refuge, based on fruiting body damage due to bollworms, a key factor in managing Bt resistance without any compromise to the seed cotton yield. It can be concluded that the seed blend technology is a good option to impose refugia compliance to delay resistance in Bt cotton to bollworms in a country having lesser alternate hosts available for the target bollworms during the season and voluntary compliance of refuge is absent.
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Acknowledgements
This research was funded by Indian Council of Agricultural Research (ICAR), New Delhi through Public Private Partnership project with Mahyco Monsanto Biotech Ltd. The authors are also highly thankful to the Director, ICAR-Central Institute for Cotton Research and Head, ICAR-CICR, Regional Station, Sirsa for providing the necessary facilities.
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Kumar, R., Kranthi, S., Rao, G.M.V.P. et al. Assessment of bollworm damage and yield loss in seed blends of Bollgard-II with corresponding Non-Bt hybrid as ‘built in refuge’ in cotton. Phytoparasitica 49, 253–263 (2021). https://doi.org/10.1007/s12600-020-00846-z
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DOI: https://doi.org/10.1007/s12600-020-00846-z