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Analysis of clinical important of LncRNA-HOTAIR gene variations and ovarian cancer susceptibility

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Abstract

LncRNAs are of functional long non-coding RNAs, which have been shown to be involved in critical pathways in cancer development. LncRNA-HOTAIR gene overexpression has been reported in several cancers. The aim of this study was to evaluate the associations between two variants of lncRNA-HOTAIR (rs1899663 G>T and rs4759314 A>G) gene polymorphisms and the risk of ovarian cancer (OC) susceptibility. We performed a case and control analysis on two hundred individuals consisting of 100 cases with OC and 100 women cancer-free in East Azerbaijan of Iranian population. To evaluate the association between two SNPs of lncRNA-HOTAIR with the risk of OC susceptibility used the polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) method. We revealed that two SNPs in the lncRNA-HOTAIR gene were significantly associated with the risk of OC. The dominant model of rs4759314 in lncRNA-HOTAIR (AA vs. GA/GG) showed a significantly increased risk with an OR of 10.036 (CI 2.253–44.712, P = 0.000); the recessive model of rs1899663 (TT vs. GT/GG) revealed a significantly increased risk with OR of 0.910 (CI 0.856–0.968; P = 0.002). In addition, our findings demonstrated that the 4759314G (OR 13.500; CI 3.146–57.940; P = 0.000) and 1899663T (OR 3.273; CI 1.433–7.475; P = 0.003) alleles are increased the risk of OC susceptibility. Our findings provide evidence that the specific genetic variants in lncRNA-HOTAIR gene may affect OC susceptibility in an Iranian population.

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Acknowledgements

The current investigation was extracted from of MS.c (IR: 952003), which was confirmed at the Ahar Branch, Islamic Azad University, Iran. The authors would like to thank all of the subjects who participant in our survey.

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The present survey has no any fund.

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  1. Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran

    Narmella Saeedi & Saeid Ghorbian

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  1. Narmella Saeedi
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  2. Saeid Ghorbian
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Correspondence to Saeid Ghorbian.

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Saeedi, N., Ghorbian, S. Analysis of clinical important of LncRNA-HOTAIR gene variations and ovarian cancer susceptibility. Mol Biol Rep 47, 7421–7427 (2020). https://doi.org/10.1007/s11033-020-05797-6

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