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Capture and toxicity assessment of Ag citrate nanoparticles using jellyfish extract

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Abstract

Backgrounds

In this study, Jellyfish extract at immunity reaction (JEI) captured Ag citrate nanoparticles (Ag citrate NPs) and reduce toxicity was investigated.

Methods

JEI obtained from Aurelia aurita was used to assess the capture of Ag citrate NPs (10 mg/L, 2 mg/L) during 24 h, and the toxicity of JEI was assessed using a human cell line BEAS-2B.

Results

JEI was to reduce Ag citrate NPs in water by 34.55% (10 mg/L) after 1 h and by 40.62 ~ 41% (2 mg/L, 10 mg/L) after 24 h. When the BEAS-2B was exposed to 2 mg/L of Ag citrate NPs mixed with JEI showed increase 33.97% survival than 2 mg/L of Ag citrate NPs (0%). Furthermore, the apoptosis-related genes (p53, Bcl2, and Bax) showed no significant difference in expression upon exposure to JEI, compared to the control.

Conclusion

JEI is to capture Ag citrate NPs and reduce its nano-toxicity. Therefore, such ability of JEI is likely to prove useful in the control of AgNPs flowing into the aquatic ecosystem.

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Acknowledgements

This study was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF), which is funded by the Ministry of Education (NRF-2016R1A2B4016442 and NRF-2019R1A2C1004616).

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Authors and Affiliations

Authors

Contributions

MKY designed the research; JH, EK, and BGL performed the research; MKY and JH analyzed the data; MKY and JH wrote the paper.

Corresponding author

Correspondence to Min-Kyeong Yeo.

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Conflict of interest

Jiwon Ha, Euna Kim, Bong Gu Lee, and Min-Kyeong Yeo declare that they have no conflicts of interest.

Ethical approval

The study was reviewed and approved by the Research Ethics Committee of Kyung Hee University. Informed consent statement: all study participants, or their legal guardian, provided informed written consent prior to study enrollment.

Human and animal rights

The experimental procedures followed the law of animal protection that was approved by the Animal Care Advisory Committee of Kyung Hee University, Republic of Korea.

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Ha, J., Kim, E., Lee, B.G. et al. Capture and toxicity assessment of Ag citrate nanoparticles using jellyfish extract. Mol. Cell. Toxicol. 16, 431–439 (2020). https://doi.org/10.1007/s13273-020-00100-x

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