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Molecular and genome characterization of colistin-resistant Escherichia coli isolates from wild sea lions (Zalophus californianus)

  • Environmental Microbiology - Short Communication
  • Published:
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Abstract

Using molecular and whole-genome sequencing tools, we investigated colistin-resistant Escherichia coli isolates from wild sea lions. Two unrelated E. coli colistin-resistant isolates, ST8259 and ST4218, were identified, both belonging to the B2 phylogroup and different serotypes. Polymorphisms in PmrA, PmrB, and PhoQ proteins were identified, and the role of PmrB and PhoQ in contributing to colistin resistance was determined by complementation assays. However, the mutations characterized in the present study are not involved in colistin resistance, which have been described in E. coli isolates from clinical settings. Therefore, the acquired mutations in pmrB and phoQ genes in resistance to colistin in bacteria related to marine environment animals are different. This work contributes to the surveillance and characterization of colistin resistance in Escherichia coli obtained from animals from aquatic environments.

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Fig. 1

Data availability

The annotated genome sequences are available at the European Nucleotide Archive under the following accession numbers: CAACXL010000001-CAACXL010000288 (https://www.ncbi.nlm.nih.gov/nuccore/CAACXL000000000.1) for E. coli 13591 and CAACXM010000001-CAACXM010000241 (https://www.ncbi.nlm.nih.gov/nuccore/CAACXM000000000.1) for E. coli 13645.

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Acknowledgments

We thank Dr. Michael Dunn from the Center for Genomic Science-UNAM for reviewing the manuscript.

Funding

This work was supported by grant 256988 from SEP-CONACyT (Secretaría de Educación Pública-Consejo Nacional de Ciencia y Tecnología).

Author information

Author notes

  1. Rigoberto Hernández-Castro and Jonathan Rodríguez-Santiago contributed equally to this work.

Authors and Affiliations

  1. Departamento Ecología de Agentes Patógenos, Hospital General “Dr. Manuel Gea González”, Ciudad de México, Mexico

    Rigoberto Hernández-Castro

  2. Laboratorio de Resistencia Bacteriana; Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Instituto Nacional de Salud Pública (INSP), Cuernavaca, Morelos, Mexico

    Jonathan Rodríguez-Santiago, Jesús Silva-Sánchez, Alejandro Sánchez-Pérez & Ulises Garza-Ramos

  3. Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Instituto Nacional de Salud Pública (INSP), Cuernavaca, Morelos, Mexico

    Juan Téllez-Sosa & Sugey Bravo-Romero

  4. Área de Protección de Flora y Fauna. Islas del Golfo de California, Baja California, CONANP, SEMARNAT. Bahía de Los Ángeles, 22980, Ensenada, Baja California, Mexico

    Rosalia Avalos-Téllez

  5. Departamento de Patología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Coyoacán, 04510, Ciudad de México, Mexico

    Luary Carolina Martínez-Chavarría

  6. Laboratorio de Bacteriología Intestinal, Hospital Infantil de México Dr. Federico Gómez, Cuauhtémoc, 06720, Ciudad de México, Mexico

    Juan Xicohtencatl-Cortes

Authors
  1. Rigoberto Hernández-Castro
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  2. Jonathan Rodríguez-Santiago
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  3. Juan Téllez-Sosa
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  7. Rosalia Avalos-Téllez
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  10. Ulises Garza-Ramos
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Corresponding author

Correspondence to Ulises Garza-Ramos.

Ethics declarations

The study protocol was approved by Secretaries of Environment and Natural Resources (SEMARNAT; SGPA/DGVS/0201211) and of the Interior (SEGOB, SATI/PC/006/111). The study was approved by the Ethics Committee on Research from the Instituto Nacional de Salud Publica.

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The authors declare that they have no conflict of interest.

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Responsible Editor: Nilton Lincopan.

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Hernández-Castro, R., Rodríguez-Santiago, J., Téllez-Sosa, J. et al. Molecular and genome characterization of colistin-resistant Escherichia coli isolates from wild sea lions (Zalophus californianus). Braz J Microbiol 51, 2009–2014 (2020). https://doi.org/10.1007/s42770-020-00371-5

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