Abstract
In the current work, the composite coating of natural hydroxyapatite (nHA)/single-walled carbon nanotubes (SWCNTs) with various contents of SWCNTs (0, 0.5, 1, and 2 wt.%) was applied on NiTi using electrophoretic deposition (EPD). Before the deposition process, the nanotubes were functionalized following the chemical oxidation method and characterized by FTIR. The sintering of samples was conducted at 800 °C for 3 h in a tube furnace under an argon atmosphere. The surface and cross-sectional microstructure of coatings was studied using a scanning electron microscope. The x-ray diffraction was utilized to investigate the effect of the SWCNTs secondary phase on the phase composition of the nHA layer after sintering. The incorporation of SWCNTs into the nHA layer resulted in the decomposition of some HA into the β-tricalcium phosphate phase. The tensile test was applied and displayed the enhancement in adhesion strength of nHA coating from 17.2 to 25 MPa after composing with 2 wt.% SWCNTs. The wettability of surfaces was assessed by measuring DMEM cell culture contact angles. The appraisement of the Ni ions release from the substrate demonstrated that the lowest release of Ni ions into DMEM cell culture after 7 days of incubation is achieved from NiTi coated with nHA-1 wt.% SWCNTs. The in vitro biocompatibility of the samples was pursued by MG63 osteoblast cell culturing and MTT assay. The highest cell attachment and proliferation on nHA-1 wt.% SWCNTs coating were attributed to the least toxic Ni ions release from the substrate of that.
References
K. Vanmeensel, K. Lietaert, B. Vrancken, S. Dadbakhsh, X. Li, J.P. Kruth, P. Krakhmalev, I. Yadroitsev, J.V. Humbeeck, Additively Manufactured Metals for Medical Applications, Additive Manufacturing: Materials, Processes, Quantifications and Applications, 1st ed., J. Zhang, Y.G. Jung, Ed., Butterworth-Heinemann, 2018, p 261–309
M.A. Baumann, Nickel-Titanium: Options and Challenges, J. Dent. Clin. North. Am., 2004, 48, p 55–67
B. Priyadarshini, M. Rama, and U. Vijayalakshmi, Bioactive Coating as a Surface Modification Technique for Biocompatible Metallic Implants: A Review, J. Asian Ceram. Soc., 2019, 7(4), p 397–406
X. Wang, F. Liu, Y. Song, and Q. Sun, Enhanced Corrosion Resistance and Bio-Performance of Al2O3 Coated NiTi Alloy Improved by Polydopamine-Induced Hydroxyapatite Mineralization, J. Surf. Coat. Technol., 2019, 364, p 81–88
J. Ryhänen, M. Kallioinen, J. Tuukkanen, J. Junila, E. Niemelä, P. Sandvik, and W. Serlo, In Vivo Biocompatibility Evaluation of Nickel-Titanium Shape Memory Metal Alloy: Muscle and Perineural Tissue Responses and Encapsule Membrane Thickness, J. Biomed. Mater. Res. B Appl. Biomater., 1998, 41(3), p 481–488
V. Sansone, D. Pagani, and M. Melato, The Effects on Bone Cells of Metal Ions Released From Orthopaedic Implants, J. Clin. Cases Miner. Bone Metab., 2013, 10(1), p 34–40
N. Horandghadim, J. Khalil-Allafi, and M. Urgen, Effect of Ta2O5 Content on the Osseointegration and Cytotoxicity Behaviors in Hydroxyapatite-Ta2O5 Coatings Applied by EPD on Superelastic NiTi Alloys, J. Mater. Sci. Eng. C, 2019, 102, p 683–695
Y. Say and B. Aksakal, Silver/Selenium/Chitosan-Doped Hydroxyapatite Coatings on Biological NiTi Alloy: In Vitro Biodegradation Analysis, J. Solgel. Sci. Technol., 2019, 90, p 434–442
L. Meng, Y. Wu, K. Pan, Y. Zhu, X. Li, W. Wei, and X. Liu, Polymeric Nanoparticles-Based Multi-Functional Coatings on NiTi Alloy with Nickel Ion Release Control, Cytocompatibility, and Antibacterial Performance, New J. Chem., 2019, 43, p 1551–1561
W. Suchanek and M. Yoshimura, Processing and Properties of Hydroxyapatite-based Biomaterials for Use as Hard Tissue Replacement Implants, J. Mater. Res., 1998, 13(1), p 94–117
S.O.R. Sheykholeslami, J. Khalil-Allafi, and L. Fathyunes, Preparation, Characterization, and Corrosion Behavior of Calcium Phosphate Coating Electrodeposited on the Modified Nanoporous Surface of NiTi Alloy for Biomedical Applications, J. Metal. Mater. Trans. A., 2018, 49, p 5878–5887
T. Sattar, T. Manzoor, F.A. Khalid, M. Akmal, and Gh Saeed, Improved In Vitro Bioactivity and Electrochemical Behavior of Hydroxyapatite-Coated NiTi Shape Memory Alloy, J. Mater. Sci., 2019, 54, p 7300–7306
A. Karimzadeh, M.R. Ayatollahi, A.R. Bushroa, and M.K. Herliansyah, Effect of Sintering Temperature on Mechanical and Tribological Properties of Hydroxyapatite Measured by Nanoindentation and Nanoscratch Experiments, J. Ceram. Int., 2014, 40, p 9159–9164
J. Guillem-Marti, N. Cinca, M. Punset, I.G. Cano, F.J. Gil, J.M. Guilemany, and S. Dosta, Porous Titanium-Hydroxyapatite Composite Coating Obtained on Titanium by Cold Gas Spray with High Bond Strength for Biomedical Applications, J. Colloids Surf B Biointerfaces, 2019, 180, p 245–253
N. Horandghadim, J. Khalil-Allafi, and M. Urgen, Influence of Tantalum Pentoxide Secondary Phase on Surface Features and Mechanical Properties of Hydroxyapatite Coating on NiTi Alloy Produced by Electrophoretic Deposition, J. Surf. Coat. Technol., 2020, 386, p 125458
O. Geuli, I. Lewinstein, and D. Mandler, Composition-Tailoring of ZnO-Hydroxyapatite Nanocomposite as Bioactive and Antibacterial Coating, J. ACS Appl. Nano Mater., 2019, 2(5), p 2946–2957
H. Wang, X. Tian, and X. Ren, Influence of Yttria Stabilized Zirconia and Hydrothermal Treatment on Plasma Sprayed Hydroxyapatite Coatings, J. Wuhan Univ. Technol.-Math. Sci. Edit., 2020, 35, p 449–454
M. Li, Q. Liu, Zh Jia, X. Xu, Y. Cheng, Y. Zheng, T. Xi, and Sh Wei, Graphene Oxide/Hydroxyapatite Composite Coatings Fabricated by Electrophoretic Nanotechnology for Biological Applications, Carbon, 2014, 67, p 185–197
B. Majkowska-Marzec, D. Rogala-Wielgus, M. Bartmanski, B. Bartosewicz, and A. Zielinski, Comparison of Properties of the Hybrid and Bilayer MWCNTs-Hydroxyapatite Coatings on Ti Alloy, Coatings, 2019, 9, p 643
B. Li, X. Xia, M. Guo, Y. Jiang, Y. Li, Zh Zhang, Sh Liu, H. Li, Ch Liang, and H. Wang, Biological and Antibacterial Properties of the Micro-Nanostructured Hydroxyapatite/Chitosan Coating on Titanium, J. Sci. Rep., 2019, 9, p 14052
W. Soutter, Ceramic Matrix Nanocomposites with Carbon Nanotubes, AZoNano. 21 July 2020. https://www.azonano.com/article.aspx?ArticleID=3168.
Y. Chen, Y.Q. Zhang, T.H. Zhang, C.H. Gan, C.Y. Zheng, and G. Yu, Carbon Nanotube Reinforced Hydroxyapatite Composite Coatings Produced Through Laser Surface Alloying, Carbon, 2006, 44(1), p 37–45
X. Li, X. Liu, J. Huang, Y. Fan, and F.-Z. Cui, Biomedical Investigation of CNT Based Coatings, J. Surf. Coat. Technol., 2011, 206, p 759–766
R.L. Spear and R.E. Cameron, Carbon Nanotubes for Orthopaedic Implants, Int. J. Mater. Form., 2008, 1(2), p 127–133
Y. Chen, T.H. Zhang, C.H. Gan, and G. Yu, Wear Studies of Hydroxyapatite Composite Coating Reinforced by Carbon Nanotubes, Carbon, 2007, 45, p 998–1004
D. Lahiri, V. Singh, A.K. Keshri, S. Seal, and A. Agarwal, Carbon Nanotube Toughened Hydroxyapatite by Spark Plasma Sintering: Microstructural Evolution and Multiscale Tribological Properties, Carbon, 2010, 48, p 3103–3120
G.D. Zhan, J.D. Kuntz, J. Wan, and A.K. Mukherjee, Single-Wall Carbon Nanotubes as Attractive Toughening Agents in Alumina-Based Nanocomposites, J. Nat. Mater., 2003, 2, p 38–42
X. Pei, Y. Zeng, R. He, Zh Li, L. Tian, J. Wang, Q. Wan, X. Li, and H. Bao, Single-Walled Carbon Nanotubes/Hydroxyapatite Coatings on Titanium Obtained by Electrochemical Deposition, J. Appl. Surf. Sci., 2014, 295, p 71–80
J.E. Park, Y.S. Jang, T.S. Bae, and M.H. Lee, Biocompatibility Characteristics of Titanium Coated with Multi Walled Carbon Nanotubes-Hydroxyapatite Nanocomposites, J. Mater., 2019, 12(2), p 1–12
J. Liu and X. Ji, Hydroxyapatite-Carbon Nanotubes Composite Coatings on Ti Substrate, J. Adv. Mater. Res., 2012, 424–425, p 86–89
A. Abrishamchian, T. Hooshmand, M. Mohammadi, and F. Najafi, Preparation and Characterization of Multi-Walled Carbon Nanotube/Hydroxyapatite Nanocomposite Film Dip Coated on Ti-6Al-4 V by Sol-Gel Method for Biomedical Applications: An In Vitro Study, J. Mater. Sci. Eng. C, 2013, 33, p 2002–2010
B.D. Hahn, J.M. Lee, D.S. Park, J.J. Choi, J. Ryu, W.H. Yoon, B.K. Lee, D.S. Shin, and H.E. Kim, Mechanical and In Vitro Biological Performances of Hydroxyapatite-Carbon Nanotube Composite Coatings Deposited on Ti by Aerosol Deposition, J. Acta Biomater., 2009, 5, p 3205–3214
C. Kaya, I. Singh, and A.R. Boccaccini, Multi-Walled Carbon Nanotube-Reinforced Hydroxyapatite Layers on Ti6Al4V Medical Implants by Electrophoretic Deposition (EPD), J. Adv. Eng. Mater., 2008, 10(1–2), p 131–138
A.R. Boccaccini, J. Cho, T. Subhani, C. Kaya, and F. Kaya, Electrophoretic Deposition of Carbon Nanotube-Ceramic Nanocomposites, J. Eur. Ceram. Soc., 2010, 30, p 1115–1129
Y. Bai, M.P. Neupane, S. Park, M.H. Lee, T.S. Bae, F. Watari, and M. Uo, Electrophoretic Deposition of Carbon Nanotubes-Hydroxyapatite Nanocomposites on Titanium Substrate, J. Mater. Sci. Eng. C, 2010, 30, p 1043–1049
P. Ducheyne, W.V. Raemdonck, J.C. Heughebaert, and M. Heughebaert, Structural Analysis of Hydroxyapatite Coatings on Titanium, J. Biomater., 1986, 7(2), p 97–103
Ch Du, D. Heldbrant, and N. Pan, Preparation and Preliminary Property Study of Carbon Nanotubes Films by Electrophoretic Deposition, J. Mater. Lett., 2002, 57, p 434–438
I. Singh, C. Kaya, M.S.P. Shaffer, B.C. Thomas, and A.R. Boccaccini, Bioactive Ceramic Coatings Containing Carbon Nanotubes on Metallic Substrates by Electrophoretic Deposition, J. Mater. Sci., 2006, 41, p 8144–8151
L. Besra and M. Liub, A Review on Fundamentals and Applications of electrophoretic deposition (EPD), Prog. Mater Sci., 2007, 52(1), p 1–61
P. Sarkar and P.S. Nicholson, Electrophoretic Deposition (EPD): Mechanisms, Kinetics, and Application to Ceramics, J. Am. Ceram. Soc., 1996, 79(8), p 1987–2002
C. Lin, H. Han, F. Zhang, and A. Li, Electrophoretic Deposition of HA/MWNTs Composite Coating for Biomaterial Applications. J. Mater. Sci.: Mater, Med., 2008, 19, p 2569–2574
S. Vardharajula, S.Z. Ali, P.M. Tiwari, E. Eroglu, K. Vig, V.A. Dennis, and ShR Singh, Functionalized Carbon Nanotubes: Biomedical Applications, Int. J. Nanomed., 2012, 7, p 5361–5374
H. Maleki-Ghaleh, V. Khalili, J. Khalil-Allafi, and M. Javidi, Hydroxyapatite Coating on NiTi Shape Memory Alloy by Electrophoretic Deposition Process, J. Surf. Coat. Tech., 2012, 208, p 57–63
A.M. Rashidi, M.M. Akbarnejad, A.A. Khodadadi, Y. Mortazavi, and A. Ahmadpourd, Single-Wall Carbon Nanotubes Synthesized Using Organic Additives to Co-Mo Catalysts Supported on Nanoporous MgO, J. Nanotech., 2007, 18(31), p 315605
S.W. Kim, T. Kim, Y.S. Kim, H.S. Choi, H.J. Lim, S.J. Yang, and ChR Prak, Surface Modifications for the Effective Dispersion of Carbon Nanotubes in Solvents and Polymers, Carbon, 2012, 50, p 3–33
V. Mussi, C. Biale, S. Visentin, N. Barbero, M. Rocchia, and U. Valbusa, Raman Analysis and Mapping for the Determination of COOH Groups on Oxidized Single Walled Carbon Nanotubes, Carbon, 2010, 48, p 3391–3398
H. Kitamura, M. Sekido, H. Takeuchi, and M. Ohno, The Method for Surface Functionalization of Single-Walled Carbon Nanotubes with Fuming Nitric Acid, Carbon, 2011, 49, p 3851–3856
L.N. Suvarapu, S.O. Baek, Recent Studies on the Speciation and Determination of Mercury in Different Environmental Matrices Using Various Analytical Techniques, Int. J. Anal. Chem., 2017, 2017, p 1–28.
J. Chen, Q. Chen, and Q. Ma, Influence of Surface Functionalization via Chemical Oxidation on the properties of carbon nanotubes, J. Colloid Interface Sci., 2012, 370, p 32–38
M. Es-Souni, M. Es-Souni, and H. Fischer-Brandies, Assessing the Biocompatibility of NiTi Shape Memory Alloys Used for Medical Application, J. Anal. Bioanal. Chem., 2005, 381(3), p 557–567
R.W. Nilen and P.W. Richter, The Thermal Stability of Hydroxyapatite in Biphasic Calcium Phosphate Ceramics, J. Mater. Sci. Mater. Med., 2008, 19(4), p 1693–1702
X.Y. Pang and X. Bao, Influence of Temperature, Ripening Time and Calcination on the Morphology and Crystallinity of Hydroxyapatite Nanoparticles, J. Euro. Ceram. Soc., 2003, 23(10), p 1697–1704
C.M. Assis, L.C. Vercik, M.L. Santos, M.V.L. Fook, and A.C. Guastaldi, Comparison of Crystallinity Between Natural Hydroxyapatite and Synthetic Cp-Ti/HA Coatings, J. Mater. Res., 2005, 8(2), p 207–211
L. Stappers, L. Zhang, O. Van der Biest, and J. Fransaer, The Effect of Electrolyte Conductivity on Electrophoretic Deposition, J. Colloid Interface Sci., 2008, 328, p 436
N. Horandghadim and J. Khalil-Allafi, Characterization of Hydroxyapatite-Tantalum Pentoxide Nanocomposite Coating Applied by Electrophoretic Deposition on Nitinol Superelastic Alloy, J. Ceram. Inter., 2019, 45, p 10448–10460
A. Troelstra, Applying Coatings by Electrophoresis, J. Philips Tech. Rev., 1951, 12, p 293–303
V. Khalili, J. Khalil-Allafi, W. Xia, A.B. Parsa, J. Frenzel, Ch Somsen, and G. Eggeler, Preparing Hydroxyapatite-Silicon Composite Suspensions with Homogeneous Distribution of Multi-Walled Carbon Nano-Tubes for Electrophoretic Coating of NiTi Bone Implant and Their Effect on the Surface Morphology, J. Appl. Surf. Sci., 2016, 366, p 158–165
H. Maleki-Ghaleh and J. Khalil-Allafi, Characterization, Mechanical and In Vitro Biological Behavior of Hydroxyapatite-Titanium-Carbon Nanotube Composite Coatings Deposited on NiTi Alloy by Electrophoretic Deposition, J. surf. Coat. Technol., 2019, 363, p 179–190
N. Horandghadim, J. Khalil-Allafi, E. Kaçar, and M. Urgen, Biomechanical Compatibility and Electrochemical Stability of HA/Ta2O5 Nanocomposite Coating Produced by Electrophoretic Deposition on Superelastic NiTi Alloy, J. Alloys Compd., 2019, 799, p 193–204
C. Kaya, F. Kaya, J. Cho, J.A. Roether, and A.R. Boccaccini, Carbon Nanotube-Reinforced Hydroxyapatite Coatings on Metallic Implants Using Electrophoretic Deposition, J. Key Eng. Mater., 2009, 412, p 93–97
ShF Ou, ShY Chiou, and K.L. Ou, Phase Transformation on Hydroxyapatite Decomposition, J. Ceram. Inter., 2013, 39, p 3809–3816
M. Canillas, P. Pena, A.H. de Aza, and M.A. Rodríguez, Calcium Phosphates for Biomedical Applications, J. Bol. Soc. Esp. Cerám. V, 2017, 56(3), p 91–112
R.A. Gittens, L. Scheideler, F. Rupp, and B.D. Boyan, A Review on the Wettability of Dental Implant Surfaces II: Biological and Clinical Aspects, J. Acta Biomater., 2014, 10, p 2907–2918
L. Fathyunes and J. Khalil-Allafi, The Effect of Graphene Oxide on Surface Features, Biological Performance and Bio-Stability of Calcium Phosphate Coating Applied by Pulse Electrochemical Deposition, J. Appl. Surf. Sci., 2018, 437, p 122–135
K. Webb, V. Hlady, and P.A. Tresco, Relationships Among Cell Attachment, Spreading, Cytoskeletal Organization, and Migration Rate for Anchorage-Dependent Cells on Model Surfaces, J. Biomed. Mater. Res. B Appl. Biomater., 2000, 49(3), p 362–368
A. Khandan, M. Abdellahi, N. Ozada, and H. Ghayour, Study of the Bioactivity, Wettability and Hardness Behaviour of the Bovine Hydroxyapatite-Diopside bio-Nanocomposite Coating, J. Taiwan Inst. Chem. Eng., 2016, 60(c), p 538–546
Z. Huan, L.E. Fratila-Apachitei, and J. Duszczyk, Effect of Aging Treatment on the In Vitro Nickel Release from Porous Oxide Layers on NiTi, J. Appl. Surf. Sci., 2013, 274, p 266–272
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Nematzadeh, L., Horandghadim, N., Khalili, V. et al. In Vitro Biological Characterization of Natural Hydroxyapatite/Single-Walled Carbon Nanotube Composite Coatings Synthesized by Electrophoretic Deposition on NiTi Shape Memory Alloy. J. of Materi Eng and Perform 29, 6170–6180 (2020). https://doi.org/10.1007/s11665-020-05094-0
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DOI: https://doi.org/10.1007/s11665-020-05094-0