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Genetic diversity of Pseudocercospora griseola resistance loci in common beans

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Abstract

Common bean (Phaseolus vulgaris L.) is one of the most widely grown legumes in the world. Although the crop has high yield potential, average yields in Brazil are low due to several diseases. Angular leaf spot (ALS), caused by Pseudocercospora griseola, is among the most important diseases. A set of 81 accessions from the Instituto Agronômico (IAC, Campinas, SP) germplasm bank were evaluated for ALS resistance and genotyped by 12 microsatellites previously associated with ALS QTL resistance. Allele frequencies, number of alleles per locus, expected heterozygosity (He), and Shannon’s Information Index (I) were calculated. The average Ho was 0.12, and the He was 0.54. The STRUCTURE analysis and UPGMA clustering based on Nei’s genetic distance indicated a moderate degree of genetic diversity, with 4 and 5 main groups, respectively. Evaluation of the severity of ALS showed that 17% of the accessions had resistance. Cultivars were recommended for breeding crosses aimed at gaining in genetic diversity and resistance to ALS.

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Acknowledgments

We are very thankful to Dr. Elaine Aparecida de Souza from the Genetics and Plant Breeding Department (UFLA, MG, Brazil) who gently provided the P. griseola isolate for the development of this study.

Funding

We are grateful to the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for granting funding for the research project (FAPESP Number 2014/11145-2) and scholarships (FAPESP Numbers 2017/01753-3, 2014/19967-1), which enabled this study to be carried out.

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Authors and Affiliations

  1. Centro de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas, Campinas, SP, 13012-970, Brazil

    Caléo Panhoca de Almeida, Natalia Arruda, Jean Fausto de Carvalho Paulino, Gabriel Francesco Janini Bonfante & Luciana Lasry Benchimol-Reis

  2. Escola de Agronomia, Programa de Pós-Graduação em Genética e Melhoramento de Plantas, Universidade Federal de Goiás, Goiânia, GO, Brazil

    Gabriel Mamédio de Freitas

  3. Centro de Educação Superior da Região Sul, Universidade do Estado de Santa Catarina, Laguna, SC, 88790-000, Brazil

    Miklos Maximiliano Bajay

  4. Centro Avançado de Pesquisa em Proteção de Plantas e Saúde Animal, Instituto Biológico, Campinas, SP, 13101-680, Brazil

    Bianca Cristina de Deus & Flávia Rodrigues Alves Patrício

  5. Centro de Grãos e Fibras, Instituto Agronômico de Campinas, Campinas, SP, 13012-970, Brazil

    Sérgio Augusto Morais Carbonell & Alisson Fernando Chiorato

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  1. Caléo Panhoca de Almeida
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Contributions

C.P.A.; N.A.; J.F.C.P.; G.F.J.B.; and B.C.D. conducted the experiments. C.P.A and M.M.B. analyzed the data. C.P.A. wrote the draft. G.M.F. conducted the manuscript edition. F.R.A.P; A.F.C.; S.A.M.; and L.L.B-R supported data curation and conducted review of the manuscript. L.L.B-R idealized the study and was responsible for the project’s funding. All the authors read and approved the final version.

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Correspondence to Caléo Panhoca de Almeida.

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de Almeida, C.P., Arruda, N., de Carvalho Paulino, J.F. et al. Genetic diversity of Pseudocercospora griseola resistance loci in common beans. Trop. plant pathol. 46, 129–138 (2021). https://doi.org/10.1007/s40858-020-00395-0

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