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Two novel recombinant avian leukosis virus isolates from Luxi gamecock chickens

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Abstract

Avian leukosis virus (ALV) is associated with immune suppression, neoplasia, and reduced performance in chickens. In this study, two strains of ALV were isolated from Luxi gamecocks by DF-1 cell culture and identified by PCR, immunofluorescence assay, and sequencing of the viral genome. These strains were found to be novel recombinant viruses with nucleotide sequence identity of over 93.0% in the LTR and 94.4% in U3 to ALV-J, over 95.0% in the 5’UTR to ALV-C, over 93.4% in gp85 to ALV-B, and over 96.0% in gp37 to ALV-E. These results indicate that these two isolates are recombinants between ALV-J, ALV-C, ALV-E and ALV-B.

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Acknowledgements

This work was supported by the Shandong Provincial Natural Science Foundation (ZR201807070202) and the Natural Science Foundation of Shandong Province, China (ZR2019ZD21).

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Authors and Affiliations

  1. Institute of Microbe and Host Health, Linyi University, Linyi, 276005, Shandong, China

    Peikun Wang, Jianrui Niu, Cong Xue, Zhaoqing Han, Amro Abdelazez & Zhang Xinglin

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  1. Peikun Wang
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  2. Jianrui Niu
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  3. Cong Xue
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  4. Zhaoqing Han
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  5. Amro Abdelazez
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  6. Zhang Xinglin
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Contributions

PK and JR designed the study, performed the experiments, and drafted the manuscript. XC carried out virus isolation and identification. XL observed and recorded the results.

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Correspondence to Peikun Wang or Zhang Xinglin.

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The authors declare that they have no conflict of interest.

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Handling Editor: Zhongjie Shi.

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Wang, P., Niu, J., Xue, C. et al. Two novel recombinant avian leukosis virus isolates from Luxi gamecock chickens. Arch Virol 165, 2877–2881 (2020). https://doi.org/10.1007/s00705-020-04799-w

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  • DOI: https://doi.org/10.1007/s00705-020-04799-w

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