Skip to main content
SpringerLink
Log in

Quantification of Plasma and Leukocyte Vitamin C by High Performance Liquid Chromatography with Mass Spectrometric Detection

  • ARTICLES
  • Published:
Journal of Analytical Chemistry Aims and scope Submit manuscript

Abstract

In the present study a high performance liquid chromatographic–mass spectrometric (LC–MS) method was developed for the measurement of ascorbic acid in human plasma and white blood cells. Special attention was paid to the known difficulties of ascorbic acid stability, and a plasma sample preparation method was developed, accordingly. The novel objective of this work was to focus on the determination of plasma ascorbic acid concentrations considering the necessity of accurate determination for further clinical studies related to scurvy induced by various pathological conditions. The presented study describes in detail a validated LC–MS method with adequate accuracy and precision for quantitative determination of ascorbic acid in human plasma. The method was tested on human plasma and leukocyte samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.
Fig. 7.
Fig. 8.

Similar content being viewed by others

REFERENCES

  1. Blokhina, O., Virolainen, E., and Fagerstedt, K.V., Ann. Bot., 2003, vol. 91, p. 179.

    Article  CAS  Google Scholar 

  2. Padayatty, S.J., Katz, A., Wang, Y., Eck, P., Kwon, O., Lee, J.H., Chen, S., Corpe, C., Dutta, S.K., and Levine, M., J. Am. Coll. Nutr., 2003, vol. 22, no. 1, p. 18.

    Article  CAS  Google Scholar 

  3. Libby, A.F. and Stone, I., Orthomol.Psychiatry, 1977, vol. 6, no. 4, p. 300.

    Google Scholar 

  4. Stone, I., NHF/Bull., 1972, vol. 18, no. 10, p. 6.

  5. Levy, T.E., Curing the Incurable, Henderson, NV: Livon Books, 2002.

    Google Scholar 

  6. Will, J.C., Ford, E.S., and Bowman, B.A., Serum Vitamin C Concentrations and Diabetes: Findings from the Third National Health and Nutrition Examination Survey, 1988–1994.

  7. Burk, C.J. and Molodow, R., Am. J. Clin. Dermatol., 2007, vol. 8, no. 2, p. 103.

    Article  Google Scholar 

  8. Pailhous, S., Lamoureux, S., and Caietta, E., Arch. Pediatr., 2015, vol. 22, no. 1, p. 63.

    Article  CAS  Google Scholar 

  9. Lenton, K.J., Sane, A.T., Therriault, H., Cantin, A.M., Payette, H., and Wagner, J.R., Am. J. Clin. Nutr., 2003, vol. 77, no. 1, p. 189.

    Article  CAS  Google Scholar 

  10. Meister, A., Biochem. Pharm., 1992, vol. 44, no. 10, p. 1905.

    Article  CAS  Google Scholar 

  11. Novakova, L., Solich, P., and Solichova, D., TrAC,Trends Anal. Chem., 2008, vol. 27, no. 10, p. 942.

    Article  CAS  Google Scholar 

  12. Bergsten, P., Amitai, G., Kehrl, J., Dhariwal, K.R., Klein, H.G., and Levine, M., J. Biol. Chem., 1990, vol. 265, no. 5, p. 2584.

    CAS  PubMed  Google Scholar 

  13. Bruno, R.S., Leonard, S.W., Atkinson, J., Montine, T.J., Ramakrishnan, R., Bray, T.M., and Traber, M.G., Free Radical Biol. Med., 2006, vol. 40, no. 4, p. 689.

    Article  CAS  Google Scholar 

  14. Ferin, R., Pavao, M.L., and Baptista, J., Clin. Biochem., 2013, vol. 7–8, p. 665.

    Article  Google Scholar 

  15. Viscovich, M., Lykkesfeldt, J., and Poulsen, H.E., Clin. Nutr., 2004, vol. 23, no. 5, p. 1043.

    Article  CAS  Google Scholar 

  16. Shintani, H., Pharm. Anal. Acta, 2013, vol. 4, p. 5.

    Google Scholar 

  17. Robitaille, L. and Hoffer, L.J., Nutr. J., 2015, vol. 15, p. 40.

    Article  Google Scholar 

  18. Lykkesfeldt, J., Cancer Epidemiol., Biomarkers Prev., 2007, vol. 16, no. 11, p. 2513.

    Article  CAS  Google Scholar 

  19. Szultka, M., Buszewska-Forajta, M., Kaliszan, R., and Buszewski, B., Electrophoresis, 2014, vol. 35, no. 4, p. 585.

    Article  CAS  Google Scholar 

  20. Frenich, A.G., Torres, M.E., Vega, A.B., Vidal, J.L., and Bolanos, P.P., J. Agric. Food Chem., 2005, vol. 53, no. 19, p. 7371.

    Article  CAS  Google Scholar 

  21. Spínola, V., Llorent-Martínez, E.J., and Castilho, P.C., J. Chromatogr. A, 2014, vol. 1369, p. 2.

    Article  Google Scholar 

  22. Haswell, L.E., Papadopoulou, E., Newland, N., Shepperd, C.J., and Lowe, F.J., Biomarkers, 2014, vol. 19, no. 5, p. 356.

    Article  CAS  Google Scholar 

  23. Zappielo, C.D., Nicácio, A.E., Manin, L.P., Maldaner, L., and Visentainer, J., J. Braz. Chem. Soc., vol. 30, no. 6, p. 1130.

  24. Chebrolu, K.K., Jayaprakasha, G.K., Yoo, K.S., Jifon, J.L., and Patil, B.S., LWT—Food Sci. Technol., 2012, vol. 47, p. 443.

    Article  CAS  Google Scholar 

  25. Fenoll, J., Martínez, A., Hellín, P., and Flores, P., Food Chem., 2011, vol. 127, p. 340.

    Article  CAS  Google Scholar 

  26. Mazurek, A. and Pankiewicz, U., Acta Sci. Pol., 2012, vol. 11, no. 6, p. 169.

    Google Scholar 

  27. Tarrago-Trani, M.T., Phillips, K.M., and Cotty, M., J. Food. Compos. Anal., 2012, vol. 26, p. 12.

    Article  CAS  Google Scholar 

  28. Hu, L., Li, L., Luo, Z., Yang, J., and Liu, W., J. Chromatogr. Sci., 2012, vol. 50, no. 2, p. 102.

    Article  CAS  Google Scholar 

  29. Kirilov, B., Obreshkova, D., and Tsvetkova, D., Int. J. Pharm. Pharm. Sci., 2012, vol. 4, no. 1, p. 300.

    CAS  Google Scholar 

  30. Guideline on Bioanalytical Method Validation, EMEA/CHMP/EWP/192217/2009, European Medicines Agency, 21 July 2011.

Download references

Funding

The work was supported by Internal Research Grants of the University of Medicine and Pharmacy of Tîrgu Mureş, România (Contract no. 17800/12/22.12.2015).

Author information

Authors and Affiliations

  1. Department of Public Health Medicine, University of Pécs, HU-7624, Pécs, Hungary

    Attila Szőcs & István Kiss

  2. Faculty of Pharmacy, University of Medicine, Pharmacy, Sciences and Technology of Targu Mures, RO-540139, Târgu Mureş, Romania

    Szende Vancea & Gabriella Donáth-Nagy

Authors
  1. Attila Szőcs
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Szende Vancea
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. István Kiss
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Gabriella Donáth-Nagy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Szende Vancea.

Ethics declarations

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Attila Szőcs, Vancea, S., Kiss, I. et al. Quantification of Plasma and Leukocyte Vitamin C by High Performance Liquid Chromatography with Mass Spectrometric Detection. J Anal Chem 75, 1168–1176 (2020). https://doi.org/10.1134/S1061934820090038

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1061934820090038

Keywords:

Search

Navigation