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Detection of macromolecular inversion–induced structural changes in osteosarcoma cells by FTIR microspectroscopy

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Abstract

Fourier transform infrared (FTIR) microspectroscopy provides a biochemical fingerprint of the cells. In this study, chemical changes in 143B osteosarcoma cells were investigated using FTIR analysis of cancer cells after their treatment with polymeric invertible micellar assemblies (IMAs) and curcumin-loaded IMAs and compared with untreated osteosarcoma cells. A comprehensive principal component analysis (PCA) was applied to analyze the FTIR results and confirm noticeable changes in cell surface chemical structures in the fingerprint regions of 1480–900 cm−1. The performed clustering shows visible differences for all investigated groups of cancer cells. It is demonstrated that a combination of FTIR microspectroscopy with PCA can be an efficient approach in determining interactions of osteosarcoma cells and drug-loaded polymer micellar assemblies.

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Funding

This study was funded by Mayo Clinic.

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Authors and Affiliations

  1. Department of Orthopedics, Mayo Clinic, Rochester, MN, 55905, USA

    Vitalii Serdiuk, Kristen L. Shogren, Michael Yaszemski & Avudaiappan Maran

  2. Department of Coatings & Polymeric Materials, North Dakota State University, Fargo, ND, 58105, USA

    Vitalii Serdiuk, Bakhtiyor Rasulev & Andriy Voronov

  3. Department of Organic Chemistry, Lviv Polytechnic National University, Lviv, 79013, Ukraine

    Vitalii Serdiuk & Tetiana Kovalenko

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  1. Vitalii Serdiuk
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  2. Kristen L. Shogren
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Correspondence to Andriy Voronov.

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Serdiuk, V., Shogren, K.L., Kovalenko, T. et al. Detection of macromolecular inversion–induced structural changes in osteosarcoma cells by FTIR microspectroscopy. Anal Bioanal Chem 412, 7253–7262 (2020). https://doi.org/10.1007/s00216-020-02858-4

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  • DOI: https://doi.org/10.1007/s00216-020-02858-4

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