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Green Synthesis, Characterization, and Antibacterial Investigation of Synthesized Gold Nanoparticles (AuNPs) from Garcinia kola Pulp Extract

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Abstract

The properties of gold nanoparticles, such as conductivity, half-life, catalytic nature, improved solubility efficiency, chemical stability, and its mechanism in binding with bacterial cell wall to induce cell death, have increased its medicinal applications. This study investigated the antibacterial property of gold nanoparticles (AuNPs) synthesized from Garcinia kola pulp extract. The ripe fruits of Garcinia kola were obtained, and the pulps were removed, air dried, pulverized, extracted, and mixed with gold chloride solution following standard procedure. The synthesized AuNPs were characterized using ultraviolet visible spectrophotometer (UV-visible), Fourier transform infrared (FTIR) spectrophotometer, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive X-ray (EDX). The antibacterial examination was carried out on four clinical isolate bacteria using agar diffusion technique. The UV-visible analysis confirmed the formation of stable AuNPs at pH 7 in the wavelength region of 564 nm. The FTIR analysis confirmed that the secondary metabolites with –OH functional groups serve as the reducing agent for the synthesis of AuNPs. The morphological assessment of the synthesized AuNPs revealed spherical-shaped particles. The zones of inhibition of the synthesized AuNPs against test bacteria ranges from 3 to 13 mm while that of the control was in the range of 14 to 22 mm. This study provided an environmentally friendly approach in the synthesis of AuNPs from Garcinia kola pulps and also ascertained the medicinal application of the synthesized AuNPs as an antibacterial agent.

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References

  1. Balouiri M, Sadiki SK, Ibnsouda M (2016) Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 6(2):71–79

    Article  Google Scholar 

  2. Saleem M, Nazir M, Ali MS, Hussain H, Lee YS, Riaz N, Jabbar A (2010) Antimicrobial natural products: an update on future antibiotic drug candidates. Nat Prod Rep 27:238–254

    Article  CAS  Google Scholar 

  3. Freire-Moran L, Aronsson B, Manz C (2011) Critical shortage of new antibiotics in development against multidrug-resistant bacteria - time to react is now. Drug Res Updates 14(2):118–124

    Article  Google Scholar 

  4. Chaudhary A, Singh A (2016) Macrocyclic complexes: a new way forward into the medicinal world. Int J Adv Res 4(9):1004–1015

    Article  CAS  Google Scholar 

  5. Akintelu SA, Abiola BE, Ajayi SO, Olabemiwo OM (2018) Quantification and preliminary estimation of toxic effects of polycyclic aromatic hydrocarbon in some antimalarial herbal drugs in Southwest Nigeria. Bull Pharm Res 8(1):152

    Google Scholar 

  6. Akintelu SA, Abiola BE, Ajayi SO, Olabemiwo OM (2019) Preliminary study of the effects of some herbal drugs on the heamatological and biochemical parameter of Winster albino rats. Int J Therm Sci 8(10):26–31

    Google Scholar 

  7. Yu X, Jaio Y, Chan Q (2016) Applications of gold nanoparticles in biosensors. Nano Life 6(2):1642001

    Article  CAS  Google Scholar 

  8. Akintelu SA, Folorunso AS, Folorunso FA, Oyebamiji AK (2020) Green synthesis of copper oxide nanoparticles for biomedical application and environmental remediation. Heliyon. 6:e04508. https://doi.org/10.1016/j.heliyon.2020.e04508

    Article  PubMed  PubMed Central  Google Scholar 

  9. Akintelu SA, Folorunso AS (2020) A review on green synthesis of zinc oxide nanoparticles using plant extracts and its biomedical applications. BioNanoSci. https://doi.org/10.1007/s12668-020-00774-6

  10. Husseiny MI, Abd El-Aziz M, Badr Y, Mahmoud MA (2006) Biosynthesis of gold nanoparticles using Pseudomonas aeruginosa. Spect Acta Part A 67:1003–1006

    Article  Google Scholar 

  11. Akintelu SA, Folorunso AS (2019) Biosynthesis, characterization and antifungal investigation of Ag-Cu nanoparticles from bark extracts of Garcina kola. Stem Cells 10(4):30–37

    Google Scholar 

  12. Akintelu SA, Folorunso AS, Oyebamiji AK, Erazua EA (2019) Antibacterial potency of silver nanoparticles synthesized using Boerhaavia diffusa leaf extract as reductive and stabilizing agent. Int J Pharm Sci Res 10(12):374–380

    CAS  Google Scholar 

  13. Mandal D, Bolander ME, Mukhopadhyay D, Sarkar G, Mukherjee P (2006) The use of microorganisms for the formation of metal nanoparticles and their application. Appl Microbiol. Biotechnol. 69:485–492

    CAS  Google Scholar 

  14. Akintelu SA, Folorunso AS (2019) Characterization and antimicrobial investigation of synthesized silver nanoparticles from annona muricata leaf extracts. J Nanotechnol Nanomed Nanobiotechnol 6:1–6

    Google Scholar 

  15. Usunomena U (2012) A review of some African medicinal plants. Int J Pharm Bio Sci 3:1–11

    Google Scholar 

  16. Onasanwo SA, Rotu RA (2016) Antinociceptive and anti-inflammatory potentials of kolaviron: mechanisms of action. J Basic Clin Physiol Pharmacol 27:363–370

    CAS  PubMed  Google Scholar 

  17. Folorunso AS, Oyebamiji AK, Erazua EA, Akintelu SA (2019) The exploration of antifungal activity of garcinia kola seed as novel antifungal agent. Int J Trad Nat Med 9(1):41–49

    CAS  Google Scholar 

  18. Sacandé M, Pritchard HW (2004) Seed research network on African trees for conservation and sustainable use. For Genet Resour 31:31–35

    Google Scholar 

  19. Tchoundjeu Z, Asaah EK, Anegbeh P, Degrande A, Mbile P, Facheux C, Tsobeng A, Atangana AR, Ngo-Mpeck ML, Simons AJ (2006) Putting participatory domestication into practice in West and Central Africa. For Trees Livelihoods 16:53–69

    Article  Google Scholar 

  20. Franzel S, Kindt R (2012) Species priority setting procedures. In Dawson, I, Harwood, C, Jamnadass, R, Beniest, J Eds. Agroforestry tree domestication: a primer; World Agroforestry Centre: Nairobi, Kenya. 36–45

  21. Stevens PF Angiosperm phylogeny website. Available online: http://www.mobot.org/MOBOT/research/APweb/ (accessed on 8 August 2019)

  22. Nwaehujor CO, Udegbunam RI, Ode JO, Udegbunam SO (2015) Analgesic anti-inflammatory anti-pyretic activities of Garcinia hydroxybiflavanonol (GB1) from Garcinia kola. J Korean Soc Appl Biol Chem 58:91–96

    Article  CAS  Google Scholar 

  23. Tshibangu PT, Kapepula PM, Kapinga MJK, Lupona HK, Ngombe NK, Kalenda DT, Jansen O, Wauters JN, Tits M, Angenot L (2016) Fingerprinting and validation of a LC-DAD method for the analysis of biflavanones in Garcinia kola -based antimalarial improved traditional medicines. J Pharm Biomed Anal 12(8):382–390

    Article  Google Scholar 

  24. Anna M, Olga L, Zacharie T, Patrick V, Vladimír V, Ondˇrej P, Bohdan L (2019) Medicinal potential, utilization and domestication status of bitter kola (Garcinia kola Heckel) in West and Central Africa. Forests 10(124):1–28

    Google Scholar 

  25. Folorunso A, Akintelu S, Oyebamiji AK, Ajayi S, Abiola B, Abdusalam I, Morakinyo A (2019) Biosynthesis, characterization and antimicrobial activity of gold nanoparticles from leaf extracts of Annona muricata. J Nanostruct Chem 9:111–117

    Article  CAS  Google Scholar 

  26. Kasthuri J, Veerapandian S, Rajendiran N (2009) Biological synthesis of silver and gold nanoparticles using apiin as reducing agent. Colloids Surf B: Biointerfaces 68:55–60

    Article  CAS  Google Scholar 

  27. Khan S, Bakht J, Syed F (2018) Green synthesis of gold nanoparticles using acer pentapomicum leaves extract its characterization, antibacterial, antifungal and antioxidant bioassay. Dig J Nanomat Biostruct 2(13):579–589

    Google Scholar 

  28. Masumeh N (2015) Biosynthesis of gold nanoparticles using plant extracts. Bioprocess Biosyst Eng 38:1–14

    Article  Google Scholar 

  29. Singh AK, Srivastava ON (2015) One-step green synthesis of gold nanoparticles using black cardamom and effect of pH on its synthesis. Nanoscale Res Lett 10:353

    Article  Google Scholar 

  30. Umamaheswari C, Lakshmanan A, Nagarajan NS (2018) Green synthesis, characterization and catalytic degradation studies of gold nanoparticles against Congo red and methyl orange. J Photochem Photobiol B Biol 178:33–39

    Article  CAS  Google Scholar 

  31. Muniyappan N, Nagarajan NS (2014) Green synthesis of silver nanoparticles with Dalbergia spinosa leaves and their applications in biological and catalytic activities. Process Biochem 49:1054–1061

    Article  CAS  Google Scholar 

  32. Xiao Y, Fan J, Chen Y, Rui X, Zhang Q, Dong M (2016) Enhanced total phenolic and isoflavone aglycone content, antioxidant activity and DNA damage protection of soybeans processed by solid state fermentation with Rhizopus oligosporus RT-3. RSC Adv 6:29741–29756

    Article  CAS  Google Scholar 

  33. Babu PJ, Sharma P, Saranya S, Bora U (2013) Synthesis of gold nanoparticles using ethonolic leaf extract of Bacopa monnieri and UV irradiation. Mater Lett 93:431–434

    Article  CAS  Google Scholar 

  34. Bakur A, Niu Y, Kuang H, Chen Q (2019) Synthesis of gold nanoparticles derived from mannosylerythritol lipid and evaluation of their bioactivities. AMB Express 9(62):2–9

    Google Scholar 

  35. Akintelu SA, Folorunso AS, Ademosun OT (2019) Instrumental characterization and antibacterial investigation of silver nanoparticles synthesized From Garcinia kola leaf. J Drug Del Ther 9(6-s):58–64

    Article  CAS  Google Scholar 

  36. Lei W, Jianwei X, Ye Y, Han L, Feng L (2019) Synthesis of gold nanoparticles from leaf Panax notoginseng and its anticancer activity in pancreatic cancer PANC-1 cell lines. Artificial Cells, Nanomed Biotechnol 1(47):1216–1223

    Google Scholar 

  37. Mendoza RM, Baybay ZK, Fernando LM, Montecillo AD, Ilag LL, Villegas LC (2019) Characterization of gold nanoparticles produced by biogenic synthesis using Serratia marcescens NBL1001. IOP Conf Series: Earth Environ Sci 230:12103

    Article  Google Scholar 

  38. Ratul KD, Devangana B (2019) Microwave-mediated green synthesis of gold and silver nanoparticles from fruit peel aqueous extract of Solanum melongena L. and study of antimicrobial property of silver nanoparticles. Nanotechnol Environ Eng 4(5):1–6

    Google Scholar 

  39. Nikparast Y, Saliani M (2018) Synergistic effect between phyto-synthesized silver nanoparticles and ciprofloxacin antibiotic on some pathogenic bacterial strains. J Med Bacteriol 7:36–43

    CAS  Google Scholar 

  40. Venilla S, Suresh S, Lakshmipathy M, Mohd RJ, Jiban P (2019) Eco-friendly approach in synthesis of silver nanoparticles and evaluation of optical, surface morphological and antimicrobial properties. J Nanostruct Chem 9:153–162

    Article  Google Scholar 

  41. Oyebamiji AK, Akintelu SA, Folorunso AS, Abiola BE, Ajayi SO, Abdusalam IO, Morakinyo AE (2019) Computational and experimental studies on antimicrobial activity of the bark of annona muricata against some selected human pathogenic bacteria and fungi. Int J Mod Chem 11(1):9–27

    Article  Google Scholar 

  42. Ravichandran K, Snega S, Jabena BN, Swaminathan K, Sakthivel B, Rene CL (2014) Enhancement in the antibacterial efficiency of ZnO nanopowders by tuning the shape of the nanograins through fluorine doping. Superlattice Microst 69:17–28

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. MOE Key Laboratory of Cluster Science, Beijing Key Laboratory of Photoelectronic Electrophotonic Conversion Materials, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, 102488, People’s Republic of China

    Sunday Adewale Akintelu & Bo Yao

  2. Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria

    Sunday Adewale Akintelu

  3. Department of Chemistry, Louisiana State University, Louisiana, USA

    Aderonke Similoluwa Folorunso

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  1. Sunday Adewale Akintelu
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  2. Bo Yao
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  3. Aderonke Similoluwa Folorunso
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Correspondence to Aderonke Similoluwa Folorunso.

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Akintelu, S.A., Yao, B. & Folorunso, A.S. Green Synthesis, Characterization, and Antibacterial Investigation of Synthesized Gold Nanoparticles (AuNPs) from Garcinia kola Pulp Extract. Plasmonics 16, 157–165 (2021). https://doi.org/10.1007/s11468-020-01274-9

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