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Changes in the mycorrhizal fungal community in host roots over five host generations under low and high phosphorus conditions

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Abstract

Aims

We tested whether arbuscular mycorrhizal fungal (AMF) community might shift as host plant generations advance under a low phosphorus (P) soil.

Methods

Two greenhouse experiments were conducted using Medicago truncatula as the host plant, six AMF species, and two soil P levels. Experiment 1 assessed changes in AMF composition in roots and soil during five generations of host plant grown in low-P (5.7 mg kg−1) and high-P soil (24.0 mg kg−1). Experiment 2 tested the effects of single AMF species on host plant growth in low-P and high-P soil.

Results

Experiment 1 showed that AMF species composition at the OTU level in roots and soil significantly changed as host generations advanced, and that the pattern of change differed in low-P vs. high-P soil. In low-P soil, the dominant OTU in both roots and soil shifted from OTU-50 (Glomus tortuosum) to OTU-49 (Funneliformis geosporum) as host generations advanced. In high-P soil, the AMF community in roots and soil was dominated by OTU-50 in first two generations but OTU-49 and OTU-50 were co-dominant in the last three generations. Experiment 2 showed that mycorrhizal dependency indices based on plant biomass and P content in plant biomass, and spore numbers were higher in host plants with F. geosporum than other AMF fungal treatments in low-P soil.

Conclusion

As the host M. truncatula grew in a low-P soil over five generations, AMF community in root and soil shifted to a composition with F. geosporum as dominant species who was effective in foraging for P.

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Funding

This research was supported by the National Key Research and Development Program of China (No. 2016YFC0502703–4) and the National Natural Science Foundation of China (Nos. 31,470,483, and 31,570,411).

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou, 310058, China

    Shijun Liu, Jing Xu, Hong Huang, Jiani Zhu, Jianjun Tang & Xin Chen

  2. The Institute of Environmental Resources and Soil Fertilizers, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Jing Xu

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  1. Shijun Liu
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  2. Jing Xu
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  3. Hong Huang
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  4. Jiani Zhu
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Correspondence to Jianjun Tang or Xin Chen.

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Liu, S., Xu, J., Huang, H. et al. Changes in the mycorrhizal fungal community in host roots over five host generations under low and high phosphorus conditions. Plant Soil 456, 27–41 (2020). https://doi.org/10.1007/s11104-020-04694-y

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