Abstract
Nicotiana alata is a model species used to study gametophytic self-incompatibility (SI), controlled by the S-locus (encoding S-RNase and SLF proteins expressed in pistil and pollen, respectively) as well as by modifier genes (such as 120 kDa, HT-b and Trxh). A previous study revealed that SI in N. alata accession S2S2 was plastic, and that SI was leaky with senescence of the pistil and plant. Here, we further explored its regulatory mechanisms. Reverse transcription quantitative PCR (RT-qPCR) and western blot results showed that during the senescence of pistil in S2S2, 120 kDa and HT-b were transcribed and translated at lower levels, while marked residual S-RNase activity was detected. The transcripts of Trxh varied irregularly. Only the transcription and translation of 120 kDa declined with the plant senescence. According to the results, PSF in S2S2 may be associated with down-regulated expression of 120 kDa and HT-b, while S-RNase and Trxh seem to be unrelated to this phenotype. These findings deepen our understanding of the regulatory mechanisms of SI plasticity in N. alata.
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Acknowledgements
We are very grateful to Professor Xuejun Chen (Yunnan Academy of Tobacco Agricultural Sciences) for providing the seeds and genotype of the N.alata material. This study was supported by Grants 31560419 and 31660067 from the National Natural Science Fund in China, Grants 2017KY05 and 2018530000241034 from Yuxi Zhongyan Tobacco Seed Co., Ltd..
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Liao, J., Wei, X., Chen, Z. et al. Research on the regulatory mechanisms of self-incompatibility plasticity in Nicotiana alata. Euphytica 216, 149 (2020). https://doi.org/10.1007/s10681-020-02687-7
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DOI: https://doi.org/10.1007/s10681-020-02687-7