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Methemoglobin and the Activities of Catalase and Superoxide Dismutase in Nucleated Erythrocytes of Scorpaena porcus (Linnaeus, 1758) under Experimental Hypoxia (in vitro)

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Abstract

The effect of hypoxia on nucleated red blood cells of the black scorpionfish (Scorpaena porcus) was studied in vitro. Deep hypoxia (the oxygen concentration was less than 1 mg O2 L–1; the norm was 7–8 mg O2 L–1) led to the transition of a part of the hemoglobin molecules to the ferric state (methemoglobin). The maximum increase in the concentration of methemoglobin was 32%. The accumulation of methemoglobin in red blood cells was accompanied by an increase in the activity of catalase and superoxide dismutase and a decrease in the content of reactive oxygen species in the cytoplasm of cells. It was shown that the formation of methemoglobin did not cause damage to the cytoplasmic membranes of red blood cells. The percentage of red blood cell lysis in deoxygenated (less than 1.0 mg O2 L–1) suspensions quantitatively coincided with the control values.

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Funding

This work was performed within the framework of the State Task, State Registration no. AAAA-A18-118021490093-4; and with partial financial support of the Russian Foundation for Basic Research, project no. 16-04-00135.

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Authors and Affiliations

  1. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, 299011, Sevastopol, Russia

    A. A. Soldatov, A. Y. Andreeva, T. A. Kukhareva & T. I. Andreyenko

  2. Sevastopol State University, 299053, Sevastopol, Russia

    A. A. Soldatov & T. I. Andreyenko

  3. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 194223, St. Petersburg, Russia

    A. Y. Andreeva

Authors
  1. A. A. Soldatov
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  2. A. Y. Andreeva
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  3. T. A. Kukhareva
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  4. T. I. Andreyenko
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Correspondence to A. A. Soldatov.

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The authors declare that there is no conflict of interest.

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All applicable international, national and institutional principles for the care and use of animals in the performance of work have been observed.

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Translated by E. Puchkov

Abbreviations: Hb, hemoglobin; HbO2, oxyhemoglobin; MtHb, methemoglobin; NADH, nicotinamide adenine dinucleotide (reduced); CAT, catalase; SOD, superoxide dismutase; ROS, reactive oxygen species.

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Soldatov, A.A., Andreeva, A.Y., Kukhareva, T.A. et al. Methemoglobin and the Activities of Catalase and Superoxide Dismutase in Nucleated Erythrocytes of Scorpaena porcus (Linnaeus, 1758) under Experimental Hypoxia (in vitro). BIOPHYSICS 65, 452–459 (2020). https://doi.org/10.1134/S0006350920030197

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