Abstract
Epidermal leaf waxes of terrestrial higher plants have been widely utilized for the reconstructions of paleoenvironment and paleoclimate in peat deposits. In this study, specimens of four plant species growing in both peatland and non-peatland habitats were retrieved to compare their molecular, carbon (δ13C) and hydrogen (δ2H) isotopic compositions of leaf wax n-alkanes from a closed subalpine basin in Central China. Three of the four species show quite higher total concentrations of n-alkanes in the relatively dry non-peatland setting than in the peatland. In addition, the δ2H values of long-chain n-alkanes are generally less depleted in the peatland and are comparable among different plant species, which is interpreted as the influence of inundation condition and the possible limited supply of photosynthetic products. This study reveals different patterns of plant wax molecular and isotopic compositions between peatland and the surrounding non-peatland conditions, and confirms the paleoenvironmental potential of leaf wax ratios on the peat sequences.
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References Cited
Bush, R. T., McInerney, F. A., 2013. Leaf Wax n-Alkane Distributions in and across Modern Plants: Implications for Paleoecology and Chemotaxonomy. Geochimica et Cosmochimica Acta, 117: 161–179. https://doi.org/10.1016/j.gca.2013.04.016
Carr, A. S., Boom, A., Grimes, H. L., et al., 2014. Leaf Wax n-Alkane Distributions in Arid Zone South African Flora: Environmental Controls, Chemotaxonomy and Palaeoecological Implications. Organic Geochemistry, 67: 72–84. https://doi.org/10.1016/j.orggeochem.2013.12.004
Chambers, F. M., Booth, R. K., de Vleeschouwer, F., et al., 2012. Development and Refinement of Proxy-Climate Indicators from Peats. Quaternary International, 268: 21–33. https://doi.org/10.1016/j.quaint.2011.04.039
Cormier, M. A., Werner, R. A., Leuenberger, M. C., et al., 2019. 2H-Enrichment of Cellulose and n-Alkanes in Heterotrophic Plants. Oecologia, 189(2): 365–373. https://doi.org/10.1007/s00442-019-04338-8
Cormier, M. A., Werner, R. A., Sauer, P. E., et al., 2018. 2H-Fractionations during the Biosynthesis of Carbohydrates and Lipids Imprint a Metabolic Signal on the δ2H Values of Plant Organic Compounds. New Phytologist, 218(2): 479–491. https://doi.org/10.1111/nph.15016
Diefendorf, A. F., Freimuth, E. J., 2017. Extracting the Most from Terrestrial Plant-Derived n-Alkyl Lipids and Their Carbon Isotopes from the Sedimentary Record: A Review. Organic Geochemistry, 103: 1–21. https://doi.org/10.1016/j.orggeochem.2016.10.016
Eglinton, G., Hamilton, R. J., 1967. Leaf Epicuticular Waxes. Science, 156(3780): 1322–1335. https://doi.org/10.1126/science.156.3780.1322
Farrimond, P., Flanagan, R. L., 1996. Lipid Stratigraphy of a Flandrian Peat Bed (Northumberland, UK): Comparison with the Pollen Record. The Holocene, 6(1): 69–74. https://doi.org/10.1177/095968369600600108
Freimuth, E. J., Diefendorf, A. F., Lowell, T. V., 2017. Hydrogen Isotopes of n-Alkanes and n-Alkanoic Acids as Tracers of Precipitation in a Temperate Forest and Implications for Paleorecords. Geochimica et Cosmochimica Acta, 206: 166–183. https://doi.org/10.1016/j.gca.2017.02.027
Huang, X. Y., Meyers, P. A., 2019. Assessing Paleohydrologic Controls on the Hydrogen Isotope Compositions of Leaf Wax n-Alkanes in Chinese Peat Deposits. Palaeogeography, Palaeoclimatology, Palaeoecology, 516: 354–363. https://doi.org/10.1016/j.palaeo.2018.12.017
Huang, X. Y, Zhao, B. Y, Wang, K., et al., 2018a. Seasonal Variations of Leaf Wax n-Alkane Molecular Composition and δD Values in Two Subtropical Deciduous Tree Species: Results from a Three-Year Monitoring Program in Central China. Organic Geochemistry, 118: 15–26. https://doi.org/10.1016/j.orggeochem.2018.01.009
Huang, X. Y., Pancost, R. D., Xue, J. T., et al., 2018b. Response of Carbon Cycle to Drier Conditions in the Mid-Holocene in Central China. Nature Communications, 9(1): 1369. https://doi.org/10.1038/s41467-018-03804-w
Huang, X., Zhang, Z., Wang, H., et al., 2017. Overview on Critical Zone Observatory at Dajiuhu Peatland, Shennongjia. Earth Science, 42: 1026–1038 (in Chinese with English Abstract)
Huang, X., Xie, S., 2016. An Overview of the Molecular Paleoclimate in Peat Deposits. Quaternary Sciences, 36(3): 666–675 (in Chinese with English Abstract)
Huang, X. Y., Xue, J. T., Meyers, P. A., et al., 2014. Hydrologic Influence on the δ13C Variation in Long Chain n-Alkanes in the Dajiuhu Peatland, Central China. Organic Geochemistry, 69: 114–119. https://doi.org/10.1016/j.orggeochem.2014.01.016
Inglis, G. N., Naafs, B. D. A., Zheng, Y. H., et al., 2019. δ13C Values of Bacterial Hopanoids and Leaf Waxes as Tracers for Methanotrophy in Peatlands. Geochimica et Cosmochimica Acta, 260: 244–256. https://doi.org/10.1016/j.gca.2019.06.030
Kahmen, A., Schefuß, E., Sachse, D., 2013a. Leaf Water Deuterium Enrichment Shapes Leaf Wax n-Alkane δD Values of Angiosperm Plants I: Experimental Evidence and Mechanistic Insights. Geochimica et Cosmochimica Acta, 111: 39–49. https://doi.org/10.1016/j.gca.2012.09.003
Kahmen, A., Hoffmann, B., Schefuß, E., et al., 2013b. Leaf Water Deuterium Enrichment Shapes Leaf Wax n-Alkane 6D Values of Angiosperm Plants II: Observational Evidence and Global Implications. Geochimica et Cosmochimica Acta, 111: 50–63. https://doi.org/10.1016/j.gca.2012.09.004
Li, J., Li, J., Dang, H., et al., 2007. Vegetation and Conservation of Strategy of Dajiuhu Wetland Park in Shennongjiaregion. Journal of Wuhan Botanical Research, 25(6): 605–611 (in Chinese with English Abstract)
Liu, J. Z., An, Z. S., 2019. Variations in Hydrogen Isotopic Fractionation in Higher Plants and Sediments across Different Latitudes: Implications for Paleohydrological Reconstruction. Science of the Total Environment, 650: 470–478. https://doi.org/10.1016/j.scitotenv.2018.09.047
Luo, T., Lun, Z., Gu, Y., et al., 2015. Plant Community Survey and Ecological Protection of Dajiuhu Wetlands in Shennongjia Area. Wetland Science, 13: 153–160 (in Chinese with English Abstract)
Naafs, B. D. A., Inglis, G. N., Blewett, J., et al., 2019. The Potential of Biomarker Proxies to Trace Climate, Vegetation, and Biogeochemical Processes in Peat: A Review. Global and Planetary Change, 179: 57–79. https://doi.org/10.1016/j.gloplacha.2019.05.006
Newberry, S. L., Kahmen, A., Dennis, P., et al., 2015. n-Alkane Biosynthetic Hydrogen Isotope Fractionation is not Constant Throughout the Growing Season in the Riparian Tree Salix Viminalis. Geochimica et Cosmochimica Acta, 165: 75–85. https://doi.org/10.1016/j.gca.2015.05.001
Nott, C. J., Xie, S. C., Avsejs, L. A., et al., 2000. n-Alkane Distributions in Ombrotrophic Mires as Indicators of Vegetation Change Related to Climatic Variation. Organic Geochemistry, 31(2/3): 231–235. https://doi.org/10.1016/s0146-6380(99)00153-9
Nichols, J. E., Peteet, D. M., Moy, C. M., et al., 2014. Impacts of Climate and Vegetation Change on Carbon Accumulation in a South-Central Alaskan Peatland Assessed with Novel Organic Geochemical Techniques. The Holocene, 24(9): 1146–1155. https://doi.org/10.1177/0959683614540729
Nichols, J., Booth, R. K., Jackson, S. T., et al., 2010. Differential Hydrogen Isotopic Ratios of Sphagnum and Vascular Plant Biomarkers in Ombrotrophic Peatlands as a Quantitative Proxy for Precipitation—Evaporation Balance. Geochimica et Cosmochimica Acta, 74(4): 1407–1416. https://doi.org/10.1016/j.gca.2009.11.012
Nichols, J. E., Walcott, M., Bradley, R., et al., 2009. Quantitative Assessment of Precipitation Seasonality and Summer Surface Wetness Using Ombrotrophic Sediments from an Arctic Norwegian Peatland. Quaternary Research, 72(3): 443–451. https://doi.org/10.1016/j.yqres.2009.07.007
Nichols, J. E., Booth, R. K., Jackson, S. T., et al., 2006. Paleohydrologic Reconstruction Based on n-Alkane Distributions in Ombrotrophic Peat. Organic Geochemistry, 37(11): 1505–1513. https://doi.org/10.1016/j.orggeochem.2006.06.020
Pancost, R. D., Baas, M., van Geel, B., et al., 2002. Biomarkers as Proxies for Plant Inputs to Peats: An Example from a Sub-Boreal Ombrotrophic Bog. Organic Geochemistry, 33(7): 675–690. https://doi.org/10.1016/s0146-6380(02)00048-7
Sachse, D., Billault, I., Bowen, G. J., et al., 2012. Molecular Paleohydrology: Interpreting the Hydrogen-Isotopic Composition of Lipid Biomarkers from Photosynthesizing Organisms. Annual Review of Earth and Planetary Sciences, 40(1): 221–249. https://doi.org/10.1146/annurev-earth-042711-105535
Schmidt, H. L., Werner, R. A., Eisenreich, W., 2003. Systematics of 2H Patterns in Natural Compounds and Its Importance for the Elucidation of Biosynthetic Pathways. Phytochemistry Reviews, 2(1/2): 61–85. https://doi.org/10.1023/b:phyt.0000004185.92648.ae
Seki, O., Meyers, P. A., Yamamoto, S., et al., 2011. Plant-Wax Hydrogen Isotopic Evidence for Postglacial Variations in Delivery of Precipitation in the Monsoon Domain of China. Geology, 39(9): 875–878. https://doi.org/10.1130/g32117.1
Sessions, A. L., 2016. Factors Controlling the Deuterium Contents of Sedimentary Hydrocarbons. Organic Geochemistry, 96: 43–64. https://doi.org/10.1016/j.orggeochem.2016.02.012
Smith, F., Wing, S., Freeman, K., 2007. Magnitude of the Carbon Isotope Excursion at the Paleocene-Eocene Thermal Maximum: The Role of Plant Community Change. Earth and Planetary Science Letters, 262(1/2): 50–65. https://doi.org/10.1016/j.epsl.2007.07.021
Wang, R. C., Wang, H. M., Xiang, X., et al., 2018. Temporal and Spatial Variations of Microbial Carbon Utilization in Water Bodies from the Dajiuhu Peatland, Central China. Journal of Earth Science, 29(4): 969–976. https://doi.org/10.1007/s12583-017-0818-5
Wang, X. X., Huang, X. Y., Sachse, D., et al., 2016. Molecular Paleoclimate Reconstructions over the last 9 Ka from a Peat Sequence in South China. Plos One, 11(8): e0160934. https://doi.org/10.1371/journal.pone.0160934
Xu, Y., Wang, H. M., Xiang, X., et al., 2019. Vertical Variation of Nitrogen Fixers and Ammonia Oxidizers along a Sediment Profile in the Dajiuhu Peatland, Central China. Journal of Earth Science, 30(2): 397–406. https://doi.org/10.1007/s12583-018-0982-2
Yamamoto, S., Kawamura, K., Seki, O., et al., 2010a. Environmental Influences over the Last 16 ka on Compound-Specific δ13C Variations of Leaf Wax n-Alkanes in the Hani Peat Deposit from Northeast China. Chemical Geology, 277(3/4): 261–268. https://doi.org/10.1016/j.chemgeo.2010.08.009
Yamamoto, S., Kawamura, K., Seki, O., et al., 2010b. Paleoenvironmental Significance of Compound-Specific δ13C Variations in n-Alkanes in the Hongyuan Peat Sequence from Southwest China over the Last 13 ka. Organic Geochemistry, 41(5): 491–497. https://doi.org/10.1016/j.orggeochem.2010.01.006
Zhao, B. Y., Zhang, Y. M., Huang, X. Y., et al., 2018. Comparison of n-Alkane Molecular, Carbon and Hydrogen Isotope Compositions of Different Types of Plants in the Dajiuhu Peatland, Central China. Organic Geochemistry, 124: 1–11. https://doi.org/10.1016/j.orggeochem.2018.07.008
Zhao, K., Sun, G., Yang, Y., et al., 1999. Mires in China. Science Press, Beijing. 559–562 (in Chinese with English Abstract)
Zheng, Y. H., Xie, S. C., Liu, X. M., et al., 2009. n-Alkanol Ratios as Proxies of Paleovegetation and Paleoclimate in a Peat-Lacustrine Core in Southern China since the Last Deglaciation. Frontiers of Earth Science in China, 3(4): 445–451. https://doi.org/10.1007/s11707-009-0052-2
Zheng, Y. H., Singarayer, J. S., Cheng, P., et al., 2014. Holocene Variations in Peatland Methane Cycling Associated with the Asian Summer Monsoon System. Nature Communications, 5: 5631. https://doi.org/10.1038/ncomms5631
Zhou, W. J., Xie, S. C., Meyers, P. A., et al., 2005. Reconstruction of Late Glacial and Holocene Climate Evolution in Southern China from Geolipids and Pollen in the Dingnan Peat Sequence. Organic Geochemistry, 36(9): 1272–1284. https://doi.org/10.1016/j.orggeochem.2005.04.005
Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 41877317), the State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan (No. GBL11612), and the fundamental research funds for the central universities (Nos. CUGCJ1703, CUGQY1902). Binyan Zhao, Meiling Zhao, Xiaofang Yu, and Kui Wang are thanked for their help in the field and during lipid extraction and analysis. The final publication is available at Springer via https://doi.org/10.1007/s12583-020-1322-x.
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Yan, C., Zhang, Y., Zhang, Y. et al. Habitat Influence on the Molecular, Carbon and Hydrogen Isotope Compositions of Leaf Wax n-Alkanes in a Subalpine Basin, Central China. J. Earth Sci. 31, 845–852 (2020). https://doi.org/10.1007/s12583-020-1322-x
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DOI: https://doi.org/10.1007/s12583-020-1322-x