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An On-chip Chemiluminescent Immunoassay for Bacterial Detection using in Situ-synthesized Cadmium Sulfide Nanowires with Passivation Layers

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Abstract

The passivation layers of an in situ-synthesized cadmium sulfide (CdS) nanowire (NW) photosensor were prepared for two reasons: (1) to improve the physical stability of the NW on an interdigitated electrode and (2) to enhance the immobilization efficiency of proteins for the on-chip immunoassay. The passivation layer was estimated to have suitable optical properties, and the photoresponse of photosensor was increased after the formation of passivation layer. The immobilization efficiency of a parylene-H film through covalent bonding was compared with the immobilization of Z-domains through physical adsorption. Finally, on-chip chemiluminescent immunoassay of bacteria was carried out by immobilizing antibodies against Escherichia coli through Z-domains for the orientation control of antibodies. The limit of detection was determined to be less than 104E. coli/mL (n=3), and the sensitivity of bacterial detection was estimated to be 0.339 pA/E. coli/mL (n=3) with a linearity factor (R2) of 0.999. These results showed that the on-chip chemiluminescent immunoassay for bacterial detection could be performed using passivation layer coated CdS NW photosensor.

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Acknowledgements

This work was supported by the National Research Foundation of Korea [grant number: NRF-2020R1A2B5B01002187].

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Authors and Affiliations

  1. Department of Materials Sciences and Engineering, Yonsei University, 50 Yonsei-Ro, Seo-dae-mun-gu, Seoul, 120-749, Korea

    Hong-Rae Kim, Ji-Hong Bong, Jaeyong Jung, Jeong Soo Sung & Jae-Chul Pyun

  2. Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 136-791, Korea

    Min-Jung Kang & Jae-Gwan Park

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  1. Hong-Rae Kim
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  2. Ji-Hong Bong
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  3. Jaeyong Jung
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Correspondence to Jae-Chul Pyun.

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Kim, HR., Bong, JH., Jung, J. et al. An On-chip Chemiluminescent Immunoassay for Bacterial Detection using in Situ-synthesized Cadmium Sulfide Nanowires with Passivation Layers. BioChip J 14, 268–278 (2020). https://doi.org/10.1007/s13206-020-4305-1

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  • DOI: https://doi.org/10.1007/s13206-020-4305-1

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