Issue 36, 2020
From the journal:

Chemical Science

An integrated electrocatalytic nESI-MS platform for quantification of fatty acid isomers directly from untreated biofluids†

Kavyasree Chintalapudi ORCID logo a  and  Abraham K. Badu-Tawiah ORCID logo *a  

* Corresponding authors

a Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA
E-mail: badu-tawiah.1@osu.edu

Abstract

Positional isomers of alkenes are frequently transparent to the mass spectrometer and it is difficult to provide convincing data to support their presence. This work focuses on the development of a new reactive nano-electrospray ionization (nESI) platform that utilizes non-inert metal electrodes (e.g., Ir and Ru) for rapid detection of fatty acids by mass spectrometry (MS), with concomitant localization of the C[double bond, length as m-dash]C bond to differentiate fatty acid isomers. During the electrospray process, the electrical energy (direct current voltage) is harnessed for in situ oxide formation on the electrode surface via electro-oxidation. The as-formed surface oxides are found to facilitate in situ epoxide formation at the C[double bond, length as m-dash]C bond position and the products are analyzed by MS in real-time. This phenomenon has been applied to analyze isomers of unsaturated fatty acids from complex serum samples, without pre-treatment.

Graphical abstract: An integrated electrocatalytic nESI-MS platform for quantification of fatty acid isomers directly from untreated biofluids

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Article information

DOI
https://doi.org/10.1039/D0SC03403G
Article type
Edge Article
Submitted
18 Jun 2020
Accepted
23 Aug 2020
First published
25 Aug 2020
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2020,11, 9891-9897

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An integrated electrocatalytic nESI-MS platform for quantification of fatty acid isomers directly from untreated biofluids

K. Chintalapudi and A. K. Badu-Tawiah, Chem. Sci., 2020, 11, 9891 DOI: 10.1039/D0SC03403G

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