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BIOENERGETICS

Water route for proton pumping in mitochondrial complex I

Nature Structural & Molecular Biology volume 27, pages 1–3 (2020)Cite this article

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A new cryo-EM structure of mitochondrial complex I reveals ordered water molecules that connect key elements of the proton-translocating machinery. Analysis of the ubiquinone-binding site of complex I offers insights into the mechanism of catalytic turnover and the regulation of this essential metabolic enzyme.

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Fig. 1: The Yarrowia lipolytica complex I structure determined by Grba and Hirst (PDB 6YJ4).

References

  1. Grba, D. & Hirst, J. Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-020-0473-x (2020).

  2. Kotlyar, A. B. & Vinogradov, A. D. Biochim. Biophys. Acta 1019, 151–158 (1990).

    Article  CAS  Google Scholar 

  3. Dröse, S., Stepanova, A. & Galkin, A. Biochim. Biophys. Acta 1857, 946–957 (2016).

    Article  Google Scholar 

  4. Chouchani, E. T. et al. Nat. Med. 19, 753–759 (2013).

    Article  CAS  Google Scholar 

  5. Sazanov, L. A. & Hinchliffe, P. Science 311, 1430–1436 (2006).

    Article  CAS  Google Scholar 

  6. Zickermann, V. et al. Science 347, 44–49 (2015).

    Article  CAS  Google Scholar 

  7. Hunte, C., Zickermann, V. & Brandt, U. Science 329, 448–451 (2010).

    Article  CAS  Google Scholar 

  8. Parey, K. et al. Elife 7, e39213 (2018).

    Article  Google Scholar 

  9. Baradaran, R., Berrisford, J. M., Minhas, G. S. & Sazanov, L. A. Nature 494, 443–448 (2013).

    Article  CAS  Google Scholar 

  10. Efremov, R. G. & Sazanov, L. A. Nature 476, 414–420 (2011).

    Article  CAS  Google Scholar 

  11. Haapanen, O. & Sharma, V. Sci. Rep. 7, 7747 (2017).

    Article  Google Scholar 

  12. Di Luca, A., Gamiz-Hernandez, A. P. & Kaila, V. R. I. Proc. Natl Acad. Sci. USA 114, E6314–E6321 (2017).

    Article  Google Scholar 

  13. Agip, A.-N. A. et al. Nat. Struct. Mol. Biol. 25, 548–556 (2018).

    Article  CAS  Google Scholar 

  14. Brandt, U. Biochim. Biophys. Acta 1807, 1364–1369 (2011).

    Article  CAS  Google Scholar 

  15. Verkhovskaya, M. L., Belevich, N., Euro, L., Wikström, M. & Verkhovsky, M. I. Proc. Natl Acad. Sci. USA 105, 3763–3767 (2008).

    Article  CAS  Google Scholar 

  16. Kaila, V. R. I. J. R. Soc. Interface 15, 20170916 (2018).

    Article  Google Scholar 

  17. Dröse, S. et al. PLoS Biol. 9, e1001128 (2011).

    Article  Google Scholar 

  18. Babot, M., Birch, A., Labarbuta, P. & Galkin, A. Biochim. Biophys. Acta 1837, 1083–1092 (2014).

    Article  CAS  Google Scholar 

  19. Loskovich, M. V., Grivennikova, V. G., Cecchini, G. & Vinogradov, A. D. Biochem. J. 387, 677–683 (2005).

    Article  CAS  Google Scholar 

Download references

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  1. Department of Pediatrics, Columbia University, New York, NY, USA

    Alexander Galkin

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  1. Alexander Galkin
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Correspondence to Alexander Galkin.

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Galkin, A. Water route for proton pumping in mitochondrial complex I. Nat Struct Mol Biol 27, 1–3 (2020). https://doi.org/10.1038/s41594-020-0500-y

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