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Enzymatic Esterification under High-pressure CO2 Conditions for in situ Recovery of Butyric Acid from Anaerobic Fermenters

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  • Enzyme Biotechnology
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Abstract

Butyric acid, a short chain carboxylic acid with diverse usages, is produced by Clostridia fermentation. In the industrial scale production of butyric acid, its separation and recovery from fermentation broth requires energy-intensive processes. To reduce the product recovery costs, it is necessary to convert butyric acid into a chemical with a much higher partition coefficient in the hydrophobic extractants than butyric acid. Butyl butyrate, with an excellent partition coefficient to tetradecane, can be produced by the enzymatic conversion of butyric acid via esterification. Moreover, butyl butyrate can be used as a valuable fuel source and additive in the food, cosmetic, and pharmaceutical industries. Novozyme 435, Candida antarctica lipase B immobilized on acrylic resin, and tetradecane were used as the enzyme and extractant, respectively. A high-pressure CO2-facilitated reactor was used to temporarily drop the pH of the fermentation broth so that the enzymatic reaction could be activated. The in situ removal of butyric acid and simultaneous production of butyl butyrate were processed continuously during fermentation. To optimize the enzymatic reaction, it was necessary to maintain a temperature of 40°C at 50 bar and an optimal molar ratio of substrate. In the extractive fermentation, 11.6 g/L butyl butyrate was produced with a productivity of 0.77 g/L/h from butyrate produced through fermentation. This process is expected to be able to extract carboxylic acids with more diverse carbon lengths in ester form.

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Acknowledgements

This work was supported in part by a grant funded by Hanyang University in the Republic of Korea (HY-201100000000233-N) and by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea (No. 20173010092510). The authors declare no conflict of interest. Neither ethical approval nor informed consent was required for this study.

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  1. Department of Chemical Engineering, Hanyang University, Seoul, 04763, Korea

    Jaesung Chun & Byoung-In Sang

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  1. Jaesung Chun
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Chun, J., Sang, BI. Enzymatic Esterification under High-pressure CO2 Conditions for in situ Recovery of Butyric Acid from Anaerobic Fermenters. Biotechnol Bioproc E 25, 616–622 (2020). https://doi.org/10.1007/s12257-020-0158-7

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  • DOI: https://doi.org/10.1007/s12257-020-0158-7

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