Skip to content
Licensed Unlicensed Requires Authentication Published by De Gruyter August 21, 2020

Effect of pH on xylitol production by Candida species from a prairie cordgrass hydrolysate

  • Samatha S. R. Rudrangi and Thomas P. West EMAIL logo

Abstract

Using hydrolysates of the North American prairie grass prairie cordgrass buffered at pH 4.5, 5.0, 5.5 or 6.0, xylitol production, xylitol yield, cell biomass production and productivity were investigated for three strains of yeast Candida. Of the three strains, the highest xylitol concentration of 20.19 g xylitol (g xylose consumed)−1 and yield of 0.89 g xylitol (g xylose consumed)−1 were produced by Candida mogi ATCC 18364 when grown for 120 h at 30° C on the pH 5.5-buffered hydrolysate-containing medium. The highest biomass level being 7.7 g cells (kg biomass)−1 was observed to be synthesized by Candida guilliermondii ATCC 201935 after 120 h of growth at 30° C on a pH 5.5-buffered hydrolysate-containing medium. The highest xylitol specific productivity of 0.73 g xylitol (g cells h)−1 was determined for C. guilliermondii ATCC 20216 after 120 h of growth at 30°C on a pH 5.0-buffered hydrolysate-containing medium. Xylitol production and yield by the three Candida strains was higher on prairie cordgrass than what was previously observed for the same strains after 120 h at 30° C when another North American prairie grass big bluestem served as the plant biomass hydrolysate indicating that prairie cordgrass may be a superior plant biomass substrate.


Corresponding author: Thomas P. West, Department of Chemistry, Texas A&M University-Commerce, Commerce, 75429, TX, USA, E-mail:

Funding source: Welch Foundation

Award Identifier / Grant number: T-0014

  1. Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: Financial support of this work was provided by the Welch Foundation Grant T-0014.

  3. Competing interests: The authors declare no conflicts of interest regarding this article.

References

1. Gränstrom, TB, Izumori, K, Leisola, M. A rare sugar xylitol. Part II: biotechnological production and future applications of xylitol. Appl Microbiol Biotechnol 2007;74:273–6. https://doi.org/10.1007/s00253-006-0760-4.Search in Google Scholar PubMed

2. Ur-Rehman, S, Mushtaq, Z, Zahoor, T, Jamil, A, Murtaza, MA. Xylitol: a review on bioproduction, application, health benefits, and related safety issues. Crit Rev Food Sci Nutr 2015;55:1514–28. https://doi.org/10.1080/10408398.2012.702288.Search in Google Scholar PubMed

3. Petch, D, Butler, M. The effect of alternative carbohydrates on the growth and antibody production of a murine hybridoma. Appl Biochem Biotechnol 1996;59:93–104. https://doi.org/10.1007/bf02787861.Search in Google Scholar

4. Uhari, M, Kontiokari, T, Niemela, M. A novel use of xylitol sugar in preventing acute otitis media. Pediatrics 1988;102:879–84. https://doi.org/10.1542/peds.102.4.879.Search in Google Scholar PubMed

5. Silva, SS, Matos, ZR, Carvalho, W. Effects of sulfuric acid loading and resident time on the composition of sugarcane bagasse hydrolysate and its use as a source of xylose for xylitol bioproduction. Biotechnol Prog 2005;21:1449–52. https://doi.org/10.1021/bp0502025.Search in Google Scholar PubMed

6. Zhang, Y-HP. Reviving the carbohydrate economy via multi-product lignocellulose biorefineries. J Ind Microbiol Biotechnol 2008;35:367–75. https://doi.org/10.1007/s10295-007-0293-6.Search in Google Scholar PubMed

7. Mulkey, VR, Owens, VN, Lee, DK. Management of warm-season grass mixtures for biomass production in South Dakota USA. Bioresour Technol 2008;99:609–17. https://doi.org/10.1016/j.biortech.2006.12.035.Search in Google Scholar PubMed

8. Kennedy, DEII, West, TP. Effect of yeast extract addition to a mineral salts medium containing hydrolyzed plant xylan on fungal pullulan production. Z Naturforsch C 2018;73:319–23. https://doi.org/10.1515/znc-2018-0018.Search in Google Scholar PubMed

9. Garrote, G, Dominguez, H, Parajo, JC. Manufacture of xylose-based fermentation media from corncobs by posthydrolysis of autohydrolysis liquors. Appl Biochem Biotechnol 2001;95:195–207. https://doi.org/10.1385/abab:95:3:195.10.1385/ABAB:95:3:195Search in Google Scholar

10. Onishi, H, Suzuki, T. Microbial production of xylitol from glucose. Appl Microbiol 1969;18:1031–5. https://doi.org/10.1128/aem.18.6.1031-1035.1969.Search in Google Scholar

11. Gong, C-S, Claypool, TA, McCracken, LD, Maun, CM, Ueng, PP, Tsao, GT. Conversion of pentoses by yeasts. Biotechnol Bioeng 1983;25:85–102. https://doi.org/10.1002/bit.260250108.Search in Google Scholar PubMed

12. Barbosa, MFS, de Medeiros, MB, de Mancilha, IM, Schneider, H, Lee, H. Screening of yeasts for production of xylitol from D-xylose and some factors which affect xylitol yield in Candida guilliermondii. J Ind Microbiol 1988;3:241–51. https://doi.org/10.1007/bf01569582.Search in Google Scholar

13. Tochampa, W, Sirisansaneeyakul, S, Vanichsriratana, W, Srinophakun, P, Bakker, HHC, Chisti, Y. A model of xylitol production by the yeast Candida mogii. Bioproc Biosyst Eng 2005;28:175–83. https://doi.org/10.1007/s00449-005-0025-0.Search in Google Scholar PubMed

14. Guo, C, Zhao, C, He, P, Shen, A, Jiang, N. Screening and characterization of yeasts for xylitol production. J Appl Microbiol 2006;101:1096–104. https://doi.org/10.1111/j.1365-2672.2006.02994.x.Search in Google Scholar PubMed

15. Cortez, DV, Roberto, IC. Effect of phosphate buffer concentration on the batch xylitol production by Candida guilliermondii. Lett Appl Microbiol 2006;42:321–5. https://doi.org/10.1111/j.1472-765x.2006.01864.x.Search in Google Scholar PubMed

16. Wannawilaia, S, Sirisansaneeyakula, S, Chisti, Y. Benzoate-induced stress enhances xylitol yield in aerobic fed-batch culture of Candida mogii TISTR 5892. J Biotechnol 2015;194:58–66. https://doi.org/10.1016/j.jbiotec.2014.11.037.Search in Google Scholar PubMed

17. Mayerhoff, ZDVL, Roberto, IC, Silva, SS. Xylitol production from rice straw hemicellulose using different yeast strains. Biotechnol Lett 1997;19:407–9. https://doi.org/10.1023/a:1018375506584.10.1023/A:1018375506584Search in Google Scholar

18. Silva, SS, Ribeiro, JD, Felipe, MGA, Vitolo, M. Maximizing the xylitol production from sugar cane bagasse hydrolysate by controlling the aeration rate. Appl Biochem Biotechnol 1997;63-65:558–63. https://doi.org/10.1007/978-1-4612-2312-2_47.Search in Google Scholar

19. Altamirano, A, Vazquez, F, de Figueroa, LIC. Isolation and identification of xylitol-producing yeasts from agricultural residues. Folia Microbiol 2000;45:255–8. https://doi.org/10.1007/bf02908955.Search in Google Scholar

20. Rodrigues, RCLB, Felipe, MGA, Roberto, IC, Vitolo, M. Batch xylitol production by Candida guilliermondii FTI 20037 from sugarcane bagasse hemicellulosic hydrolyzate at controlled pH values. Bioproc Biosyst Eng 2003;26:103–7. https://doi.org/10.1007/s00449-003-0332-2.Search in Google Scholar PubMed

21. Mussatto, SI, Santo, JC, Roberto, IC. Effect of pH and activated charcoal adsorption on hemicellulosic hydrolysate detoxification for xylitol production. J Chem Technol Biotechnol 2004;79:590–6. https://doi.org/10.1002/jctb.1026.Search in Google Scholar

22. Rodrigues, RCLB, Sene, L, Matos, GS, Roberto, IC, PessoaJr., Felipe, MGA. Enhanced xylitol production by precultivation of Candida guilliermondii cells in sugarcane bagasse hemicellulosic hydrolysate. Curr Microbiol 2006;53:53–9. https://doi.org/10.1007/s00284-005-0242-4.Search in Google Scholar PubMed

23. Gurpilhares, DB, Hasmann, FA, Pessoa, AJr, Roberto, IC. The behavior of key enzymes of xylose metabolism on the xylitol production by Candida guilliermondii grown in hemicellulosic hydrolysate. J Ind Microbiol Biotechnol 2009;36:87–93. https://doi.org/10.1007/s10295-008-0475-x.Search in Google Scholar PubMed

24. West, TP. Xylitol production by Candida species grown on a grass hydrolysate. World J Microbiol Biotechnol 2009;25:913–6. https://doi.org/10.1007/s11274-008-9947-4.Search in Google Scholar

25. Sene, L, Arruda, PV, Oliveira, SMM, Felipe, MGA. Evaluation of sorghum straw hemicellulosic hydrolysate for biotechnological production of xylitol by Candida guilliermondii. Braz J Microbiol 2011;42:1141–6. https://doi.org/10.1590/s1517-83822011000300036.Search in Google Scholar

26. Hernández-Pérez, AF, Arruda, PV, Felipe, MGA. Sugarcane straw as a feedstock for xylitol production by Candida guilliermondii FTI 20037. Braz J Microbiol 2016;47:489–96. https://doi.org/10.1016/j.bjm.2016.01.019.Search in Google Scholar PubMed PubMed Central

27. López-Linaresa, JC, Romeroa, I, Caraa, C, Castroa, E, Mussatto, SI. Xylitol production by Debaryomyces hansenii and Candida guilliermondii from rapeseed straw hemicellulosic hydrolysate. Bioresour Technol 2018;247:736–43. https://doi.org/10.1016/j.biortech.2017.09.139.Search in Google Scholar PubMed

28. West, TP. Fungal production of the polysaccharide pullulan from a plant hydrolysate. Z Naturforsch C 2017;72:491–5. https://doi.org/10.1515/znc-2017-0032.Search in Google Scholar PubMed

Received: 2020-06-16
Accepted: 2020-07-14
Published Online: 2020-08-21
Published in Print: 2020-11-26

© 2020 Walter de Gruyter GmbH, Berlin/Boston

Downloaded on 29.3.2024 from https://www.degruyter.com/document/doi/10.1515/znc-2020-0140/html
Scroll to top button