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Molecular Identification and In Vitro Plant Growth-Promoting Activities of Culturable Potato (Solanum tuberosum L.) Rhizobacteria in Tanzania

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Abstract

The present study investigated the diversity of culturable rhizobacteria associated with potato (S. tuberosum L.) in Tanzania and assessed their in vitro plant growth-promoting (PGP) activities to deduce their potential as biofertilizers. Potato rhizosphere soil and tuber samples (54 samples in total) were collected from 9 villages in three different agro-ecological regions in Tanzania. A total of 145 rhizobacterial isolates were obtained, 52 of which were selected and identified by partial 16S rRNA gene sequences and screened for various PGP traits in vitro including qualitative and quantitative solubilization of phosphorus (P), zinc (Zn) and potassium (K), nitrogen (N2) fixation and production of ammonia (NH3) in nitrogen-free medium, and indole-3-acetic acid (IAA), gibberellic acids (GA) and siderophores production. The results showed that the isolates were all Gammaproteobacteria, belonging to 4 families (Enterobacteriaceae, Yersiniaceae, Pseudomonadaceae and Morganellaceae) and 9 genera (Enterobacter, Klebsiella, Serratia, Pseudomonas, Morganella, Buttiauxella, Pantoea and Cedecea). Significant differences (P < 0.05) were observed for all assessed PGP abilities of the external and endophytic rhizobacterial isolates except for quantitative siderophore production and qualitative P and K solubilization for the external rhizobacteria and production of IAA and GA for the endophytic rhizobacteria. Among the best PGP isolates which can be exploited for biofertilization of the potato were Klebsiella pneumoniae KIBS1, K. grimontii LUTS10, Serratia liquefaciens KIBT1, Enterobacter ludwigii KIBS10 and Citrobacter freundii MWALS6. Comparative evaluation of PGP abilities of these two groups of isolates revealed significant differences (P < 0.05) only for NH3 and IAA production and qualitative K solubilization.

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Acknowledgements

We acknowledge Ms. Innes Fridrich and Dr. Amelie Dukunde for their invaluable support with methodologies throughout the molecular studies on the rhizobacterial isolates, and the Green Talents Programme of the Federal Ministry of Education and Research (BMBF) in Germany for the support offered by funding BNA’s research stay at the University of Gottingen which made it possible to complete this research.

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Authors and Affiliations

  1. Department of Sustainable Agriculture and Biodiversity Conservation, Nelson Mandela African Institution of Science and Technology, P. O. Box 447, Arusha, Tanzania

    B. N. Aloo & E. R. Mbega

  2. Department of Biological Sciences, University of Eldoret, P. O. Box 1125-30100, Eldoret, Kenya

    B. N. Aloo

  3. Department of Biological Sciences, Moi University, P.O. Box 3900-30100, Eldoret, Kenya

    E. R. Mbega & B. A. Makumba

  4. Institute of Microbiology and Genetics, Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Georg-August University of Göttingen, Grisebachstr. 8, 37077, Göttingen, Germany

    R. Hertel & R. Daniel

Authors
  1. B. N. Aloo
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  2. E. R. Mbega
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  3. B. A. Makumba
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  4. R. Hertel
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  5. R. Daniel
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Aloo B. N, Rolf D. and Hertel R. The first draft of this manuscript was written by Aloo B. N. and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to B. N. Aloo.

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Aloo, B.N., Mbega, E.R., Makumba, B.A. et al. Molecular Identification and In Vitro Plant Growth-Promoting Activities of Culturable Potato (Solanum tuberosum L.) Rhizobacteria in Tanzania. Potato Res. 64, 67–95 (2021). https://doi.org/10.1007/s11540-020-09465-x

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  • DOI: https://doi.org/10.1007/s11540-020-09465-x

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