Cellular internalization mechanism of novel Raman probes designed for plant cells

Keiko Midorikawa; Kousuke Tsuchiya; Simon Sau Yin Law; Yu Miyagi; Takuya Asai; Takanori Iino; Yasuyuki Ozeki; Yutaka Kodama; Keiji Numata

doi:10.1039/D0CB00128G

Cellular internalization mechanism of novel Raman probes designed for plant cells†

Keiko Midorikawa,‡^a Kousuke Tsuchiya,

‡*^ab Simon Sau Yin Law,^a Yu Miyagi,^a Takuya Asai,^c Takanori Iino,

^c Yasuyuki Ozeki,

^c Yutaka Kodama

*^ad and Keiji Numata

*^ab

Author affiliations

* Corresponding authors

^a Biomacromolecules Research Team, RIKEN Center for Sustainable Resource Science, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
E-mail: keiji.numata@riken.jp, kosuke.tsuchiya@riken.jp

^b Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto-Daigaku-Katsura, Nishikyo-ku, Kyoto 615-8510, Japan

^c Department of Electrical Engineering and Information Systems, The University of Tokyo, Tokyo 113-8656, Japan

^d Center for Bioscience Research and Education, Utsunomiya University, Tochigi 321-5805, Japan
E-mail: kodama@cc.utsunomiya-u.ac.jp

Abstract

Diphenylacetylene derivatives containing different polymeric components, poly(L-lysine) (pLys) or tetra(ethylene glycol) (TEG) were designed as novel Raman imaging probes with high Raman sensitivity and low cytotoxicity in living plant cells. The pLys-conjugated probe is internalized via an endocytosis-dependent pathway, whereas TEG-conjugated probe most likely induces direct penetration into the plant cells.

This article is part of the themed collection: Analytical methods in chemical biology

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DOI: https://doi.org/10.1039/D0CB00128G
Article type: Communication
Submitted: 17 Jul 2020
Accepted: 29 Jul 2020
First published: 20 Aug 2020
This article is Open Access

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RSC Chem. Biol., 2020,1, 204-208

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Cellular internalization mechanism of novel Raman probes designed for plant cells

K. Midorikawa, K. Tsuchiya, S. S. Y. Law, Y. Miyagi, T. Asai, T. Iino, Y. Ozeki, Y. Kodama and K. Numata, RSC Chem. Biol., 2020, 1, 204 DOI: 10.1039/D0CB00128G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

RSC Chemical Biology

Cellular internalization mechanism of novel Raman probes designed for plant cells†

Abstract

Supplementary files

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Cellular internalization mechanism of novel Raman probes designed for plant cells

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