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Bilayered nanosheets used for complex topography wound anti-infection

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Abstract

There is a consensus that the prevention of wound infection should be achieved in the following ways: (1) closing the wound to protect it from extra infection; (2) an antibacterial agent that could kill endogenous bacteria. However, existing bulk two-dimensional antibacterial materials show inefficient adhesion to wounds with complex morphology and thus cause the prevention of wound closure. Reducing the thickness of bulk two-dimensional materials to less than 100 nanometres endows them with great flexibility, which could allow them to adhere to wounds with complex morphology by only physical adhesion. Herein, a broad-spectrum and efficient antimicrobial peptide (AMP) was introduced to biocompatible methacrylated gelatine (GelMA) with multiple modification sites, which served as an inner antibacterial layer. After being combined with a biodegradable and good mechanical poly-l-lactide (PLLA) outer layer through plasma-treatment-assisted spin coating, we finally constructed bilayered antibacterial nanosheets with a thickness of approximately 80 nm. These bilayered nanosheets possess good adhesion to surfaces with complex topography and thus achieve better wound closure than other bulk two-dimensional materials. Moreover, this AMP-grafted conjugation shows minimal cytotoxicity compared with Ag+ antibacterial agents, and the antibacterial rate of nanosheets is dependent on the graft rate of AMP. We suggest that this bilayered antibacterial nanosheet might be an advanced anti-infection dressing for wound treatment in clinical settings.

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Data availability

The data used and analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We would like to thank the Shenzhen Science and Technology Program (JCYJ20170815153105076, GJHZ20180411143347603), the Guangdong Natural Science Funds for Distinguished Young Scholars (2016A030306018), the Science and Technology Program of Guangdong Province (2019B010941002, 2017B090911008), Outstanding Scholar Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory (2018GZR110102001), the National Nature Science Foundation of China (Grants U1801252) and the Science and Technology Program of Guangzhou (201804020060), the Science and Technology Program of Guangzhou (201804020060, 202007020002).

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Authors and Affiliations

  1. Peking University Shenzhen Institute, Peking University, Shenzhen, People’s Republic of China

    Chen Lai & Xuetao Shi

  2. School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510640, People’s Republic of China

    Chengkai Xuan, Xuemin Liu & Xuetao Shi

  3. National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, People’s Republic of China

    Chengkai Xuan, Xuemin Liu & Xuetao Shi

  4. Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, 510005, People’s Republic of China

    Chengkai Xuan, Xuemin Liu & Xuetao Shi

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  1. Chengkai Xuan
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  2. Xuemin Liu
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Contributions

Chengkai Xuan participated in the experimental research, data analysis, writing and editing of the manuscript. Xuemin Liu and Chen Lai performed the data analysis and the editing of the manuscript. Xuetao Shi performed the study design and editing of the manuscript. All authors have read and approved the final manuscript and, therefore, have full access to all the data in the study and take responsibility for the integrity and security of the data.

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Correspondence to Xuetao Shi.

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Xuan, C., Liu, X., Lai, C. et al. Bilayered nanosheets used for complex topography wound anti-infection. Bio-des. Manuf. 3, 373–382 (2020). https://doi.org/10.1007/s42242-020-00091-7

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  • DOI: https://doi.org/10.1007/s42242-020-00091-7

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