Abstract
A test of the sensitivity of seven colon cancer cell lines to a panel of 12 nonpathogenic human enteroviruses revealed significant differences in the ability of tumor cells to become infected and replicate different viral strains. Among the factors that can affect the sensitivity of cells to viruses are differences in the state of the mechanisms of antiviral protection, associated with a reaction to type I interferons. Using the two colon cancer cell lines CaCo2 and LIM1215 as a model, significant differences were revealed in the ability of cells to defend themselves against virus infection after 16 hours of treatment with 1000 units/mL of interferon-alpha. To study the effect of the state of the interferon response system, represented by the Jak/STAT signaling pathway, on the sensitivity of cells to different strains of enteroviruses, HEK293T cell lines were used. These are capable of supporting replication of each of the tested enteroviruses, as well as maintaining the ability to protect against viral infection after the treatment with interferon. Using the CRISPR/Cas9 system, HEK293T sublines with knockouts of the IFNAR1 and STAT2 genes were obtained. The sensitivity of control and knockout cells to infection with five strains of enteroviruses and the vesicular stomatitis virus was analyzed. It was noted that knockout of the IFNAR1 and STAT2 genes resulted in an increased sensitivity to all tested viruses. In knockout cells, the levels of reproduction of the vaccine dervied of poliovirus type 1, Echoviruses 7 and 30, and Coxsackie viruses B5 and A7 were also significantly increased in comparison with the control HEK293T cells. Thus, deficiencies in the Jak/STAT signaling pathway in tumor cells lead to an overall increase in the sensitivity to oncolytic viruses.
Similar content being viewed by others
REFERENCES
Lemay C.G., Keller B.A., Edge R.E., Abei M., Bell J.C. 2018. Oncolytic viruses: The best is yet to come. Curr. Cancer Drug Targets. 18, 109–123.
Lawler S.E., Speranza M.C., Cho C.F., Chiocca E.A. 2017. Oncolytic viruses in cancer treatment: A review. JAMA Oncol. 3, 841–849.
Fountzilas C., Patel S., Mahalingam D. 2017. Review: Oncolytic virotherapy, updates and future directions. Oncotarget. 8, 102617–102639.
Breitbach C.J., Lichty B.D., Bell J.C. 2016. Oncolytic viruses: Therapeutics with an identity crisis. EBioMedicine. 9, 31–36.
Matveeva O.V., Guo Z.-S., Shabalina S.V., Chumakov P.M. 2015. Oncolysis by paramyxoviruses: Multiple mechanisms contribute to therapeutic efficacy. Mol. Ther. Oncolyt. 2, 15011.
Bell J.C., McFadden G. 2015. Editorial overview: Oncolytic viruses-replicating virus therapeutics for the treatment of cancer. Curr. Opin. Virol. 13, viii-ix.
Chumakov P.M., Morozova V.V., Babkin I.V., Baikov I.K., Netesov S.V., Tikunova N.V. 2012. Oncolytic enteroviruses. Mol. Biol. (Moscow). 46 (5), 639–650.
Pallansch M., Roos R. 2007. Enteroviruses: Polioviruses, coxsackieviruses, echoviruses, and newer enteroviruses. In Fields Virology. Knipe D.M., Howley P.M. Eds. Philadelphia: Lippincott Williams and Wilkins, 840–893.
Stark G.R., Darnell J.E., Jr. 2012. The JAK-STAT pathway at twenty. Immunity. 36, 503–514.
Borden E.C., Sen G.C., Uze G., Silverman R.H., Ransohoff R.M., Foster G.R., Stark G.R. 2007. Interferons at age 50: Past, current and future impact on biomedicine. Nat. Rev. Drug Discov. 6, 975–990.
Groner B., von Manstein V. 2017. Jak Stat signaling and cancer: Opportunities, benefits and side effects of targeted inhibition. Mol. Cell. Endocrinol. 451, 1–14.
Matveeva O.V., Chumakov P.M. 2018. Defects in interferon pathways as potential biomarkers of sensitivity to oncolytic viruses. Rev. Med. Virol. e2008.
Pikor L.A., Bell J.C., Diallo J.-S. 2015. Oncolytic viruses: Exploiting cancer’s deal with the Devil. Trends Cancer. 1, 266–277.
Heiber J.F., Barber G.N. 2012. Evaluation of innate immune signaling pathways in transformed cells. Methods Mol. Biol. 797, 217–238.
Naik S., Russell S.J. 2009. Engineering oncolytic viruses to exploit tumor specific defects in innate immune signaling pathways. Exp. Opin. Biol. Ther. 9, 1163–1176.
Stojdl D.F., Lichty B., Knowles S., Marius R., Atkins H., Sonenberg N., Bell J.C. 2000. Exploiting tumor-specific defects in the interferon pathway with a previously unknown oncolytic virus. Nat. Med. 6, 821–825.
Yoneyama M., Kikuchi M., Matsumoto K., Imaizumi T., Miyagishi M., Taira K., Foy E., Loo Y.M., Gale M., Jr., Akira S., Yonehara S., Kato A., Fujita T. 2005. Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity. J. Immunol. 175, 2851–2858.
Novick D., Cohen B., Rubinstein M. 1994. The human interferon alpha/beta receptor: Characterization and molecular cloning. Cell. 77, 391–400.
Heim M.H. 1999. The Jak-STAT pathway: Cytokine signalling from the receptor to the nucleus. J. Receptor Signal Transduct. Res. 19, 75–120.
Fu X.Y., Kessler D.S., Veals S.A., Levy D.E., Darnell J.E., Jr. 1990. ISGF3, the transcriptional activator induced by interferon alpha, consists of multiple interacting polypeptide chains. Proc. Natl. Acad. Sci. U. S. A.87, 8555–8559.
Kessler D.S., Levy D.E., Darnell J.E., Jr. 1988. Two interferon-induced nuclear factors bind a single promoter element in interferon-stimulated genes. Proc. Natl. Acad. Sci. U. S. A.85, 8521–8525.
de Veer M.J., Holko M., Frevel M., Walker E., Der S., Paranjape J.M., Silverman R.H., Williams B.R. 2001. Functional classification of interferon-stimulated genes identified using microarrays. J. Leukoc. Biol. 69, 912–920.
Whithead R.H., Nice E.C., Lloyd C.J., James R., Burgess A.W. 1990. Detection of colonic growth factors using a human colonic carcinoma cell line (LIM1215). Int. J. Cancer. 46, 858–863.
Reed L.J., Muench H. 1938. A simple method of estimating fifty per cent endpoints. Am. J. Hygiene. 27, 493–497.
Funding
The study was supported by the Russian Foundation for Basic Research (project no. 18-29-01059) and the Center for Strategic Planning and Assessment of Biomedical Health Risks of the Federal Midical-Biological Agency of the Russian Federation (grant no. 1.1597).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
Rights and permissions
About this article
Cite this article
Le, T.H., Lipatova, A.V., Volskaya, M.A. et al. The State of The Jak/Stat Pathway Affects the Sensitivity of Tumor Cells to Oncolytic Enteroviruses. Mol Biol 54, 570–577 (2020). https://doi.org/10.1134/S002689332004010X
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S002689332004010X