Abstract
Estimating the presence and abundance of non-native species in the early stage of invasion is important to prevent further spread of non-native species in aquatic systems. Environmental DNA (eDNA) methods have been applied for aquatic plants; however, it is still questionable on the accuracy of the eDNA methods for the detection and abundance estimation of aquatic plants in lotic systems. Here, we studied the invasive aquatic plant Egeria densa in the tributaries of the Gonokawa River in Japan to compare the E. densa detection rate of the eDNA method with that of the three sampling methods and examine the relationships of the eDNA flux to the upstream plant coverage, plant stem fragment flux, and plant tissue DNA flux of E. densa in the study sites on each season. Our results indicated that the eDNA method better detected the presence of E. densa in our study sites than other methods. The eDNA flux was directly related to the coverage rank and the plant tissue DNA flux in the study sites in both months. These results suggest that the eDNA methods have a potential as monitoring tool for early detection and relative abundance estimation of invasive aquatic plants in lotic systems.
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References
Anglès d’Auriac MB, Strand DA, Mjelde M, Demars BOL, Thaulow J (2019) Detection of an invasive aquatic plant in natural water bodies using environmental DNA. PLoS ONE 14:e0219700. https://doi.org/10.1371/journal.pone.0219700
Buxton AS, Groombridge JJ, Griffiths RA (2018) Seasonal variation in environmental DNA detection in sediment and water samples. PLoS ONE 13:e0191737. https://doi.org/10.1371/journal.pone.0191737
Core Team R (2019) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna
Deiner K, Altermatt F (2014) Transport distance of invertebrate environmental DNA in a natural river. PLoS ONE 9:e88786. https://doi.org/10.1371/journal.pone.0088786
Doi H, Inui R, Akamatsu Y, Kanno K, Yamanaka H, Takahara T, Minamoto T (2017) Environmental DNA analysis for estimating the abundance and biomass of stream fish. Freshw Biol 62:30–39. https://doi.org/10.1111/fwb.12846
Fujiwara A, Matsuhashi S, Doi H, Yamamoto S, Minamoto T (2016) Use of environmental DNA to survey the distribution of an invasive submerged plant in ponds. Freshw Sci 35:748–754. https://doi.org/10.1086/685882
Fukumoto S, Ushimaru A, Minamoto T (2015) A basin-scale application of environmental DNA assessment for rare endemic species and closely related exotic species in rivers: a case study of giant salamanders in Japan. J Appl Ecol 52:358–365. https://doi.org/10.1111/1365-2664.12392
Haramoto T, Ikushima I (1988) Life cycle of Egeria densa Planch., an aquatic plant naturalized in Japan. Aquat Bot 30:389–403. https://doi.org/10.1016/0304-3770(88)90070-8
Hervé M (2020) RVAideMemoire: testing and plotting procedures for biostatistics. R package version 0.9–75. https://cran.rproject.org/web/packages/RVAideMemoire/index.html
Inui R, Goto M, Kono T, Akamatsu Y, Kakenami Y, Hitotsumatsu A (2017) Environmental DNA analysis for estimating the resources of Plecoglossus altivelis in Gonokawa River. J Jpn Soc Civ Eng Ser B1 (Hydraulic Engineering) 73:1105–1110 (in Japanese)
Jo T, Fukuoka A, Uchida K, Ushimaru A, Toshifumi M (2020) Multiplex real-time PCR enables the simultaneous detection of environmental DNA from freshwater fishes: a case study of three exotic and three threatened native fishes in Japan. Biol Invasions 22:455–471. https://doi.org/10.1007/s10530-019-02102-w
Kadono Y (2004) Alien aquatic plants naturalized in Japan: history and present status. Glob Environ Res 8:163–169
Kodama T, Akamatsu Y, Miyazono S, Yamaguchi K (2019) Examination of washout characteristic of Egeria densa during large flood. Proc Hydraulic Eng 75:391–396 (in Japanese)
Matsuhashi S, Doi H, Fujiwara A, Watanabe S, Minamoto T (2016) Evaluation of the environmental DNA method for estimating distribution and biomass of submerged aquatic plants. PLoS ONE 11:e0156217. https://doi.org/10.1371/journal.pone.0156217
Matsuhashi S, Minamoto T, Doi H (2019) Seasonal change in environmental DNA concentration of a submerged aquatic plant species. Freshw Sci 38:654–660. https://doi.org/10.1086/704996
Matthews J, Koopman KR, Beringen R, Ode B, Pot R, van der Velde G, van Valkenburg JLCH, Leuven RSEW (2014) Knowledge document for risk analysis of the nonnative brazilian waterweed (Egeria densa) in the Netherlands. Radboud University, Nijmegen
Miyazono S, Taylor CM (2016) Fish species incidence patterns in naturally fragmented Chihuahuan desert streams. Ecol Freshw Fish 25:545–552. https://doi.org/10.1111/eff.12232
Scrivera M, Marinichb A, Wilsonc C, Freelanda J (2015) Development of species specific environmental DNA (eDNA) markers for invasive aquatic plants. Aquat Bot 122:27–31. https://doi.org/10.1016/j.aquabot.2015.01.003
Takahara T, Minamoto T, Yamanaka H, Doi H, Kawabata Z (2012) Estimation of fish biomass using environmental DNA. PLoS ONE 7:e35868. https://doi.org/10.1371/journal.pone.0035868
Tanaka S, Tsubaki R, Kawahara Y, Kotegawa Y (2016) Flushing flow impact on invasive aquatic plant in a reach downstream of a dam. J Jpn Soc Civ Eng Ser B1(72):1117–1122 (in Japanese)
Uchii K, Doi H, Minamoto T (2016) A novel environmental DNA approach to quantify the cryptic invasion of non-native genotypes. Mol Ecol Resour 16:415–422. https://doi.org/10.1111/1755-0998.12460
Yamanaka H, Minamoto T, Matsuura J, Sakurai S, Tsuji S, Motozawa H, Hongo M, Sogo Y, Kakimi N, Teramura I, Sugita M, Baba M, Kondo A (2017) A simple method for preserving environmental DNA in water samples at ambient temperature by addition of cationic surfactant. Limnol 18:233–241. https://doi.org/10.1007/s10201-016-0508-5
Yarrow M, Marín VH, Finlayson M, Tironi A, Delgado LE, Fischer F (2009) The ecology of Egeria densa Planchon (Liliopsida: Alismatales): a wetland ecosystem engineer? Revista Chilena de Historia Natural 82:299–313
Acknowledgements
We thank T. Kono, K. Yamaguchi, and N. Onaka for helping our field sampling. We also thank H. Fujii, M. Amimoto, K. Kobayashi, Y. Sugita, and M. Taguchi for the laboratory work. This research was supported by Chugoku Regional Development Bureau of Ministry of Land, Infrastructure, Transport and Tourism.
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Miyazono, S., Kodama, T., Akamatsu, Y. et al. Application of environmental DNA methods for the detection and abundance estimation of invasive aquatic plant Egeria densa in lotic habitats. Limnology 22, 81–87 (2021). https://doi.org/10.1007/s10201-020-00636-w
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DOI: https://doi.org/10.1007/s10201-020-00636-w