A lysosome-targeting viscosity-sensitive fluorescent probe based on a novel functionalised near-infrared xanthene-indolium dye and its application in living cells

Chang Liu; Tong Zhao; Song He; Liancheng Zhao; Xianshun Zeng

doi:10.1039/D0TB01329C

A lysosome-targeting viscosity-sensitive fluorescent probe based on a novel functionalised near-infrared xanthene-indolium dye and its application in living cells†

Chang Liu,^ab Tong Zhao,^ab Song He,^ab Liancheng Zhao^abc and Xianshun Zeng

*^ab

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* Corresponding authors

^a Tianjin Key Laboratory for Photoelectric Materials and Devices, School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, China
E-mail: xshzeng@tjut.edu.cn

^b Key Laboratory of Display Materials and Photoelectric Devices, Ministry of Education, School of Materials Science & Engineering, Tianjin University of Technology, Tianjin, China

^c School of Materials Science and Engineering, Institute of Information Functional Materials& Devices, Harbin Institute of Technology, Harbin, China

Abstract

The viscosity of lysosomes plays a significant role in modulating biological processes and reflects the status and function of this kind of organelle, e.g., locations, morphologies, and components. Herein, we constructed a novel near-infrared (NIR) lysosome-targeting viscosity probe, Lyso-cy, for monitoring viscosity changes in biological systems. The Lyso-cy probe showed very strong fluorescence emission at around 710 nm in viscous media. The fluorescence intensity of Lyso-cy increased 122-fold from when in water to when in 95% glycerol. Moreover, Lyso-cy proved to be an ideal lysosome-targeting tracer for monitoring fluctuations in the viscosity of a living cell with high spatial and temporal resolution under laser confocal microscopy.

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DOI: https://doi.org/10.1039/D0TB01329C
Article type: Paper
Submitted: 26 May 2020
Accepted: 17 Aug 2020
First published: 18 Aug 2020

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J. Mater. Chem. B, 2020,8, 8838-8844

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A lysosome-targeting viscosity-sensitive fluorescent probe based on a novel functionalised near-infrared xanthene-indolium dye and its application in living cells

C. Liu, T. Zhao, S. He, L. Zhao and X. Zeng, J. Mater. Chem. B, 2020, 8, 8838 DOI: 10.1039/D0TB01329C

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Journal of Materials Chemistry B

A lysosome-targeting viscosity-sensitive fluorescent probe based on a novel functionalised near-infrared xanthene-indolium dye and its application in living cells†

Abstract

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A lysosome-targeting viscosity-sensitive fluorescent probe based on a novel functionalised near-infrared xanthene-indolium dye and its application in living cells

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