Abstract
Drought stress is the most important limiting factor of crop cultivation around the world and stress conditions will modulate the expression of many genes that play a significant role in the plant growth and development and to stress adaptation. The apoplast is a dynamic environment subject and considered as a ‘key information bridge’ between the environment and cells. In the present study we aimed to measure the changes in the expression levels of genes by RT-qPCR studies.Analysis shown the down-regulation of Peptidylprolyl isomerase and Pin II type protease inhibitor and there observed the huge increase in the expression of Pectinesterase and Alpha L fucosidase under treated conditions. These changes in the expression level of proteins under stress conditions implicate their possible role in stress tolerance and would prove to be useful for protein phenotyping.
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Abbreviations
- RT-qPCR:
-
Quantitative reverse transcript polymerase chain reaction
- LC-MS:
-
Liquid chromatography coupled with mass spectrometry
- DNA:
-
Deoxyribonuclic acid
- cDNA:
-
Complementary DeoxyriboNuclic Acid
- RNA:
-
Ribonuclic Acid
- NCBI:
-
National Center for Biotechnology Information
- NaCl:
-
Sodium chloride
- DEPC water:
-
Diethyl pyrocarbonate
- 2-DDCT method:
-
2-∆∆Cycle threshold
- UBI-3:
-
Ubiquitin
References
Agrawal GK, Jwa NS, Lebrun MH, Job D, Rakwal R (2010) Plant secretome: unlocking secrets of the secreted proteins. Proteomics 10:799–827. https://doi.org/10.1002/pmic.200900514
Augur C, Stiefel V, Darvill A, Albersheim P, Puigdomenech P (1995) Molecular Cloning and Pattern of Expression of an α-L-Fucosidase Gene from Pea Seedlings. J Biol Chem 270:24839–24843. https://doi.org/10.1074/jbc.270.42.24839
Balasubramanian V, Vashisht D, Cletus J, Sakthivel N (2012) Plant β-1,3-glucanases: their biological functions and transgenic expression against phytopathogenic fungi. Biotechnol Lett 34:1983–1990. https://doi.org/10.1007/s10529-012-1012-6
Cruz de Carvalho MH, d’Arcy-Lameta A, Roy-Macauley H, Gareil M, El Maarouf H, Pham-Thi AT, Zuily-Fodil Y (2001) Aspartic protease in leaves of common bean (Phaseolus vulgaris L.) and cowpea (Vigna unguiculata L. Walp): enzymatic activity, gene expression and relation to drought susceptibility. FEBS Lett 492:242–246. https://doi.org/10.1016/S0014-5793(01)02259-1
De la Torre F, Sampedro J, Zarra I, Revilla G (2002) AtFXG1, an Arabidopsis gene encoding α-L-fucosidase active against fucosylated xyloglucan oligosaccharides. Plant Physiol 128:247–255. https://doi.org/10.1104/pp.010508
De Oliveira Carvalho A, Moreira Gomes V (2011) Plant defensins and defensin-like peptides-biological activities and biotechnological applications. Curr Pharm Des 17:4270–4293. https://doi.org/10.2174/138161211798999447
Do HM, Lee SC, Jung HW, Sohn KH, Hwang BK (2004) Differential expression and in situ localization of a pepper defensin (CADEF1) gene in response to pathogen infection, abiotic elicitors and environmental stresses in Capsicum annuum. Plant Sci 166:1297–1305. https://doi.org/10.1016/j.plantsci.2004.01.008
El-Tayeb MA, Ahmed MK (2007) Apoplastic protein pattern, hydrolases and peroxidase activity of Vicia faba cultivars as influenced by drought. Int J Agric Biol 09:226–230. http://www.fspublishers.org/published_papers/14162_..pdf
Faghani E, Gharechahi J, Komatsu S, Mirzaei M, Khavarinejad RA, Najafi F, Farsad LK, Salekdeh GH (2015) Comparative physiology and proteomic analysis of two wheat genotypes contrasting in drought tolerance. J Proteomics 114:1–15. https://doi.org/10.1016/j.jprot.2014.10.018
Foyer CH, Rasool B, Davey JW, Hancock RD (2016) Cross-tolerance to biotic and abiotic stresses in plants: a focus on resistance to aphid infestation. J Exp Bot 67:2025–2037. https://doi.org/10.1093/jxb/erw079
Güler NS, Sağlam A, Demiralay M, Kadioğlu A (2012) Apoplastic and symplastic solute concentrations contribute to osmotic adjustment in bean genotypes during drought stress. Turkish J Biol 36:151–160. https://doi.org/10.3906/biy-1101-177
Hachez C, Moshelion M, Zelazny E, Cavez D, Chaumont F (2006) Localization and quantification of plasma membrane aquaporin expression in maize primary root: a clue to understanding their role as cellular plumbers. Plant Mol Biol 62:305–323. https://doi.org/10.1007/s11103-006-9022-1
Huang Y, Xiao B, Xiong L (2007) Characterization of a stress responsive proteinase inhibitor gene with positive effect in improving drought resistance in rice. Planta 226:73–85. https://doi.org/10.1007/s00425-006-0469-8
Iovieno P, Punzo P, Guida G, Mistretta C, Van Oosten MJ, Nurcato R, Bostan H, Colantuono C, Costa A, Bagnaresi P, Chiusano ML (2016) Transcriptomic changes drive physiological responses to progressive drought stress and rehydration in tomato. Front Plant Sci 7:371. https://doi.org/10.3389/fpls.2016.00371
Jaswanthi N, Krishna MSR, Lakshmi Sahitya U, Suneetha P (2019) Apoplast proteomic analysis reveals drought stress-responsive protein datasets in chilli (Capsicum annuum L.). Data Brief 25:104041. https://doi.org/10.1016/j.dib.2019.104041
Kaur G, Singh S, Singh H, Chawla M, Dutta T, Kaur H, Bender K, Snedden WA, Kapoor S, Pareek A, Singh P (2015) Characterization of peptidyl-prolyl cis-trans isomerase-and calmodulin-binding activity of a cytosolic Arabidopsis thaliana cyclophilin AtCyp19-3. PLoS One 10:e0136692. https://doi.org/10.1371/journal.pone.0136692
Kim SG, Lee JS, Kim JT, Kwon YS, Bae DW, Bae HH, Son BY, Baek SB, Kwon YU, Woo MO, Shin S (2015) Physiological and proteomic analysis of the response to drought stress in an inbred Korean Maize line. Plant Omics 8:159. https://www.pomics.com/shin_8_2_2015_159_168.pdf
Koiwa H, Bressan RA, Hasegawa PM (1997) Regulation of protease inhibitors and plant defense. Trends Plant Sci 2:379–84. https://doi.org/10.1016/S1360-1385(97)90052-2
Liu J, Blaylock LA, Harrison MJ (2004) cDNA arrays as a tool to identify mycorrhiza-regulated genes: identification of mycorrhiza-induced genes that encode or generate signaling molecules implicated in the control of root growth. Can J Bot 82:1177–1185. https://doi.org/10.1139/b04-048
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2 – ∆∆CT method. Methods 25:402–408. https://doi.org/10.1006/meth.2001.1262
Parrotta L, Guerriero G, Sergeant K, Cai G, Hausman JF (2015) Target or barrier? The cell wall of early-and later-diverging plants vs cadmium toxicity: differences in the response mechanisms. Front Plant Sci 6:133. https://doi.org/10.3389/fpls.2015.00133
Ramanjulu S, Kaiser W, Dietz KJ (1999) Salt and drought stress differentially affect the accumulation of extracellular proteins in barley. Z Naturforsch C 54:337–347. https://doi.org/10.1515/znc-1999-5-608
Ramegowda V, Senthil-Kumar M, Ishiga Y, Kaundal A, Udayakumar M, Mysore KS (2013) Drought stress acclimation imparts tolerance to Sclerotinia sclerotiorum and Pseudomonas syringae in Nicotiana benthamiana. Int J Mol Sci 14:9497–9513. https://doi.org/10.3390/ijms14059497
Romero FM, Rossi FR, Gárriz A, Carrasco P, Ruíz OA (2019) A bacterial endophyte from apoplast fluids protects canola plants from different phytopathogens via antibiosis and induction of host resistance. Phytopathology 109:375–383. https://doi.org/10.1094/PHYTO-07-18-0262-R
Sahitya UL, Krishna M, Deepthi R (2018) Biochemical and physiological changes induced by water stress in hot pepper (Capsicum annum L.) genotypes. Plant Cell Biotechnol. Mol Biol 19:179–195
Sahitya UL, Krishna MSR, Suneetha P (2019) Integrated approaches to study the drought tolerance mechanism in hot pepper (Capsicum annuum L.). Physiol. Mol Biol Pla 25:637–647. https://doi.org/10.1007/s12298-019-00655-7
Sang YL, Xu M, Ma FF, Chen H, Xu XH, Gao XQ, Zhang XS (2012) Comparative proteomic analysis reveals similar and distinct features of proteins in dry and wet stigmas. Proteomics 12:1983–1998. https://doi.org/10.1002/pmic.201100407
Sharma AD, Wajapeyee N, Yadav V, Singh P (2004) Stress-Induced Changes in Peptidyl-Prolyl cis-trans Isomerase Activity of Sorghum bicolor Seedlings. Biol Plantarum 47:367–371. https://doi.org/10.1023/B:BIOP.0000023879.74558.48
Simões I, Faro C (2004) Structure and function of plant Aspartic proteinases. Eur J Biochem 271:2067–2075. https://doi.org/10.1111/j.1432-1033.2004.04136.x
Taşgın E, Atıcı Ö, Nalbantoğlu B, Popova LP (2006) Effects of salicylic acid and cold treatments on protein levels and on the activities of antioxidant enzymes in the apoplast of winter wheat leaves. Phytochem 67:710–715. https://doi.org/10.1016/j.phytochem.2006.01.022
Zhang L, Tian LH, Zhao JF, Song Y, Zhang CJ, Guo Y (2009) Identification of an apoplastic protein involved in the initial phase of salt stress response in rice root by two-dimensional electrophoresis. Plant Physiol 149:916–928. https://doi.org/10.1104/pp.108.131144
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Nallamothu, J., Motukuri, S.R.K., Uppluri, L.S. et al. Comparative expression profiling reveals the role of the hot pepper apoplast under drought conditions. Biologia 76, 375–380 (2021). https://doi.org/10.2478/s11756-020-00579-8
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DOI: https://doi.org/10.2478/s11756-020-00579-8