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Determination of antibiotics and detergent residues in decellularized tissue-engineered heart valves using LC–MS/MS

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Abstract

Residual chemicals that are presented during tissue processing in human tissue banks can be a risk for the allograft recipient. Determine the residual concentrations of the antibiotics and detergent used in the process of human decellularized tissue-engineered heart valves stored in isotonic saline solution up to 18 months. A total of 24 human decellularized allografts were stored in sterile sodium chloride and analyzed immediately after the decellularization process (0 months) and after storage for 6, 12, and 18 months, which includes the use of sodium dodecyl sulfate (SDS) and antibiotics (cefoxitin, vancomycin hydrochloride, lincomycin hydrochloride, polymyxin B sulfate). These valves were used for suitability tests, the zone of inhibition evaluation, and direct contact cytotoxicity assay. The stock solution from 32 valves was used for LC–MS/MS analysis of antibiotics and SDS. Tissue samples from decellularized valves showed a zone of inhibition formation for S. aureus and B. subtilis, suggesting the presence of an inhibitory molecule in the tissue. Cytotoxicity tests were negative. Polymyxin B, vancomycin, and SDS were detected and quantified in human decellularized aortic and pulmonary allografts during all periods of the study. There were no traces of residual cefoxitin and lincomycin in the tissue stock solution. We found residual concentrations of the antibiotics and detergent used in the process of human decellularized tissue-engineered heart valves stored in isotonic saline solution up to 18 months.

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Funding

This work was supported by the Brazilian Ministry of Health (FNS 814611/2014).

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Authors and Affiliations

  1. Laboratory of Emerging Infectious Diseases, School of Medicine, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Curitiba, PR, 80215-901, Brazil

    Leticia Kraft, Victoria Stadler Tasca Ribeiro, Paula Hansen Suss & Felipe Francisco Tuon

  2. Human Tissue Bank, Pontifícia Universidade Católica do Paraná, Curitiba, PR, 80215-901, Brazil

    Luciana Cristina Ferretti de Nazareno Wollmann & Felipe Francisco Tuon

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  1. Leticia Kraft
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  2. Victoria Stadler Tasca Ribeiro
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  3. Luciana Cristina Ferretti de Nazareno Wollmann
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  4. Paula Hansen Suss
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  5. Felipe Francisco Tuon
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Correspondence to Felipe Francisco Tuon.

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Kraft, L., Ribeiro, V.S.T., de Nazareno Wollmann, L.C.F. et al. Determination of antibiotics and detergent residues in decellularized tissue-engineered heart valves using LC–MS/MS. Cell Tissue Bank 21, 573–584 (2020). https://doi.org/10.1007/s10561-020-09856-x

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  • DOI: https://doi.org/10.1007/s10561-020-09856-x

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