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Genome-wide identification and expression analysis of the Citrus malectin domain-containing receptor-like kinases in response to arbuscular mycorrhizal fungi colonization and drought

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Abstract

Malectin-like domain-containing receptor-like kinases (MRLKs) compose a big and diverse protein family in plants that plays significant roles in biotic and abiotic stress response. However, the MRLK family members in Citrus, as well as their roles during drought stress (abiotic stress) and arbuscular mycorrhizal fungi (AMF) colonization, remain relatively unknown. In the present study, we characterized the MRLK family genes in Citrus. By analyzing the presence of malectin-like domains and searching through the Citrus genome, we identified 47 CsMRLKs located within nine chromosomes, with most CsMRLKs mainly located on chromosome 1. These 47 CsMRLK family members were classified into 6 subfamilies with distinct malectin domains. Gene expression analysis showed that all 47 CsMRLKs were expressed in the roots and exhibited differential expression patterns. During the drought stress response, CsMRLK6, 7, 8, 9, 10, 11, 19, 25, 26, and 40 were downregulated. AMF colonization downregulated the expression of CsMRLK6, 10, 11, 12, 14, 17, 20, 30, 35, 43, and 47 and upregulated the expression of CsMRLK2, 5, 13, 21, 22, 28, 29, 32, 38, 41, 45, and 46. In addition, AMF colonization and drought stress showed a synergistic effect, in which the downregulated genes became even more weakened and the upregulated genes became even more enhanced. The present study offers useful perception into the Citrus MRLK gene family and its responses in AMF colonization and drought stress.

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Acknowledgements

This work was supported by the Scientific Research Foundation for doctor of Yangtze University (No. 802100270303).

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Authors and Affiliations

  1. College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, People’s Republic of China

    Chun Luo, Fei Zhang, Dejian Zhang, Chunyan Liu, Qiangsheng Wu & Bo Shu

  2. South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Science, Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture, Zhanjiang, 524091, People’s Republic of China

    Bo Shu

  3. Wuhan Forestry Station, Wuhan, 432200, People’s Republic of China

    Qiaofeng Sun

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  1. Chun Luo
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  4. Dejian Zhang
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  5. Chunyan Liu
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  6. Qiangsheng Wu
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Contributions

Chun Luo: conceived the experiments and wrote the manuscript. Chun Luo, Bo Shu, Fei Zhang: carried out the experiments. Chun Luo, Qiaofeng Sun, Dejian Zhang, Chunyan Liu: analyzed the data. Qiangsheng Wu, Bo Shu: critically reviewed the manuscript.

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Correspondence to Bo Shu.

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Supplementary Fig. S1

Phylogenetic tree of CsMRLK proteins and four other SYMRK proteins (TIFF 1106 kb)

Supplementary Fig. S2

Analysis and distribution of conserved motifs in CsMRLK proteins. (TIFF 2842 kb)

Supplementary Table S1

Primer sequences used for qRT-PCR analysis. (DOCX 14 kb)

Supplementary Table S2

Fragments per kilobase of exon model per million mapped reads (FPKM) values of each treatment. (XLSX 18 kb)

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Luo, C., Sun, Q., Zhang, F. et al. Genome-wide identification and expression analysis of the Citrus malectin domain-containing receptor-like kinases in response to arbuscular mycorrhizal fungi colonization and drought. Hortic. Environ. Biotechnol. 61, 891–901 (2020). https://doi.org/10.1007/s13580-020-00273-3

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