Skip to main content
SpringerLink
Log in

Cutaneous fibropapilloma in Egyptian-farmed gilthead seabream (Sparus aurata; Linnaeus, 1758)

  • Published:
Aquaculture International Aims and scope Submit manuscript

Abstract

In this study, three farmed Gilthead seabream exhibited tumor-like mass above the left pectoral fin. The mass measured 9 mm in length, 8 mm in width. Morphometrically, the mass was fleshy, raised, circumscribed, and rosy colored with grayish-white center. The affected fish showed poor growth with 280 g average body weight after 30 months rearing period. Internally, moribund fish exhibited pale kidney/liver, mottled spleen with remarkable visceral adhesions. The presumptive bacteriological and conventional biochemical assays revealed that fish were clinically infected with Photobacterium damselae subspecies piscicida. The histopathological picture of the tumor-like mass concurred with the standard cellular criteria of fibropapilloma. In brief, the epidermal papilloma was denoted by the remarkable hyperplasia of epidermis together with eosinophilic intracytoplasmic inclusion bodies and prominent mitosis in the hyperplastic cells. Ultimately, the proliferated dermal fibroblasts appeared whirling and elongated with spindle nuclei. Such pathological alterations have confirmed a typical form of dermal fibroma upon positively reacted with Masson’s trichrome stain. In the spleen, characteristic granulomatous reactions were observed especially in the area of melanomacrophage centers where the granuloma showed different developmental stages. The occurrence of skin tumors in farmed marine fish is an alarm for the critical impacts of the erratic usage of pesticides on marine aquaculture presenting a staggering prototype for the growing aquatic environmental pollution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Abdelsalam M, Elgendy MY, Shaalan M, Moustafa M, Fujino M (2017) Rapid identification of pathogenic streptococci isolated from moribund red tilapia (Oreochromis spp.). Acta Vet Hung 65(1):50–59

    Article  CAS  Google Scholar 

  • Anders K, Yoshimizu M (1994) Role of viruses in the induction of skin tumours and tumour-like proliferations of fish. Dis Aquat Org 19:215–232

    Article  Google Scholar 

  • Authman MMN, Zaki MS, Khallaf EA, Hossam H (2015) Use of fish as bio-indicator of the effects of heavy metals pollution. J Aquac Res Development 6:4. https://doi.org/10.4172/2155-9546.1000328

    Article  CAS  Google Scholar 

  • Campolo M, Postiglione G et al (2014) First electrosurgical excision of fibrosarcoma in goldfish (Carrassius auratus auratus). Bull. Eur. Ass. Fish. Pathol. 35(1):26–30

    Google Scholar 

  • Coffee LL, Casey JW, Bowser PR (2013) Pathology of tumours in fish associated with retroviruses: a review. Vet Pathol 50:390–403

    Article  CAS  Google Scholar 

  • EI-Jakee J, Elshamy S, Hassan A, Abdelsalam M et al (2019) Isolation and characterization of mycoplasmas from some moribund Egyptian fishes. Aquac Int 28:901–912. https://doi.org/10.1007/s10499-019-00502-2

    Article  CAS  Google Scholar 

  • Eisler R. (1988) Lead hazards to fish, wildlife, and invertebrates: a synoptic review; Contaminant hazard reviews, Biological Report 85 (1 .14)

  • Eissa AE, Abdelsalam M, Abumhara A et al (2015) First record of Vibrio vulnificus/Anisakis pegreffi concurrent infection in black scorpionfish (Scorpaena porcus) from the South Mediterranean basin. Res J Pharm Biol Chem Sci 6:1537–1548

    Google Scholar 

  • Eissa AE, Abolghait SK, Younis NA, Dessouki AA, el-Lamie MM, Abu Mhara AA, Abdelsalam M (2020) Myxobolus episquamalis infection in farmed flathead grey mullet Mugil cephalus L. and thin-lipped mullet Liza ramada. Aquacult Int 28:363–376. https://doi.org/10.1007/s10499-019-00467-2

    Article  CAS  Google Scholar 

  • El-mezayen MM, Rueda-roa DT, Essa MA, Muller-Karger FE, Elghobashy AE (2018) Water quality observations in the marine aquaculture complex of the Deeba Triangle, Lake Manzala, Egyptian Mediterranean coast. Environ Monit Assess 190:436

    Article  CAS  Google Scholar 

  • Franch R, Louro B, Tsalavouta M, Chatziplis D, Tsigenopoulos CS, Sarropoulou E, Antonello J, Magoulas A, Mylonas CC, Babbucci M, Patarnello T, Power DM, Kotoulas G, Bargelloni L (2006) A genetic linkage map of the hermaphrodite teleost fish Sparus aurata L. Genetics. 174(2):851–861. https://doi.org/10.1534/genetics.106.059014

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Freeman MA (2009) X-cell parasites in the European dab Limanda limanda are related to other X-cell organisms: a discussion on the potential identity of this new group of parasites. Parasitology 136:967–980

    Article  CAS  Google Scholar 

  • Garrity GM, Winters M, Searles DB (2001) Taxonomic outline of the prokaryotic genera. In Bergey’s Manual of Systematic Bacteriology ed. Garrity, G.M. Springer-Verlag, New York, NY: Bergey’s Manual Trust

  • Groff JM (2004) Neoplasia in fishes. Veterinary Clinics of North Amer- ica: Exotic Animal Practice 7(3):705–756

    Google Scholar 

  • Guyton KZ, Loomis D, Grosse Y, el Ghissassi F, Benbrahim-Tallaa L, Guha N, Scoccianti C, Mattock H, Straif K, International Agency for Research on Cancer Monograph Working Group, IARC, Lyon, France (2015) Carcinogenicity of tetrachlorvinphos , parathion , malathion , diazinon , and glyphosate. Lancet Oncol 16:490–491

    Article  Google Scholar 

  • Hall TA (1999) Bioedit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/ NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Harshbarger JC, Clark JB (1990) Epizootiology of neoplasms in bony fish of North America. Sci Total Environ 94(1–2):1–32

    Article  CAS  Google Scholar 

  • Jacobson ER, Buerge C, Williams B, Harris RK (1991) Herpesvirus in cutaneous fibropapillomas of the green turtle Chelonia mydas. Dis. Aquat. Org (12):1–6

  • Kandiel MMM, El-Asely AM et al (2013) Modulation of genotoxicity and endocrine disruptive effects of malathion by dietary honeybee pollen and propolis in Nile tilapia (Oreochromis niloticus). J Adv Res 5(6):671–684. https://doi.org/10.1016/j.jare.2013.10.004

    Article  PubMed  PubMed Central  Google Scholar 

  • Korkea-aho T (2007) Epidermal papillomatosis in roach ( Rutilus rutilus ) as an indicator of environmental stressors. Kuopio University Publications C. Natural and Environmental Sciences 223. 53

  • Korkea-aho TL, Vehniäinen ER, Kukkonen JVK, Taskinen J (2008) The effects of treated effluents on the intensity of papillomatosis and HSP70 expression in roach. Ecotoxicol Environ Saf 70:462–468

    Article  CAS  Google Scholar 

  • Lal B, Sarang MK, Kumar P (2013) Malathion exposure induces the endocrine disruption and growth retardation in the catfish, Clarias batrachus (Linn.). Gen Comp Endocrinol 181:139–145

    Article  CAS  Google Scholar 

  • Mahmoud AM, Aly SM, Diab AS, John G (2009) The role of ornamental goldfish Carassius auratus in transfer of some viruses and ectoparasites to cultured fish in Egypt: comparative ultrapathological studies. Afr J Aqua Sci 34(2):111–121

    Article  Google Scholar 

  • Mahmoud MA, Abdelsalam M et al (2016) Infectious bacterial pathogens, parasites and pathological correlations of sewage pollution as an important threat to farmed fishes in Egypt. Environ. Pollut 219:939–948

    Article  CAS  Google Scholar 

  • Mahmoud MA, Abd El-Rahim AH, Mahrous KF et al (2019) The impact of several hydraulic fracking chemicals on Nile tilapia and evaluation of the protective effects of Spirulina platensis. Environ Sci Pollut Res 26:19453–19467. https://doi.org/10.1007/s11356-019-05246-3

    Article  Google Scholar 

  • Manier JF, Raibaut A et al (1984) A calcified fibroma in the common carp, Cyprinus carpio. L J Fish Dis 7:283–292

  • Nermeen MA, Reham MA, Marzouk MS (2015) Molecular and immunohistochemical diagnosis of Photobacterium damselae subspecies piscicida during naturally occurring disease in Egypt. J World Aquacult Soc 46(6):583–595

    Article  CAS  Google Scholar 

  • Osorio CR, Collins MD et al (1999) 16S rRNA gene sequence analysis of Photobacterium damselae and nested PCR method for rapid detection of the causative agent of fish pasteurellosis. Appl. Environ. Microbiol 65:2942–2946

    Article  CAS  Google Scholar 

  • Osorio CR, Toranzo AE, Romalde JL, Barja JL (2000) Multiplex PCR assay for ureC and 16S rRNA genes clearly discriminates between both subspecies of Photobacterium damselae. Dis AquaOrganis 40:177–183

    CAS  Google Scholar 

  • Premdas PD, Metcalfe CD et al (2001) The effects of 17β-oestradiol, testosterone and tamoxifen on the development of papillomata in Catostomus commersoni. J Fish Biol 59:1056–1069

    CAS  Google Scholar 

  • Ramos P, Peleteiro M (2003) Three cases of spontaneous neoplasia in fish. Rev Port Cien Vet 98:77–80

    Google Scholar 

  • Roberts RJ (2012) Neoplasia of teleosts. In: Fish pathology, 4th edn. Baillere-Tindall, London, pp 177–185

    Chapter  Google Scholar 

  • Romalde JL (2002) Photobacterium damselae subsp. piscicida: an integrated view of a bacterial fish pathogen. Int Microbiol 5(1):3–9

  • Sahoo MK, Kumar PR et al (2017) Dermal fibroma in olive barb Systomus sarana ( Hamilton , 1822 ) : a case study. Indian J. Fish 64(1):95–97. https://doi.org/10.21077/ijf.2017.64.1.61340-17

    Article  Google Scholar 

  • Singaravel V, Gopalakrishnan A, Raja K, Rajkumar M, Ferguson HW (2018) Neoplasia in goldlined seabream, Rhabdosargus sarba (Forsskål, 1775). J Fish Dis 41:405–411

    Article  CAS  Google Scholar 

  • Sokkar SM, Mahmoud AM, Karima FM (2001) Histopathological and ultrastrucrural studies on melanomas in xiphophorus fishes during hybridization. Bull Eur Ass Fish Pathol 21(2):56–63

    Google Scholar 

  • Syasina IG, Sokolovsky AS, Phedorova M (1999) Skin tumours in Pleuronectes obscurus (Pleuronectidae) represent a complex combination of epidermal papilloma and rhabdomyosarcoma. Dis Aquat Org 39:49–57

    Article  Google Scholar 

  • Zabka TS, Haulena M, Puschner B, Gulland FMD, Conrad PA, Lowenstine LJ (2006) Acute Lead toxicosis in a harbor seal (Phoca vitulina richardsi ) consequent to ingestion of a Lead ishing sinker. J Wildl Dis 42(3):651–657

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt

    Alaa Eldin Eissa, Mohamed Abdelsalam & Nehal A. Younis

  2. Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt

    Mahmoud A. Mahmoud

  3. Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya

    Abdelsalam A. Abu Mhara

  4. Department of Preventive Medicine, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya

    Rabia A. El Zlitne

Authors
  1. Alaa Eldin Eissa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mohamed Abdelsalam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mahmoud A. Mahmoud
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nehal A. Younis
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Abdelsalam A. Abu Mhara
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rabia A. El Zlitne
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alaa Eldin Eissa.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical considerations

All standard national and international ethical guidelines dealing with fish handling, challenge and sampling were fully adopted by the research team (authors).

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Eissa, A.E., Abdelsalam, M., Mahmoud, M.A. et al. Cutaneous fibropapilloma in Egyptian-farmed gilthead seabream (Sparus aurata; Linnaeus, 1758). Aquacult Int 28, 2081–2091 (2020). https://doi.org/10.1007/s10499-020-00579-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10499-020-00579-0

Keywords

Search

Navigation