Abstract
Mangroves are critical marine habitats. High hydrogen sulphide (H2S) is a feature of these important ecosystems and its toxicity creates a challenge for mangrove inhabitants. The mangrove rivulus (Kryptolebias marmoratus) is a selfing, hermaphroditic, amphibious fish that can survive exposure to 1116 μM H2S in the wild. These fish rely on cutaneous respiration for gas and ion exchange when emerged. We hypothesized that the skin surface is fundamentally important in H2S tolerance in these mangrove fish by limiting H2S permeability. To test our hypothesis, we first disrupted the skin surface in one isogenic lineage and measured H2S tolerance and sensitivity. We increased water H2S concentration until emersion as a measure of the ability to sense and react to H2S, which we refer to as sensitivity. We then determined H2S tolerance by preventing emersion and increasing H2S until loss of equilibrium (LOE). The H2S concentration at emersion and LOE were significantly affected by disrupting the skin surface, providing support that the skin is involved in limiting H2S permeability. Capitalizing on their unique reproductive strategy, we used three distinct isogenic lineages to test the hypothesis that there would be genetic differences in H2S sensitivity and tolerance. We found significant differences in emersion concentration only among lineages, suggesting a genetic component to H2S sensitivity but not tolerance. Our study also demonstrated that external skin modifications and avoidance behaviours are two distinct strategies used to tolerate ecologically relevant H2S concentrations and likely facilitate survival in challenging mangrove habitats.
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The datasets generated and analyzed during the current study are available from Harvard dataverse (https://doi.org/10.7910/DVN/QRD1GH).
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Acknowledgements
This research was funded by a Natural Sciences and Engineering Research Council of Canada (NSERC) Graduate Scholarship, New Brunswick Innovation Foundation (NBIF) Science, Technology, Engineering and Mathematics scholarship (STEM) to K.E.M and NSERC Discovery and NBIF grants to S.C. The authors declare no conflict of interest.
Funding
This research was funded by a Natural Sciences and Engineering Research Council of Canada (NSERC) Graduate Scholarship, New Brunswick Innovation Foundation (NBIF) Science, Technology, Engineering and Mathematics scholarship (STEM) to K.E.M and NSERC Discovery and NBIF grants to S.C.
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Martin, K.E., Currie, S. Hydrogen sulphide sensitivity and tolerance in genetically distinct lineages of a selfing mangrove fish (Kryptolebias marmoratus). J Comp Physiol B 190, 761–770 (2020). https://doi.org/10.1007/s00360-020-01302-4
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DOI: https://doi.org/10.1007/s00360-020-01302-4