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Polyhydroxyalkanoate synthesis by bacteria isolated from landfill and ETP with pomegranate peels as carbon source

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Abstract

Many bacteria produce polyhydroxyalkanoates (PHAs) when exposed to stressful conditions. It is a known fact that PHAs have the potential to replace petrochemical-based plastics as they are biodegradable, biocompatible, and thermoprocessible materials. The study focusses on producing PHA from microbes isolated from polluted environments and pomegranate peels were utilized as a unique carbon source. This was done to ensure reduction in the cost of the substrate that has not yet been reported as a substrate for PHA production. A comparative study was also done with Cupriavidus necator, the reference strain. Out of many bacterial strains, isolated, eight of these were found to have ability to produce PHA. Pomegranate peel was substituted as carbon source in the medium and all bacterial isolates along with reference strain were used to test their ability to produce PHA from waste. Cupriavidus necator, the reference strain, yielded 71% PHA. Bacillus halotolerans DSM8802 yielded 83% at 1:1:: C:N ratio at 72 h.

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Acknowledgements

We are thankful to Dr. P. Shyama Raju, The Founder and Hon’ble Chancellor of Reva University; Dr. N. Ramesh, the Dean- Training, Placement and Planning and Dr. M. Dhanamjaya, the Registrar of Reva University, Bangalore for constant support and encouragement. We are grateful to Sateesh. G. Muttagi, CEO; Dr. Siddalingeshwar. K. G, Quality Manager and Mr. Babu. S, Business Development Head of Scientific and Industrial Research Center (NABL) Laboratories, Bangalore for providing the lab facilities and for their constant support and assistance. We are grateful to Prof. K.D. Vachhrajani, Head of the Department, Department of Environmental Studies and Prof. H.R. Kataria, Dean, Faculty of Science, The Maharaja Sayajirao University of Baroda for encouragement. We express our immense gratitude towards the GSBTM (Gujarat State Biotechnology Mission) for providing the funding through BIOTHON grant.

Funding

GSBTM, Government of Gujarat, Department of Science and Technology, India.

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Authors and Affiliations

  1. Department of Biotechnology, REVA University, Rukmini Knowledge Park, Kattigenahalli, Bangalore, 560064, India

    Vijay Rayasam

  2. Parul Institute of Applied Sciences (PIAS), Parul University, Post Limda, Waghodia, Vadodara, Gujarat, 391760, India

    Pooja Chavan

  3. Department of Environmental Studies, Faculty of Science, Sir CV Raman Building, The Maharaja Sayajirao University, Sayajigunj, Vadodara, Gujarat, 390002, India

    Tarika Kumar

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  1. Vijay Rayasam
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  2. Pooja Chavan
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  3. Tarika Kumar
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Correspondence to Tarika Kumar.

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Communicated by Erko Stackebrandt.

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Rayasam, V., Chavan, P. & Kumar, T. Polyhydroxyalkanoate synthesis by bacteria isolated from landfill and ETP with pomegranate peels as carbon source. Arch Microbiol 202, 2799–2808 (2020). https://doi.org/10.1007/s00203-020-01995-9

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  • DOI: https://doi.org/10.1007/s00203-020-01995-9

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