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LINC00324 affects non-small cell lung cancer cell proliferation and invasion through regulation of the miR-139-5p/IGF1R axis

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Abstract

Long non-coding RNAs (lncRNAs) are proved to perform critical function in regulating cancer cell behavior. It is reported that LINC00324 promotes lung adenocarcinoma development by regulating miR-615-5p/AKT1 axis. This study aimed to demonstrate whether LINC00324 participates in non-small cell lung cancer (NSCLC) pathogenesis through other molecular mechanism. Relative mRNA, lncRNA, and microRNA levels were analyzed using quantitative real-time–polymerase chain reaction (qRT-PCR). Western blot was used to detect protein level. MTT assay shown proliferation ability and transwell assay shown invasive ability. Luciferase reporter assay illustrated the interaction between RNA molecules. In NSCLC, the high expression of LINC00324 had correlation with the poor prognosis. LINC00324 promoted the proliferation and invasion of NSCLC cells while miR-139-5p inhibited these behaviors. LINC00324 overexpression promoted insulin-like growth factor 1 receptor (IGF1R) expression via absorbing miR-139-5p. The tumor-promoting effects of LINC00324 were attenuated through miR-139-5p overexpression. Highly expressed LINC00324 in NSCLC through sponged miR-139-5p to elevate IGF1R expression and promoted cell proliferation and invasion. This research demonstrated that LINC00324 is a potential NSCLC diagnosis and therapy target.

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Author notes

  1. Meiqing Zhang and Baoquan Lin have contributed equally to this work.

Authors and Affiliations

  1. Cardiothoracic Surgery, 900 Hospital of the Joint Logistics Team, No.156 West Second-beltway Road, Fuzhou, 350025, Fujian, China

    Meiqing Zhang, Baoquan Lin, Yaming Liu, Tengfei Huang, Mengmeng Chen, Duohuang Lian, Shilong Deng & Congwen Zhuang

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  1. Meiqing Zhang
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  2. Baoquan Lin
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  3. Yaming Liu
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Correspondence to Congwen Zhuang.

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This experiment was approved by Ethics Committee of the Fujian Cancer Hospital & Fujian Medical University Cancer Hospital.

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Zhang, M., Lin, B., Liu, Y. et al. LINC00324 affects non-small cell lung cancer cell proliferation and invasion through regulation of the miR-139-5p/IGF1R axis. Mol Cell Biochem 473, 193–202 (2020). https://doi.org/10.1007/s11010-020-03819-2

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  • DOI: https://doi.org/10.1007/s11010-020-03819-2

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