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Food-grade expression of nattokinase in Lactobacillus delbrueckii subsp. bulgaricus and its thrombolytic activity in vitro

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Abstract

Objectives

To produce nattokinase in a food-grade expression system and evaluate its thrombolytic activity in vitro.

Results

No nattokinase activity from reconstituted strains was observed in simulated gastric juice, but the enzyme was stable in intestinal fluid, the relative activity of which was found to be 60% after 4 h. Due to the nattokinase being produced intracellularly by recombinant bacterial strains, the persistence of the bacteria in gastric juice ensured transmission of the nattokinase into intestinal juice. Because of subsequent disintegration of the bacteria, the highest nattokinase activity was observed after 3 h at approximately 32%, following its carriage within the recombinant strains to the intestinal fluid.

Conclusions

This study demonstrated that nattokinase from recombinant strains exhibited good thrombolytic activity in vitro and may be used by the dairy fermentation industry for the development of novel thrombolytic functional foods.

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Acknowledgements

This work was supported by the National Major Project for Transgenic Organism Breeding of China (2019ZX08015-002), the National Natural Science Foundation of China (Grant No. 31301520), the Natural Science Foundation of Hebei Province (Grant Nos. C2016204129, C2017204094) and the Chinese High Technology Program (863) (2006AA10Z317).

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Author notes

  1. Chen Li, Zaihui Du, and Shaoqing Qi have contributed equally to this study.

Authors and Affiliations

  1. College of Food Science and Technology, Agricultural University of Hebei, Baoding, China

    Chen Li, Zaihui Du, Shaoqing Qi, Xin Zhang, Yangping Zhou, Haiqiang Lu, Xinxi Gu & Hongtao Tian

  2. New Hope Tensun (Hebei) Dairy Co., Ltd, Baoding, China

    Miaoshu Wang

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  1. Chen Li
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  2. Zaihui Du
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  3. Shaoqing Qi
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  4. Xin Zhang
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  5. Miaoshu Wang
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  9. Hongtao Tian
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Correspondence to Hongtao Tian.

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Li, C., Du, Z., Qi, S. et al. Food-grade expression of nattokinase in Lactobacillus delbrueckii subsp. bulgaricus and its thrombolytic activity in vitro. Biotechnol Lett 42, 2179–2187 (2020). https://doi.org/10.1007/s10529-020-02974-2

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  • DOI: https://doi.org/10.1007/s10529-020-02974-2

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