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Genome characterization of brugmansia latent virus, a novel tobamovirus

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Abstract

A novel tobamovirus, brugmansia latent virus (BrLV), was discovered during a study of brugmansia (Brugmansia spp.) in the living collections held at the Royal Botanic Gardens, Kew. Here, we report the complete genome sequence of BrLV, which is 6,397 nucleotides long and contains the four open reading frames (RNA-dependent RNA polymerase, methyltransferase/helicase, movement, and coat proteins) typical of tobamoviruses. The complete genome sequence of BrLV shares 69.7% nucleotide sequence identity with brugmansia mild mottle virus (BrMMV) and 66.7 to 68.7% identity with other tobamoviruses naturally infecting members of the Solanaceae plant family. Phylogenetic analysis of the complete genome nucleotide sequence and the deduced amino acid sequences of the four tobamovirus proteins place BrLV in a subcluster with BrMMV within the Solanaceae-infecting tobamovirus subgroup as a new species.

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Acknowledgements

We thank Sara Redstone (Quarantine Officer) and Tom Pickering (Nursery Supervisor), Royal Botanic Gardens, Kew, for horticultural expertise and access to specimens, and Carrie Vanderspool and Shelby Hanson, USDA-ARS, for excellent technical assistance.

Funding

Financial support was provided in part by a Kew Pilot Award to A.S-B. and The Banack Family Partnership Endowed Teaching Chair in Agriculture to T.D.

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Authors and Affiliations

  1. Royal Botanic Gardens Kew, Richmond, Surrey, TW9 3AB, UK

    Alison S. Scott-Brown & Dion S. Devey

  2. Biology Department, Indian River State College, 3209 Virginia Avenue, Fort Pierce, FL, 39481, USA

    Tom D’Elia

  3. University of Florida, North Florida Research and Education Center, 155 Research Road, Quincy, FL, 32351, USA

    Joseph E. Funderburk

  4. USDA, ARS, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL, 34945, USA

    Scott Adkins

Authors
  1. Alison S. Scott-Brown
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  2. Tom D’Elia
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  3. Dion S. Devey
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  4. Joseph E. Funderburk
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  5. Scott Adkins
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Corresponding authors

Correspondence to Alison S. Scott-Brown or Scott Adkins.

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Handling Editor: Stephen John Wylie.

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Scott-Brown, A.S., D’Elia, T., Devey, D.S. et al. Genome characterization of brugmansia latent virus, a novel tobamovirus. Arch Virol 165, 2389–2392 (2020). https://doi.org/10.1007/s00705-020-04718-z

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  • DOI: https://doi.org/10.1007/s00705-020-04718-z

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