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Sperm antioxidant system in ocellate river stingray Potamotrygon motoro at transition from seminal vesicle to cloaca

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Abstract

The importance of reactive oxygen species and the antioxidant system in sperm biology has been recognized for different bony fishes but nothing is known in this regard for chondrichthyans. For the first time for cartilaginous fishes, the enzymatic antioxidant system was shown herein to be present in both fractions of sperm (spermatozoa and seminal fluid) collected from two different places (seminal vesicle and cloaca). In internally fertilizing freshwater ocellate river stingray, Potamotrygon motoro, the activity of superoxide dismutase and glutathione peroxidase was not changed upon sperm transition from the seminal vesicle to the cloaca. The activity of catalase was significantly increased for both sperm fractions at transition from the seminal vesicle to the cloaca (1.6 times for spermatozoa and 1.9 times for seminal fluid). The role of the sperm antioxidant system for different aspects of internal fertilization is discussed. The presented results are the initiatory step in uncovering the biochemical events of internal reproduction in Chondrichthyes.

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The original data of the study are available on request.

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Acknowledgments

The authors are grateful to the staff of LINEO (Laboratory of Neotropical Ichthyology), Department of Biology and Zootechny, São Paulo State University, Ilha Solteira, SP, Brazil, for their technical support and provision of facilities.

Funding

Funding was provided by the Czech Science Foundation (No. 16-03754S), by the Ministry of Education, Youth and Sports of the Czech Republic—project “CENAKVA” (LM2018099), by project Biodiversity (CZ.02.1.01./0.0/0.0/16_025/0007370 Reproductive and genetic procedures for preserving fish biodiversity and aquaculture), and by the Grant Agency of the University of South Bohemia in Ceske Budejovice (125/2016/Z).

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Authors and Affiliations

  1. Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zátiší 728/II, 389 25, Vodňany, Czech Republic

    Viktoriya Dzyuba, Marek Rodina & Borys Dzyuba

  2. Neotropical Ichthyology Laboratory (LINEO), Department of Biology and Zootechny, Ilha Solteira School of Engineering, São Paulo State University, Monção Street, 226, Ilha Solteira, SP, 15385-000, Brazil

    Alexandre Ninhaus-Silveira & Rosicleire Veríssimo-Silveira

Authors
  1. Viktoriya Dzyuba
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  2. Alexandre Ninhaus-Silveira
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  3. Rosicleire Veríssimo-Silveira
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  4. Marek Rodina
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  5. Borys Dzyuba
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Contributions

Viktoriya Dzyuba, Borys Dzyuba, and Marek Rodina designed the experiments; Alexandre Ninhaus-Silveira and Rosicleire Veríssimo-Silveira provided the facilities for fish maintenance and participated in sample collection; Viktoriya Dzyuba and Borys Dzyuba performed the experiments and analyzed the experimental data; Viktoriya Dzyuba wrote the manuscript with input from all the authors; Borys Dzyuba, Marek Rodina, Alexandre Ninhaus-Silveira, and Rosicleire Veríssimo-Silveira revised the manuscript. All the authors read and approved the final version of the manuscript.

Corresponding author

Correspondence to Viktoriya Dzyuba.

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The authors declare that they have no conflict of interest.

Ethics approval

All manipulations with animals were performed in accordance with the authorization for the use of experimental animals (Reference number: 2293/2015-MZE-17214 (16OZ22302/2014-17214), Czech Republic) and approved by the local Ethics Commission on Animal Use (CEUA-FEIS/UNESP 14/2018).

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Dzyuba, V., Ninhaus-Silveira, A., Veríssimo-Silveira, R. et al. Sperm antioxidant system in ocellate river stingray Potamotrygon motoro at transition from seminal vesicle to cloaca. Fish Physiol Biochem 46, 1975–1980 (2020). https://doi.org/10.1007/s10695-020-00848-y

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