Abstract
Objective
To evaluate the catalytic esterification performance of proteases in micro-aqueous systems and to study the suitable conditions for maintaining protease activity.
Results
It was found that the protease showed better enzyme catalytic activity in the micro-aqueous phase containing 4% boric acid-borax buffer than that of the pure organic phase. The protease activity was easily activated by 0.20 M boric acid-borax buffer, and the enzyme activity was still high for a long time in alkaline environment (pH 8.40–9.60) and under the temperature of 40–55 °C. Experiments using protease and Candida lipase to synthesize sucrose-6-ethyl ester showed that protease had better esterification activity than Candida lipase in the micro-aqueous phase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- Ms :
-
Sucrose mass
- Mp :
-
Products mass
- Sp :
-
Products peak area
- Ss :
-
Sucrose peak area
References
Cao X, Ni Y, Zhang A, Xu S, Chen KQ, Ouyang PK (2017) Encapsulation of enzymes in metal ion-surfactant nanocomposites for catalysis in highly polar solvents. Chem Commun 53:31–34. https://doi.org/10.1039/c7cc00607a
Han YW, Liu GM, Huang DY, Qiao BJ, Chen LP, Guan LH, Mao DB (2011) Study on the synthesis of sucrose-6-acetate catalyzed by fructosyltransferase from Aspergillus oryzae. New Biotechnol 28:14–18. https://doi.org/10.1016/j.nbt.2010.07.007
Halling PJ (1989) Organic liquids and biocatalysts: theory and practice. Trendsin Biotechnol 7:50–52
Karboune S, Appanah N, Khodaei N, Tian F (2018) Enzymatic synthesis of fructooligosaccharides from sucrose by endo-inulinase-catalyzed transfructosylation reaction in biphasic systems. Process Biochem 69:82–91. https://doi.org/10.1016/j.procbio.2018.03.010
Liu Y, Chen DW, Yan YJ, Peng C, Xu L (2011) Biodiesel synthesis and conformation of lipase from Burkholderia cepacia in room temperature ionic liquids and organic solvents. Bioresource Technol 102(22):10414–10418. https://doi.org/10.1016/j.biortech.2011.08.056
Li LL, Feng W, Pan KH (2013) Immobilization of lipase on amino-cyclodextrin functionalized carbon nanotubes for enzymatic catalysis at the ionic liquidorganic solvent interface. Colloid Surface B 102:124–129. https://doi.org/10.1016/j.colsurfb.2012.07.050
Li X, Li D, Wang W, Durrani R, Yang B, Wang YH (2016) Imbobilization of SMG1-F278N lipase onto a novel epoxy resin: characterization and its application in synthesis of partial glycerides. J Mol Catal B Enzym 133:154–160. https://doi.org/10.1016/j.molcatb.2016.08.011
Qian JQ, Qin DH, Xie XM, Zhou WW (2010) Study on enzyme-assisted aqueous extraction of oil from soybean. J Sci Ind Res India 69:860–865
Ritthitham S, Wimmer R, Pedersen LH (2011) Polar co-solvents in tertiary alcohols effect initial reaction rates and regio-isomeric ratio ranging from 1.2 to 2.2 in a lipase catalysed synthesis of 6-O- and 6'-O-stearoyl sucrose. Process Biochem 46:931–935. https://doi.org/10.1016/j.procbio.2011.01.004
Sen S, Venkata Dasu V, Dutta K, Mandal B (2011) Characterization of a novel surfactant and organic solvent stable high-alkaline protease from new Bacillus pseudofirmus SVB1. J Microbiol 6:769–783. https://doi.org/10.3923/jm.2011.769.783
Tran DT, Chen CL, Chang JS (2013) Effect of solvents and oil content on direct transesterification of wet oil-bearing microalgal biomass of Chlorella vulgaris ESP-31 for biodiesel synthesis using immobilized lipase as the biocatalyst. Bioresour Technol 135:213–221. https://doi.org/10.1016/j.biortech.2012.09.101
Wei T, Jia WW, Yu XA, Mao DB (2016) Enhancement of enzymatic synthesisof sucrose 6-acetate with Aspergillus oryzae fructosyltransferase using ionic liquid as a cosolvent. J Mol Catal B Enzym 123:100–106. https://doi.org/10.1016/j.molcatb.2015.11.016
Yan WW, Wang NN, Zhang PM, Zhang JJ, Wu SC, Zhu Y (2016) Analysis ofsucrose acetates in a crude 6-O-acetyl sucrose product by on-line hydrolysis-high-performanc-e liquid chromatography with pulsed amperometric detection. J Chromatogr A 1449:71–77. https://doi.org/10.1016/j.chroma.2016.04.070
Yang XE, Zheng P, Ni Y, Sun ZH (2012) Highly efficient biosynthesis of sucrose-6-acetate with cross-linked aggregates of Lipozyme TL 100 L. J Biotechnol 161:27–33. https://doi.org/10.1016/j.jbiotec.2012.05.014
Zhong X, Qian JQ, Liu M, Ma LZ (2013) Candida rugosa lipase-catalyzed synthesis of sucrose-6-acetate in a 2-butanol/buffer two-phase system. Eng Life Sci 13:563–571. https://doi.org/10.1002/elsc.201200170
Zhong X, Qian JQ, Guo H, Hu YY, Liu M (2014) Biosynthesis of sucrose-6-acetate catalyzed by surfactant-coated Candida rugosa lipase immobilized on sol-gel supports. Bioproc Biosyst Eng 37:813–818. https://doi.org/10.1007/s00449-013-1053-9
Acknowledgements
The authors would like to thank professor Junqing Qian for all the feedstock.
Funding
This research project was financially supported by China's National Key Research and Development Program (Grant Number 2016YFD0400803).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest regarding the publication of this paper.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Qian, J., Gou, L., Zhao, X. et al. Catalytic esterification performance of protease in micro-aqueous system. Biotechnol Lett 42, 2157–2167 (2020). https://doi.org/10.1007/s10529-020-02965-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-020-02965-3