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Dynamic modelling of growth and flavonoid production from Ocimum tenuiflorum suspension culture

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Abstract

A structured-segregated dynamic model for biomass growth, sucrose utilization and flavonoid production in Ocimum tenuiflorum suspension culture is proposed, considering a dynamic heterogeneous population of viable active, viable nonactive and dead cell. The sucrose hydrolysis (into glucose and fructose), substrate uptake by biomass and intracellular flavonoid production are modelled using Contois kinetics, a competitive double-substrate Monod, and Luedeking–Piret model, respectively. The conversion of active to viable-nonactive biomass has been formulated as a function of the total substrate and biomass concentrations. Parameters for the dynamic model are evaluated while minimizing the sum of square errors between modelled and measured biomass, cell viability, glucose, fructose and intracellular flavonoid contents. Bootstrap confidence intervals and dynamic relative sensitivity analysis of these model parameters are presented. The knowledge gained from the population-based model in plant suspension culture can provide the basic framework for prediction and optimization of the bioprocess system for phytochemical production.

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Abbreviations

X:

Biomass concentration (g.l−1)

S:

Substrate concentration (g.l−1)

K:

Half saturation constant or Contois parameter (g.l−1)

P:

Product concentration (g.l−1)

m:

Mass conversion factor from sucrose to glucose or fructose (g.g−1)

Y:

Yield coefficient (g.g−1)

v:

Cell viability (g.g−1)

τ:

Function for activity loss

ϕ:

Biomass equivalent of substrate in activity loss

µ:

Specific growth rate (d−1)

k:

Rate constant for activity loss or death (d−1)

α:

Growth associated product constant (g.g−1)

β:

Growth nonassociated product constant (g.g−1.d−1)

γ:

Product release coefficient in cell lysis (g.g−1)

a:

Active-viable cell

n:

Nonactive-viable cell

d:

Dead cell

t:

Total cell

ac:

Activity loss

G:

Glucose

F:

Fructose

L:

Cell lysis

h:

Substrate hydrolysis

c:

Contois function

gs:

Conversion of sucrose to glucose

fs:

Conversion of sucrose to fructose

X/SG :

Biomass from glucose

X/SF :

Biomass from fructose

P/SG :

Product from glucose

P/SF :

Product from fructose

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Acknowledgements

This work is financially supported by Karunya Institute of Technology and Sciences. Standard ethical and professional conduct have been followed.

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  1. Department of Biotechnology, Karunya Institute of Technology and Sciences, Coimbatore, 641114, India

    Anu Jacob, Biswanath Mahanty & Jibu Thomas

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Jacob, A., Mahanty, B. & Thomas, J. Dynamic modelling of growth and flavonoid production from Ocimum tenuiflorum suspension culture. Bioprocess Biosyst Eng 43, 2053–2064 (2020). https://doi.org/10.1007/s00449-020-02394-6

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