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Sphingomonas hominis sp. nov., isolated from hair of a 21-year-old girl

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Abstract

A Gram-stain-negative, aerobic, motile strain, HHU CXWT, was isolated from hair of a healthy 21-year-old female student of Hohai University, Nanjing, China. The 16S rRNA gene sequence analysis indicated that HHU CXWT represents a member of the genus Sphingomonas with the highest sequence similarity (97.6%) to the type strain S. aquatilis JSS7T. HHU CXWT grew at 4–35 °C and pH 6–8, with optimum growth at 28 °C and pH 7. Tolerance to NaCl was up to 2% (w/v) with optimum growth in 0.5–1.0% NaCl. The major fatty acids were C16:0, C17:1ω6c, C18:1ω7c11-methyl, summed feature 3 (C16:1ω7c and/or C16:1ω6c), and summed feature 8 (C18:1ω7c and/or C18:1ω6c). The predominant isoprenoid quinone was ubiquinone-10. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), sphingoglycolipid (SGL), phosphatidylinositol mannosides (PIM), and an unidentified glycolipid (GL). The DNA G + C content was 67.1%. The average nucleotide identity (ANI) values and digital DNA–DNA hybridization (dDDH) between HHU CXWT and closely related members of the genus Sphingomonas were all below the cut-off level (95–96% and 70%, respectively) for species delineation. On the basis of the phenotypic, phylogenetic and chemotaxonomic characterizations, HHU CXWT represents a novel species of the genus Sphingomonas, for which the name Sphingomonas hominis sp. nov. is proposed. The type strain is HHU CXWT (= KCTC 72946T = CGMCC 1.17504T = MCCC 1K04223T).

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (Nos. 31900001 and 31972856). D-F Zhang was also supported by the Fundamental Research Funds for the Central Universities (2019B02014)

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Author notes

  1. Dao-Feng Zhang and Xi-Wen Cui contributed equally to this work.

Authors and Affiliations

  1. Institute of Marine Biology, College of Oceanography, Hohai University, Nanjing, People’s Republic of China

    Dao-Feng Zhang, Xi-Wen Cui, Zhe Zhao, Ai-Hua Zhang & Jian-Ke Huang

  2. State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Wen-Jun Li

  3. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People’s Republic of China

    Wen-Jun Li

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  1. Dao-Feng Zhang
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  4. Ai-Hua Zhang
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Contributions

DFZ and ZZ designed research and project outline. XWC, DFZ, and AHZ performed isolation, deposition and polyphasic taxonomy. DFZ, and JH performed genome analysis. DFZ and AHZ drafted the manuscript. WJL revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Dao-Feng Zhang or Wen-Jun Li.

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Zhang, DF., Cui, XW., Zhao, Z. et al. Sphingomonas hominis sp. nov., isolated from hair of a 21-year-old girl. Antonie van Leeuwenhoek 113, 1523–1530 (2020). https://doi.org/10.1007/s10482-020-01460-z

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