Structure
Volume 28, Issue 12, 1 December 2020, Pages 1358-1360.e2
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DStabilize: A Web Resource to Generate Mirror Images of Biomolecules

https://doi.org/10.1016/j.str.2020.07.014Get rights and content
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Highlights

  • Convert between L- and D-forms of peptides/protein structures

  • Apply custom geometrical transforms to structures of peptides/proteins

  • Generate retro-inverso forms of peptides

  • Webserver is available at http://dstabilize.bii.a-star.edu.sg/

Summary

Peptides comprising D-amino acids have been shown to be resistant to proteolysis. This makes them potential candidates as probes of cellular interactions, notably protein-biomolecule interactions. However, the empirical conversion of the amino acids that constitute a peptide from L-forms to D-forms will result in abrogation of the normal interactions made by the L-amino acids due to side-chain orientation changes that are associated with the changes in chirality. These interactions can be preserved by reversing the sequence of the D-peptide. We present a web server (http://dstabilize.bii.a-star.edu.sg/) that allows users to convert between L-proteins and D-proteins and for sequence reversal of D-peptides, along with the capability of performing other empirical geometric transforms. This resource allows the user to generate structures of interest easily for subsequent in silico processing.

Keywords

D-proteins
enantiomers
retero-inverso
protein structure transformation
peptide design

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These authors contributed equally

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