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Berberine attenuates Aβ-induced neuronal damage through regulating miR-188/NOS1 in Alzheimer’s disease

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Abstract

Alzheimer’s disease (AD) is a public health issue worldwide. Berberine (Ber) acts as the neuroprotective role in an animal experiment of AD. MicroRNA-188 (miRNA-188) was reported to be decreased in primary hippocampal neurons of mice. However, the roles and molecular basis of Ber and miRNA-188 in the treatment of AD need to be further explored. In this study, 5 μM Ber treatment has little effect on cell viability. Ber treatment or miR-188 overexpression expedited proliferation and inhibited caspase-3 activity and apoptotic rate in amyloid-beta (Aβ)-treated BV2 and N2a cells. MiR-188 was downregulated, and nitric oxide synthase 1 (NOS1) was upregulated in Aβ-induced BV2 and N2a cells. NOS1 worked as the target of miR-188. NOS1 overturned miR-188-induced effects on cell viability, caspase-3 activity, and apoptotic rate in Aβ-induced BV2 and N2a cells. Ber mitigated neuronal damage in Aβ-induced BV2 and N2a cells by miR-188/NOS1 axis. These results suggested that Ber accelerated cell viability and suppressed caspase-3 activity and apoptotic rate possible by miR-188/NOS1 pathway, implying the treatment of Ber as an underlying effective drug for AD patients.

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Authors and Affiliations

  1. Department of Psychiatry, Mental Health Center of Qingdao, Qingdao University, No. 299 Nanjing Road, Qingdao, 266033, Shandong, China

    Manman Chen, Lu Li, Chuanqin Liu & Lina Song

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  1. Manman Chen
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  2. Lu Li
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  3. Chuanqin Liu
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  4. Lina Song
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Correspondence to Lina Song.

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Chen, M., Li, L., Liu, C. et al. Berberine attenuates Aβ-induced neuronal damage through regulating miR-188/NOS1 in Alzheimer’s disease. Mol Cell Biochem 474, 285–294 (2020). https://doi.org/10.1007/s11010-020-03852-1

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  • DOI: https://doi.org/10.1007/s11010-020-03852-1

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